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芽孢杆菌BC98-Ⅰ抗真菌物质的初步分离及特性 总被引:1,自引:0,他引:1
BC98-Ⅰ是一株分离自沤肥浸渍液的蜡状芽孢杆菌,对蔬菜、瓜类、花卉枯萎病具良好的防治效果。它分泌产生的抗真菌物质的粗提物对黄瓜、西瓜、青椒枯萎菌有很强的拮抗作用,当浓度为10mg/mL时,抑菌圈直径分别达到20.33、20.22和22.77mm。该粗提物对高温高压、紫外光和蛋白酶均不敏感。同时,它在中性偏碱(pH8~9)的条件下活性最高;在极性溶剂如水、甲醇、乙醇中溶解度大,抑菌活性高。粗提液经Sephadex G100层析后,得到了两个洗脱峰,活性物质存在于第一峰中。抑菌机制试验表明:它可造成孢子萌发率降低、菌丝生长形态异常、分支加剧并产生大量泡囊。 相似文献
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Kyutaro Kishimoto Yoko Nishizawa Yutaka Tabei Masami Nakajima Tadaaki Hibi Katsumi Akutsu 《Journal of General Plant Pathology》2004,70(6):314-320
A class III chitinase gene (CHI2) is induced in cucumber plants (Cucumis sativa L.) in response to infection by pathogenic microorganisms. Infection of Botrytis cinerea, causal agent of gray mold disease on cucumber, also induces CHI2 expression. To investigate whether CHI2 is involved in resistance to gray mold disease, transgenic cucumber plants were produced to overexpress the CHI2 gene. One line was analyzed in detail in terms of disease resistance. The transgenic cucumber plant (CC2) constitutively expressed CHI2 and reduced the symptoms of B. cinerea for 4 days after inoculation compared with nontransgenic plants. However, this inhibitory effect was not absolute, and CC2 eventually developed serious disease symptoms. Chitinase activity of the crude extract from CC2 leaves was higher than that from nontransgenic plants. A high-molecular-weight fraction containing CHI2 from CC2 leaves had fungistatic activity against B. cinerea. Interestingly, the low-molecular-weight fraction from CC2 leaves with CHI2 removed also had fungistatic activity against B. cinerea. Not only the introduced chitinase activity but also the endogenous defense reactions activated by overexpression of CHI2 may be involved in the enhanced gray mold disease resistance in CC2. 相似文献
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二丁酰环腺苷酸对猪胴体组成和肉品质的影响 总被引:1,自引:0,他引:1
通过皮下注射和饲料添加不同剂量dbcAMP进行饲养试验 ,研究其对肥育猪胴体组成和肉品质的影响。结果表明 :皮下注射 1.0mg/kg和饲料添加 2 0mg/kg对改善肥育猪的胴体组成和肉品质效果最好 ,眼肌面积分别提高31.99%和 36 .39% (P <0 .0 5 ) ;瘦肉率分别提高 12 .4 2 %和 11.94 % (P <0 .0 5 ) ;背膘厚分别降低 19.95 %和 2 0 .97%(P <0 .0 5 )。肌肉粗蛋白含量分别提高 10 .87%和 16 .5 4 % (P <0 .0 5 )。 相似文献
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大豆黄酮对荷斯坦牛乳中蛋白质和脂肪含量的影响 总被引:8,自引:0,他引:8
22头初胎荷斯坦牛,随机分成两组。试验组于预产期前10d添喂大豆黄酮(80mg/d·头),在产后3d、10d和17d采取反射乳样品,测定其中蛋白质和脂肪含量。结果显示:添加大豆黄酮后,初乳(第3天)中的蛋白质和脂肪含量较对照组分别升高30.11%(P<0.01)和18.94%(P<0.05);常乳中的蛋白质和脂肪含量比对照组略高(P>0.05)。提示:大豆黄酮对乳成分的影响主要是提高主要营养成分蛋白质和脂肪的含量,改善初乳的品质,以提供幼畜丰富的营养 相似文献
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Prediction of chemical composition and peroxide value in unground pet foods by near‐infrared spectroscopy 下载免费PDF全文
M. De Marchi F. Righi M. Meneghesso D. Manfrin R. Ricci 《Journal of animal physiology and animal nutrition》2018,102(1):337-342
The massive development of the pet food industry in recent years has lead to the formulation of hundreds of canine and feline complete extruded foods with the objective of meeting both the needs of the animals and numerous demands from pet owners. In the meantime, highly variable raw material compositions and the industry's new production techniques oblige manufacturers to monitor all phases of the extrusion process closely in order to ensure the targeted composition and quality of the products. This study aimed at evaluating the potential of infrared technology (visible and near‐infrared spectrophotometer; 570–1842 nm) in predicting the chemical composition and peroxide value (PV) of unground commercial extruded dog foods. Six hundred and forty‐nine commercial extruded dog foods were collected. For each product, an unground aliquot was analysed by infrared instrument while a second aliquot was sent to a laboratory for proximate analysis and PV quantification. The wide range of extruded dog food typologies included in the study was responsible for the wide variability observed within each nutritional trait, especially crude fibre and ash. The mean value of the 208 pet foods sampled for PV quantification was 17.49 mEq O2/kg fat (min 2.2 and max 94.10 mEq O2/kg fat). The coefficients of determination in cross‐validation of NIRS prediction models were 0.77, 0.97, 0.83, 0.86, 0.78 and 0.94 for moisture, crude protein, crude fat, crude fibre, ash and nitrogen‐free extract (NFE) respectively. PV prediction was less precise, as demonstrated by the coefficient of determination in cross‐validation (0.66). The results demonstrated the potential of NIRS in predicting chemical composition in unground samples, with lower accuracy for moisture and ash, while PV prediction models suggest use for screening purposes only. 相似文献
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黄土高原人工灌草系统不同立地条件土壤种子库特征 总被引:2,自引:0,他引:2
本研究通过野外调查取样与室内试验相结合,连续两年对黄土高原人工灌草系统不同坡向、坡位进行取样,以期对该区土壤种子库的物种组成、密度特征及其物种多样性进行初步了解。研究结果表明:该人工灌草生态系统土壤种子库共有9科,15种,其组成因坡位、坡向以及年份而异;坡向、坡位均显著影响土壤种子库物种多样性指数、丰富度指数与土壤种子库密度(P<0.05),土壤种子库密度为3 218~5 492粒·m-2,总体呈现为阴坡显著高于阳坡(P<0.05),下坡位显著高于上坡位(P<0.05);坡位和坡向均对物种均匀性系数无显著影响;年份对土壤种子库无显著影响。上述研究可为黄土高原人工灌草系统的可持续利用与恢复提供参考依据。 相似文献