基于高密度SNP标记重构猪多品种群体系谱

系谱是动物育种的重要信息来源,本研究旨在探究高密度SNP标记重构系谱在生产群体中的效果,填补使用高密度SNP信息重构多品种、大规模真实生产猪群的空白。本研究利用Illumina GeneSeek GGP Porcine 50K芯片对四川某猪场2017—2021年出生的1 471头曾祖代纯种杜洛克猪(n=986)和长白猪(n=485)进行分型,通过共祖片段法(common ancestor fragment method)分析上述两个品种群体内基因组亲缘关系,由此分别重构两品种群体系谱。同时选取有个体芯片分型信息及系谱记录的115头种猪,通过严格控制生产操作流程保证其系谱记录准确无误,用以评价准确性。结果表明,基于共祖片段法利用基因组信息可以同时推断多代次、品种混合的真实生产群体内个体对间的共祖片段分布情况及比例,且较状态相同片段(identical by state, IBS)能更准确的区分个体间亲缘关系,借此判断个体间亲缘关系并进一步推断家系结构。同时该方法在115头种猪的验证群体中共推断出702对亲缘关系,包括系谱记录的全部亲子关系对(n=184)、全同胞对(n=175)、半同胞关...     

身份的焦虑--读《汤姆·琼斯》

身世之谜在<汤姆@琼斯>中起着表现主题、结构全书的重要作用.对身份问题的探讨体现了作者对虚伪社会与道德的批判及对真善美的憧憬,对后代作家产生了深远影响.     

甘蔗花叶病毒田间杂草寄主鉴定

Sugarcane mosaic virus (SCMV) is the prevalent virus causing maize dwarf mosaic in China. Here we collected 90 weed samples belonging to 15 species, among which Setaria viridis, Echinochloa crusgalli and Digitaria sanguinalis showed distinct mosaic symptom. The maize in-bred line B73 and cultivar Denghai 605 plants inoculated with extracts of S. viridis, E. crusgalli and D. sanguinalis showed distinct mosaic symptom in the systemically infected leaves at 10 days post-inoculation. ELISA results showed that samples of S. viridis, E. crusgalli and D. sanguinalis were positive to antibody against SCMV CP. The duplex-PCR showed that samples of S. viridis, E. crusgalli and D. sanguinalis were infected by SCMV which was not belonging to group IV. The SCMV ORF of 3 weed and one maize samples shared nucleotide and amino acid sequences identities of 93.1%-99.7%. Gene-flow analysis showed that the SCMV isolates from three weed samples had frequent gene flow with the SCMV isolates from maize in Shandong Province. Phylogenetic analysis showed that all the four SCMV isolates were clustered into group I. These results indicate that S. viridis, E. crusgalli and D. sanguinalis are weed reservoir of SCMV. These results provide theoretical guidance for the effective control of SCMV.     

高科技园区景观设计的企业文化表现研究

从展现企业文化的角度分析了景观设计,指出了景观设计应有其生命力、尊重地域性与历史性、重视具有地域特色的企业文化的塑造。探讨了高科技园区景观设计的企业文化表现。     

Looks can deceive: Molecular identity of an intraerythrocytic apicomplexan parasite in Australian gliders

Two yellow-bellied gliders (Petaurus australis) had an intraerythrocytic parasite closely related to the cyst-forming coccidia (Apicomplexa: Sarcocystidae). The parasitaemia persisted for 3 months or more but was observed to clear within 3 years in captivity. The parasite appears not to significantly debilitate its infected host. Traditionally, using morphological identification, the intraerythrocytic parasite would have been classified within the Hepatozoon species typically found in red blood cells. However, molecular diagnostic techniques targeting the parasite's SSU rDNA and LSU rDNA demonstrated the unusual identity of this blood parasite and disputed its identity as a haemogregarine parasite of the genus Hepatozoon. The sequence was compared with available sequences from diverse mammalian and non-mammalian blood parasites (malaria, piroplasms, hemosporidia and sarcosporidia). The intraerythrocytic blood parasite was found to be most closely related to the cyst-forming coccidia including Besnoitia spp., Cystoisospora spp., Hammondia spp., Hyaloklossia lieberkuehni, Neospora caninum, Sarcocystis spp. and Toxoplasma gondii. The life cycle of this intraerythrocytic parasite remains unknown. The presented DNA identification demonstrates its suitability for an improved identification of blood parasites.     

差异与同一：生态世界观的两个基点

人与自然既有同一性又有差异性,因而人与自然的同一性与差异性构成了生态世界观的两个基点。人与自然同一性是二者和谐共处的基础,人与自然差异性是确立人之主体地位的基础。人与自然的相互规定性是生态世界观的前提条件,自然选择与社会选择的相互规定性是生态世界观的现实基础。     

城管的自我认知现状及对策研究——以武汉市为例

指出了城管的自我认知对于提高城管的职业价值感、提高工作的热情和主动性有重要的影响作用,通过对自身职业认同感的认知、对执法方式的认知,及对目前社会处境的认知分析来了解城管自我认知的现状,并为如何改善提高城管自我认知能力提供了建议。     

两个马铃薯Y病毒黑龙江马铃薯分离物株系鉴定

为明确分离自黑龙江省克山县马铃薯上的2个病毒分离物KS4和KS7的分类地位,通过RTPCR扩增、克隆获得其基因组序列,利用重组分析程序包和最大似然法分别进行重组分析和系统发育分析。结果显示,分离物KS4和KS7的开放阅读框均有9 186个核苷酸,编码3 061个氨基酸,分离物KS4的核苷酸和氨基酸序列均与马铃薯Y病毒(potato virus Y,PVY)分离物Mb112一致率最高,分别为96.9%和98.4%;分离物KS7的核苷酸序列与PVY分离物12-94一致率最高,为97.4%,其氨基酸序列与PVY分离物SYR-Ⅱ-Be1一致率最高,为97.8%。重组分析表明,分离物KS4和KS7均为分离物N-605和Oz的重组体,其中KS4基因组5′-端的2 392个核苷酸来自分离物N-605,其余核苷酸来自分离物Oz;KS7基因组的第800～2 227个核苷酸和第5 637～8 950个核苷酸来自分离物N-605,其余核苷酸来自分离物Oz。系统发育分析发现,分离物KS4被聚类到N:O株系(PVY~(N:O)),分离物KS7被聚类到NTN株系(PVY~(NTN))b型。     

侵染红肉火龙果的蟹爪兰X病毒贵州分离物基因组分析

 通过高通量测序和RT-PCR扩增,克隆并分析蟹爪兰X病毒(Zygocactus virus X,ZyVX)贵州分离物ZyVX-GZ基因组序列。对表现病毒病症状的红肉火龙果植株进行高通量测序,获得4条ZyVX相关contig。RT-PCR扩增并拼接获得ZyVX-GZ基因组,全长为 6 567 nt,5′-UTR和 3′-UTR分别为60 nt和70 nt。含有5个ORF,分别编码复制酶蛋白、TGB1、TGB2、TGB3蛋白和外壳蛋白。ZyVX-GZ与P39和B1分离物基因组序列一致性均为91%。TGB1-3、外壳蛋白基因与大多数分离物核苷酸和推导的氨基酸序列一致性分别为95%～99%和96%～100%;复制酶基因的核苷酸和推导的氨基酸序列一致性分别为80%～89%和92%～97%。系统进化分析表明,ZyVX群体的遗传分化与寄主种类、地理分布不存在相关性。本研究结果丰富了ZyVX群体的基因组序列信息,为ZyVX的监测和防控提供了基础。     

Heisenberg型群上的广义Picone恒等式及其应用

利用Heisenberg型群上p-退化椭圆算子的广义Picone恒等式给出了Hardy不等式、 Sturmiam比较原理、 Liouville型定理和主特征值的单调性结论.讨论了具有奇异项的拟线性方程的弱解问题.