重金属在小麦作物体中残留特征的田间试验研究

研究表明经污水灌溉后重金属铅、砷和镉在小麦植物体中的残留量因部位不同而有差异,但清水和污水灌溉对其总体残留特征无明显影响。其总的分布趋势是小麦根部的重金属累积量最大，其次是小麦的茎叶部，再其次是小麦的穗部，即小麦植株体内重金属积累量由大到小依次为根＞茎叶＞穗。因此认为根可作为一种屏障使重金属铅、砷和镉难于向上部迁移，使地上部分免受其害。     

某硫矿重金属分级及土柱淋溶

Fractions of various heavy metals in a sulfidic minespoil were investigated.Column leachine experiment was also conducted to simulate “acid mine drainage“(AMD) from the minespoil.The results show that leaching of heavy metals from the minespoil was extremely significant during the initial water flushing.The amounts of heavy metals leached out dramatically reduced after leaching twice.It is worthwhile to note that in this study,Zn,Mn,Fe,As and Ni in the first leachate exceeded the total amount of each corresponding water-extractable(1:5,soil:water)metal contained in the minespoil sample.This appears to suggest that 1:5 water extraction did not allow accurate estimation of water-leachable concentrations of the above heavy metals.This work has implications for the management of sulfidic minespolis.Acid drainage of great environmental concerns is likely to occur only during heavy rainfall events after substantial soluble and readily exchangeable acid and metals are accumulated in the minespoils.The slow-reacting fractions other than water-soulble and readily exchangeable fractions may pose little environmental hazards.This is particularly true for Pb,As and Ni.     

天津市不同污染梯度树木吸滞重金属及硫能力分析

以天津市主要绿化树种为研究对象,对叶片中重金属元素和硫含量进行了分析。结果表明:各研究区域内重金属污染程度为:昆仑桥(市区)水上公园(近郊公园区)蓟县(远郊园林区)杨柳青庄园(远郊风景区),随着市中心到远郊污染程度降低,不同区域各树种叶片吸滞重金属程度也随之降低,4个区域树木叶片吸滞污染元素排序大致均为:SAsPbCuCrCd;在昆仑桥柳树对各元素的吸滞能力均比较强,槐树吸滞As和Cr能力较强,杨树吸滞Pb和Cr能力较强,白蜡吸滞Cu和As能力较强;水上公园桧柏吸滞Cd和Cr能力较强,龙柏吸滞Cd和As能力较强,白蜡对Pb和Cu吸滞能力较强,柳树对S吸滞能力较强,雪松对各种元素的吸滞能力较强,杨树吸滞各元素能力相对较弱;杨柳青庄园桧柏吸滞Cr能力较强,雪松和龙柏吸滞Pb能力较强,杨树对Cd和Cu吸滞能力较强,杜仲对As和S吸滞能力较强;蓟县雪松吸滞Cr的能力较强,杨树吸滞Cd,S和Cu的能力较强,柳树对各元素的吸滞能力均较强,龙柏对各元素吸滞能力均相对较弱。     

乳酸菌复合系和植物乳杆菌提高柳枝稷青贮效果

该文旨在探讨接种乳酸菌复合系SGL和植物乳杆菌（Lactobacillus plantarum）对柳枝稷青贮效果的影响。以营养生长阶段的柳枝稷为原料,分别接种1 OD600/kg 鲜质量的SGL和Lactobacillus Plantarum,以不接菌的处理作为对照。在发酵第3、10、20和30天开罐取样进行检测分析发酵饲料品质,通过短期人工瘤胃技术测定体外瘤胃发酵参数的变化,并运用高通量测序技术分析原料和青贮料的细菌多样性。结果表明：接种乳酸菌复合系SGL和Lactobacillus Plantarum均能有效抑制Enterobacter和Clostridium,使Lactobacillus成为控制发酵的优势菌,加快青贮过程中pH值的下降速率,提高柳枝稷青贮饲料品质,并提高柳枝稷青贮料的瘤胃发酵效果,复合菌系SGL比纯培养的Lactobacillus Plantarum的效果更突出。     

Long-term impact of fertilization on activity and composition of bacterial communities and metabolic guilds in agricultural soil

To explore long-term impact of organic and inorganic fertilizers on microbial communities, we targeted both the total bacterial community and the autotrophic ammonia oxidizing bacteria (AOB) in soil from six treatments at an experimental field site established in 1956: cattle manure, sewage sludge, Ca(NO₃)₂, (NH₄)₂SO₄, unfertilized and unfertilized without crops. All plots, except the bare fallows, were cropped with maize. Effects on activity were assessed by measuring the basal respiration and substrate induced respiration (SIR) rates, and the potential activity of the AOB. To determine the bacterial community composition, 16S rRNA genes were used to fingerprint total soil communities by terminal restriction fragment length polymorphism analysis and AOB communities by denaturing gradient gel electrophoresis. The fertilization regimes had clear effects on both activity and composition of the soil communities. Basal respiration and r, which was kinetically derived as the exponentially growing fraction of the SIR-response, correlated well with the soil organic C content (r=0.93 and 0.66, respectively). Soil pH ranged from 3.97 to 6.26 in the treatments and was found to be an important factor influencing all microbial activities. pH correlated negatively with the ratio between basal respiration and SIR (r=0.90), indicating a decreased efficiency of heterotrophic microorganisms to convert organic carbon into microbial biomass in the most acid soils with pH 3.97 and 4.68 ((NH₄)₂SO₄ and sewage sludge fertilized plots, respectively). The lowest SIR and ammonia oxidation rates were also found in these treatments. In addition, these treatments exhibited individually different community fingerprints, showing that pH affected the composition of AOB and total bacterial communities. The manure fertilized plots harbored the most diverse AOB community and the pattern was linked to a high potential ammonia oxidation activity. Thus, the AOB community composition appeared to be more strongly linked to the activity than the total bacterial communities were, likely explained by physiological differences in the populations present.     

Multi-element analysis of soils and sediments by wavelength-dispersive X-ray fluorescence spectrometry

Soil and sediment reference materials were used to calibrate and evaluate an analytical method for the determination of major (Si, Al, Fe, Mg, Ca, Na, K, Mn, P, Ti) and trace elements (As, Ba, Cd, Co, Cr, Cu, Ga, Mo, Mb, Ni, Pb, Rb, S, Sb, Sn, Sr, Th, U, V, Y, Zn, Zr) by sequential wavelength X-ray fluorescence spectrometry. Samples were prepared as pressed pellets and analysis was done with a total measuring time of thirty minutes per sample. Special attention was given to the selection of the thirty reference materials used for calibration of the spectrometer. Another set of eleven RM (reference materials) was analyzed for the evaluation of accuracy. Detection limits for trace elements (1-2 mg kg^-1) are adequate both for geochemical and environmental purposes, except for Cd and Sb. Accuracy for trace elements falls within the expected interval of certified or recommended values in most cases, but for some major elements, like SiO₂, some results showed discrepancies, evidencing difficulties associated with the determination of light elements in complex matrices. But when quality criteria proposed by mapping programs are applied to the results, their requirements are fulfilled. Both instrumental precision, obtained by twelve sample replicate analyses, and analytical precision, considering also sub-sampling and pellet preparation, lie between the limits of the Horwitz expression, except at concentrations close to the detection limits.     

Nitrogen transformations in copper-contaminated soils and effects of lime and compost application on soil resiliency

 A neutral and an acidic soil were treated with different doses (0–3,000 mg Cu kg^–1 soil as CuSO₄) of copper. The percentages of inhibition of nitrification in both soils varied from 5 to 97%, but for the N mineralization these percentages varied from 8 to 65%. The toxic effect of Cu for basal nitrification and N mineralization was assessed as critical. Nitrification was more sensitive than ammonification to copper toxicity. It appears that an ecological dose of inhibition for nitrification and N mineralization higher than 10% is suitable as an indicator for Cu contamination. Soil resiliency assessed by N mineralization in the lime treatments varied from 11 to 154% in the sandy soil and from 70 to 168% in the sandy loam soil. A combined application of lime and compost significantly increased soil resiliency. The percentage increase varied from 904 to 1,390% in the sandy soil and from 767 to 2,230% in the sandy loam soil. It appears that compost was a powerful agent for recovering the soil fertility of Cu-contaminated soils as assessed by N transformation. The acidic sandy soil showed a lower capacity for recovery after Cu toxicity stress. Received: 27 February 1999     

光合细菌对罗非鱼塘中水质和生态的影响

研究了在罗非鱼池塘养殖过程中投以光合细菌后，对池塘水质及水中微生物种群数量的变化影响，通过2个试验组和2个对照组进行为期半个月的试验。试验结果表明：（1）投放光合细菌的池塘中，光合细菌种群由少到多不断地增长，并且形成优势种群；（2）投放光合细菌后，对降低水中COD、氨氮，提高亚硝酸态氮等有一定的效果；（3）但是对水中溶氧、pH值、硝酸态氮等的变化并没有起太大的作用。     

Direct and Residual Effects of Fly Ash-Based Integrated Fertilization on Rice (Oryza sativa L.) and Rapeseed (Brassica camprestris L.)

Integrated effects of fly ash (FA), farmyard manure (FYM), and chemical fertilizers recommended dose of fertilizer (RDF) on the performance of rice (Oryza sativa L.) and their residual effect on rapeseed (Brassica camprestris L. vartoria) were studied for two years in loamy acidic inceptisol of Assam, India.

The FA was generated from a bituminous and lignite coal-based captive thermal power plant of Hindustan Paper Corporation Ltd (HPCL), Assam. Rice yields were higher when FA, FYM, and RDF were used collectively vis-à-vis sole application of RDF reflected also in rapeseed under residual effect . FA effect on mean rice equivalent yield of the rice–rapeseed system was the highest (24.4%) under integrated application. Nickel (Ni) and cadmium (Cd) contents in rice decreased with increasing FA, while arsenic (As) was just the opposite. Integration of FA, FYM, and RDF was effective in lowering Ni, Cd, and As in rice compared to 100% RDF alone or together with FA. The residual effect on rapeseed was similar for Ni and As while the Cd content increased. Blending of FA, FYM, and RDF also positively altered the residual soil pH, organic carbon (C), and nitrogen, phosphorus, and potassium (NPK). Thus, FA can be an integral component of integrated plant nutrition system (IPNS) in augmenting crop yield and residual benefits in loamy acid inceptisol.     

用稀悬液Wien效应测量法来研究重金属离子与水稻土的相互作用

Interactions of three heavy metal ions, Cu²⁺, Cd²⁺, and Pb²⁺, and, for comparison, Na⁺ with electrodialytic clay fractions (less than 2 μm in diameter) of four paddy soils as well as a yellow-brown soil as a control soil were evaluated based on measurements of the Wien effect in dilute suspensions with a clay concentration of 10 g kg^?1 in four nitrate solutions of 2 × 10^?4/z mol L^?1, where z is the cation valence, and a nitric acid solution of 3 × 10^?5 mol L^?1. Field strengths ranging from 15 to 230 kV cm^?1 were applied for measuring the electrical conductivities (ECs) of the suspensions. The mean free binding energies between the various cations and all of the soils determined from exchange equilibrium increased in the order: Na⁺ < Cd²⁺ < Cu²⁺ < Pb²⁺. In general, the ECs of the suspensions in the sodium nitrate solution were smaller than those of the suspensions in the heavy metal solutions because of the lower electrophoretic mobility of sodium compared to the divalent cations. In terms of relative electrical conductivity-field strength relationships, relative electrical conductivity (REC) of suspensions containing various cations at field strengths larger than about 50 kV cm^?1 were in the descending order: Na⁺ > Cu²⁺ > Cd²⁺ > Pb²⁺ for all tested soils. A characteristic parameter of the REC-field strength curves, ΔREC₂₀₀, REC at a field strength of 200 kV cm^?1 minus that at the local minimum of the concave segment of the REC-field strength curves, characterized the strength of adsorption of the cations stripped off by the applied strong electrical field, and for all soils the values of ΔREC₂₀₀ were generally in the order: Na⁺ < Cu²⁺ ≤ Cd²⁺ ≤ Pb²⁺.