森林生态系统中土壤有机质组分的固态13C核磁共振波谱表征

Forest management practices such as prescribed burning and thinning in forest ecosystems may alter the properties of soil organic matter (SOM).In this study,surface soils from field plots in the Bankhead National Forest,Alabama,USA,were used to investigate possible SOM transformations induced by thinning and burning.Elemental analysis and solid-state 13C cross polarization magic angle spinning nuclear magnetic resonance (13C CPMAS NMR) spectroscopy were used to characterize SOM fractions in whole soils,humic substances,and density fractions.Our data revealed that the changes in SOM fractions due to the repeated burning carried out in the forest ecosystem studied were involved mainly with alkyl C,O-alkyl C,and carbohydrate functional groups,implying that most prominent reactions that occurred involved dehydrogenation,de-oxygenation,and decarboxylation.In addition,burning and thinning might have also affected the distribution and composition of free and occluded particulate SOM fractions.The limited structural changes in SOM fractions suggested that low-intensity prescribed fire in the forest ecosystem studied will not create major structural changes in SOM fractions.     

Effect of humic substances on nutrient uptake by herbage and on production and nutritive value of herbage from sown grass pastures

The effect of humic substances on the nutrient uptake, herbage production and nutritive value of herbage from sown grass pastures was studied in six field experiments. Commercial humic substances were applied in combination with mineral fertilizer or slurry, either as a solution (HF liquid; 8·3 kg humic substances ha⁻¹) or incorporated into the mineral fertilizer (HF incorporated; 3·6 to 6·4 kg humic substances ha⁻¹). A series of cuts, ranging from two to five cuts, was taken during the growing season. The general response in herbage production to application of humic substances was an increase in herbage mass of dry matter (DM) at the first cut although this was only significant in two experiments for the HF incorporated treatment. Total herbage production of DM over the growing season, however, was similar for treatments with or without application of humic substances. The overall effect of HF incorporated and HF liquid on the herbage mass of DM at the first cut across the experiments was calculated using a meta-analysis technique and it was shown that there was a significant proportional increase of 0·14 ( P  <   0·05) with the HF incorporated treatment and a non-significant increase of 0·08 with the HF liquid treatment compared to the control treatment. The nutritive value of the herbage at the first cut was similar across all treatments. In general nitrogen, phosphorus and potassium uptake at the first grass cut was higher after application of humic substances but only in one experiment was this increase statistically significant.     

Effect of fulvic and humic acids on performance, immune response and thyroid function in rats

Effect of humic acid (HA) and fulvic acid (FA) on production parameters, immune response and thyroid function of rats were investigated in two experiments. First experiment: control or 0.1%, 0.2%, 0.4%, 0.8% HA- or FA-supplemented diets. Second experiment: control and 0.4% HA- or FA-supplemented diets. The feeding period lasted 26 days in both trials. No significant changes were observed in production parameters. Ovalbumine antibody titre of rats on HA- or FA-supplemented diets showed dose-dependent (at 0.4% supplementation) and significant (p < 0.05) increase (350 and 418% respectively) over the control (100%). Dose-related increase of plasma TSH (r = 0.99), and decrease of the T(4)/T(3) ratio (r = -0.97) was observed in FA-supplemented rats. Second experiment: both FA and HA stimulated the immune response by the 14th day (mean values: control: 685.79; FA: 1131.37; HA: 1055.6099) and 26th day (control: 544.31; FA: 1969.83; HA: 1600.00). No significant differences were noted with lymphocyte stimulation test. Diameter of the 'B'-dependent lymphoid tissues in the ileum and spleen were significantly (p < 0.05) larger in both the FA- and HA-treated animals. Humic acid and FA supplementation resulted in strong humoral immune stimulation. Our data also indicate that FA content is responsible for the mild hypothyroid effect of humic substances.     

黑液制备的腐植酸液肥和调理剂的棉花田间应用研究

为了给黑液实现资源化利用提供依据,在新疆进行了黑液腐植酸产品的田间试验,设计了3个处理,采用棉花定点定株方法调查其生理指标,并测定其生化指标,研究了它们对棉花的生长、产量和抗旱能力的影响。结果表明,在滴灌中应用腐植酸液肥,与常规固体肥料相比可明显促进棉花在各生育期的生长,能增加蕾、花和铃的数量,并且延长成熟时间,作物不易早衰。同时液肥还可提高棉花的经济性状,能够显著增加籽棉的产量,增产率可达12.3%。在棉花上使用该腐植酸产品后可降低叶片的蒸腾速率,提高叶片的保水能力和CAT活力,从而明显增强棉株的抗旱能力。黑液腐植酸产品对棉花具有良好的促长、增产和抗旱效果。     

Clay dispersion and its relation to surface charge in a paddy soil of the Red River Delta,Vietnam

Dispersion is an important issue for clay leaching in soils. In paddy soils of the Red River Delta (RRD), flooding with fresh water and relatively high leaching rates can accelerate dispersion and the translocation of clay. For the clay fraction of the puddled horizon of a typical paddy soil of the RRD, the effect of various cations and anions as well as humic acid (HA) at different pH values on the surface charge (SC) were quantified and the dispersion properties were determined in test tubes and described by the C₅₀ value. In the <2 µm fraction, dominated by illite, the proportion of 2:1 vs. 1:1 clay minerals is 5:1. The organic‐C content of the clay fraction is 2.2%. Surface charge was found to be highly pH‐dependent. At pH 8 values of –32 and at pH 1 of –8 mmol_c kg^–1 were obtained. Complete dispersion was observed at pH > 4, where SC is > –18 mmol_c kg^–1. The flocculation efficiency of Ca strongly depends on the pH. At pH 4, the C₅₀ value is 0.33, 0.66 at pH 5, and 0.90 mmol L^–1 at pH 6. At pH 6, close to realistic conditions of paddy soils, the effect of divalent cations on the SC and flocculation decreases in the order: Pb > Cu > Cd > Fe^II > Zn > Ca > Mn^II > Mg; Fe^II was found to have a slightly stronger effect on flocculation than Ca. An increase in concentrations of Ca, Mn^II, and Mg from 0 to 1 mmol L^–1 resulted in a change in SC from –25 to approx. –15 mmol_c kg^–1. In comparison, the divalent heavy‐metal cations Pb, Cu, Cd, and Zn were found to neutralize the SC more effectively. At a Pb concentration of 1 mmol L^–1, the SC is –2 mmol_c kg^–1. From pH 3 to 5, the dispersion of the clay fraction is facilitated rather by SO than by Cl^–, which can be explained by the higher affinity of SO to the positively charged sites. With an increase of the amount of HA added, the SC continuously shifts to more negative values, and higher concentrations of cations are needed for flocculation. At pH 3, where flocculation is usually observed, the presence of HA at a concentration of 40 mg L^–1 resulted in a dispersion of the clay fraction. While high anion concentrations and the presence of HA counteract flocculation by making the SC more negative, Fe^II and Ca (C₅₀ at pH 6 = 0.8 and 0.9 mmol L^–1, respectively) are the main factors for the flocculation of the clay fraction. For Fe^II and Ca, the most common cations in soil solution, the C₅₀ values were found to be relatively close together at pH 4, 5, and 6, respectively. Depending on the specific mineralogical composition of the clay fraction, SC is a suitable measure for the determination of dispersion properties and for the development of methods to keep clay particles in the soil in the flocculated state.     

Soil Organic Carbon and Nitrogen Stocks under Tropical Organic and Conventional Cropping Systems in Northeastern Brazil

Soil organic matter (SOM) is an essential ecosystem component whose dynamics are affected by soil management practices. To evaluate the impact of two agricultural systems (organic and conventional) on soil organic carbon (C) and nitrogen (N) stocks in a sandy soil, samples were collected from the Amway Nutrilite Brazil farm and from the Central Pivot Horticultural Farmers Association farm, both situated in the Chapada da Ibiapaba region, Ceará State, Brazil. The first area has a large‐scale Caribbean Cherry fruit production system under organic management, whereas the second represented a conventional soil cultivation condition, characterized by the use of chisel plow and disc plow, mineral fertilization, and herbicides application. Plots with and without green manure fertilization were compared in the organically cultivated systems by using soil samples collected in the rows and between the rows. Areas under native forest were also sampled to determine the steady‐state condition. Total organic C and N contents in the soil (SOC, TN) and in the humic substances were determined at the 0‐ to 5‐, 5‐ to 15‐, 15‐ to 30‐, and 30‐ to 50‐cm soil layers. In addition, oxidizible organic C fractions were measured to calculate the carbon management index (CMI). In general, total SOC levels were low, ranging from 2.5 to 12.6 g kg^?1 in the whole soil profile among the organic systems. In the upper soil layer, SOC and TN stocks were greater in the rows in response to organic fertilization. The conventional system presented lower variation on the SOC contents throughout soil layers when compared to the native forest area, indicating the direct effect of plowing on the downward SOC distribution. The CMI data confirmed the reestablishment of SOM levels in the rows of the organic managed systems in relation to the reference area, whereas the reduction of the CMI in the conventional system suggests a decline in the soil quality and greater potential for increased C losses to the atmosphere.     

Effects of Exogenous Humic Acids on Forms of Organic Phosphorus in Three Contrasting Types of Soil

A laboratory incubation study was conducted to examine the effects of exogenous humic acids on the forms of organic phosphorus (P) in three contrasting soil types: red soil, brown soil, and drab soil. Results indicated that the Olsen P concentration increased in all the three types of soil with addition of the humic acid extracts, with the magnitude of increment ranked in a sequence of brown soil > drab soil > red soil. For the soil organic P fractions, addition of the humic acid extracts increased the labile organic P and moderately labile organic P concentrations while reducing the moderately resistant organic P and highly resistant organic P concentrations, suggesting that the exogenous humic acids could enhance transformation of organic P from resistant forms to labile forms. The activation of organic P by addition of the humic acid extracts varied with soil type, and this variation was related to soil characteristics.     

Physico‐chemical characterization of humic‐metal‐phosphate complexes and their potential application to the manufacture of new types of phosphate‐based fertilizers

The aim of this review is to describe the main physicochemical characteristics of diverse types of humic‐metal‐phosphate acid complexes. The effects of these complexes on phosphorus (P) fixation in soils with different pH values and physicochemical features and on plant phosphorus uptake are also discussed. Humic‐metal‐phosphate complexes have apparent stability constants in the same range as those of metal‐humic complexes, in solutions with diverse pH and ionic‐strength values. Likewise, the molecular‐size distribution of humic‐metal‐phosphate complexes as a function of pH is similar to that of potassium or sodium humates and metal‐humic complexes. Humic‐metal‐phosphate complexes are able to decrease phosphate fixation in soils and increase plant growth and phosphate uptake. Phosphorus fertilizers containing humic‐metal‐phosphate complexes proved to be efficient to improve plant growth and P uptake with respect to conventional fertilizers such as single superphosphate. The values of parameters related to plant phosphorus‐utilization efficiency (PUt E) suggest that the regulation of root acquisition of phosphate from these complexes could involve the interregulation of a system for the optimization of metabolic P utilization in the shoot and another system involving stress responses of roots under phosphorus deficiency.     

腐殖酸与水稻抗冷性的研究初探

低温胁迫下，腐殖酸可提高水稻脯氨酸含量，多酚氧化酶比活力，脱落酸含量，并减少了水稻的质膜诱性及丙二醛含量。试验证明，腐殖酸确实可以提高水稻的抗冷性     

生物质垃圾转化为生态肥料的水热技术试验研究

在2L的高压反应釜中，利用水热技术，对甘蔗渣、树叶和菜皮3种生物质垃圾的转化产物和机理进行了研究。结果表明，在反应温度为140℃～260℃、反应时间为1～4h、催化剂为Na2CO3的条件下，生物质转化得到的产物中含有大量的腐植酸物质。此外，研究了温度、时间和催化剂等条件对产物中腐植酸物质的含量的影响，并对产物中的含氧基团进行了测定。通过比较发现，在温度为160℃、时间为1．5h的条件下，固态产物中的腐植酸含量能达到45％左右。这类腐植酸物质可以作为生态肥料，具有良好的肥效和经济价值。