Resistance to Leveillula taurica in the genus Capsicum

One hundred and sixty-two Capsicum genotypes were evaluated for powdery mildew (Leveillula taurica) resistance, following inoculations with a suspension of 5 × 10⁴ conidia mL⁻¹ on 10-leaved to 12-leaved plants. Genotypes were graded into five resistance classes, based on the areas under the disease progress curves calculated from disease incidence (percentage infected leaves per plant) and severity (total number of colonies per plant). Results revealed a continuum from resistance to susceptibility, with the majority (70%) of C. annuum materials being classified as moderately to highly susceptible to L. taurica. Conversely, C. baccatum, C. chinense and C. frutescens were most often resistant, indicating that resistance to L. taurica among Capsicum species is found mainly outside the C. annuum taxon. Nevertheless, some resistant C. annuum material was identified that may be useful for resistance breeding. Eight genotypes were identified as immune to the pathogen: H-V-12 and 4638 (previously reported), and CNPH 36, 38, 50, 52, 279 and 288. Only H-V-12 and 4638 are C. annuum, while all others belong to the C. baccatum taxon. Latent period of disease on a set of commercial sweet pepper genotypes varied, indicating diverse levels of polygenic resistance. The latent period progressively reduced with plant maturity, from 14·3 days in plants at the mid-vegetative stage to 8·6 days in plants at the fruiting stage. Young plants of all commercial genotypes tested at the early vegetative stage were immune, irrespective of the reaction of the genotype at later stages, demonstrating widespread juvenile resistance to L. taurica in the Capsicum germplasm. Inoculation of plants of different botanical taxa with a local isolate indicated a wide host range. Some hosts, including tomato (Lycopersicon esculentum), artichoke (Cynara scolymus) and poinsettia (Euphorbia pulcherrima), produced large amounts of secondary inoculum. Other hosts included okra (Abelmoschus esculentus), eggplant (Solanum melongena), cucumber (Cucumis sativus), Solanum gilo, Chenopodium ambrosioides and Nicandra physaloides.     

甘肃陇南小麦不同品种类型抗条锈性变化特点分析

 1973~2002年的30年间,在我国小麦条锈病常发易变区的陇南,对小麦主要生产品种的抗条锈性变化动态进行了系统的观察,选择具垂直抗性、慢条锈性、高温抗性和持久抗性等不同类型的7个品种的观测数据进行了分析,认为里勃留拉和N.斯特拉姆潘列长期保持了稳定的抗锈性。里勃留拉的抗性可能由多对微效基因控制,N.斯特拉姆潘列的抗性可能由主效基因控制,兰天12号所具有的慢锈性和兰天1号的高温抗性以及抗条锈基因的合理布局对延长品种抗性也有一定作用。     

A coat protein transgene from a Scottish isolate of potato mop-top virus mediates strong resistance against Scandinavian isolates which have similar coat protein genes

Resistance tests were made on seedlings of transformed lines of Nicotiana benthamiana which contain a transgene encoding the coat protein (CP) gene of a Scottish isolate of potato mop-top virus (PMTV). This transgene has been reported to confer strong resistance to the PMTV isolate from which the transgene sequence was derived and also to a second Scottish isolate. Plants of lines of the transgenic N. benthamiana were as resistant to two Swedish and two Danish PMTV isolates as to a Scottish isolate, and of five lines tested, greater than 93.5% of transgenic plants were immune. The coat protein gene sequences of these four Scandinavian isolates were very similar to those of the two Scottish isolates. The greatest divergence between the isolates was three amino acid changes and there was less than 2% change in CP gene nucleotide sequence. It is concluded that the PMTV CP transgene used in these experiments could confer resistance against isolates from different geographical areas because it is becoming apparent that the CP genes of PMTV isolates are highly conserved.     

粳稻品种抗稻瘟病性与其生物学性状的相关关系研究

对３４２份粳稻材料进行抗稻瘟病性（抗叶瘟、穗颈瘟）与其生物学性状（株高、穗长、每穗粒数、分蘖率、单株有效分蘖数、空秕率、着粒密度，以及成熟期等）相关关系研究，结果不同品种（系）间存在着一定差异。抗叶瘟性与株高、穗长、每穗粒数、单株有效分蘖数、着粒密度呈显著正相关，相关系数依次为：０．４０９、０．４９１、０．４２６、０．６５７、０．４７１；与空秕率呈显著负相关，相关系数为－０．５９１。抗穗颈瘟性与株高、每穗粒数、单株有效分蘖数呈显著正相关，相关系数分别为：０．７１７、０．４６２、０．３６２；与空秕率、分蘖率、着粒密度呈显著负相关，相关系数依次为：－０．３４３、－０．３３２、－０．４７６。试验表明，株高、单株有效分蘖率、每穗粒数、空秕率等性状，可做为主要标记，在粳稻品种（系）抗稻瘟病性选鉴上应用     

非自然越夏区小麦白粉病模拟鉴定病圃

作者于1990～1995年根据陕西省关中麦区小麦白粉病侵染循环特点,人工模拟自然发病条件,在非自然越夏区杨陵(省农科院试验地)成功地建立起白粉病菌可周年存活,且秋、春季均能充分诱发感染的混合菌系病圃,宜对小麦种质材料进行全生育期抗白粉病鉴定。利用该病圃已对陕西省1000余份小麦品种(系)及部分国内外抗源亲本材料做了抗病性鉴定和利用评价。     

棉铃虫对拟除虫菊酯类杀虫剂抗性遗传力的分析

采用域性状分析法,估算了棉铃虫对氰戊菊酯、三氟氯氰菊酯和溴氟菊酯的抗性现实遗传力,并对3种菊酯的抗性风险进行了评估。结果表明,棉铃虫对氰戊菊酯、三氟氯氰菊酯和溴氟菊酯的抗性现实遗传力分别为0.2027、0.1554和0.1084。假设田间种群的抗性现实遗传力为估算值的一半,杀死率为80％,预计抗性增长到10倍时,氰戊菊酯可使用14次,三氟氯氰菊酯可使用19次,溴氟菊酯可使用31次。三氟氯氰菊酯和溴氟菊酯的抗性风险明显低于氰戊菊酯;棉铃虫对含氟菊酯的抗性发展速度明显低于不含氟的菊酯。试验结果为实际应用提供了理论依据。     

Neurospora crassa对三唑醇的抗药性分子机制研究

 通过紫外光诱变获得Neurospora crassa对三唑醇杀菌剂抗性突变体"Nc70"。用2.5μg/ml三唑醇处理,突变体内甲基甾醇与脱甲基甾醇比例变化幅度远远小于亲本敏感菌株"STA"。突变体对三唑醇的吸收能力与敏感菌株相似,但药剂在体细胞内积累较少。三唑醇处理N.crassa 2 h后,药剂在菌体内的含量趋向稳定,突变体内的药量较敏感菌体低56.1%,而麦角甾醇合成量则高于敏感菌株53.7%。     

奶牛中草药抗热应激添加剂剂量的对比试验

40头荷斯坦泌乳牛随机分成4组,采用单因子试验设计,分别饲喂由基础日粮与不同剂量的中草药添加剂(每头·日0g为A组即对照组、25g为B组、75g为C组、125g为D组)组成的日粮。结果表明:干物质摄入量C、D组分别比对照组多摄入1.6kg、1.6kg。校正体重和乳脂的产乳量,C组、D组分别较对照组提高46.3%(P<0.01)和34.4%(P<0.01),且C组、D组乳脂率较对照组分别提高8.8%(P<0.05)、20%(P<0.05)。C组、D组红细胞数较对照组分别提高35.8%(P<0.01)、33.9%(P<0.01),而呼吸率则分别降低43.3次/分、47.2次/分、肛温分别降低1.2℃、1.1℃。对照组中暑死亡两头,其余组没有死亡。经济效益,C组、D组分别比对照组提高83.7%.、51.2%。     

肉仔鸡生长过程中行为与产热的变化

研究肉仔鸡成长过程中行为变化对产热量的影响 ,将 2日龄肉仔鸡 36只分为自由摄食、 50 %摄食和绝食 3组进行观测。立位行为集中在明期 ,特别是摄食 30min前后 ,暗期几乎没有出现立体行为。 2日龄时 ,每天约有 70 0min是立位的 ,其后 ,随着日龄的增加而减少。在自由采食区 ,公雏比母雏摄食时间长。 50 %摄食区较自由采食区的摄食时间少。在绝食区 ,随着日龄的增加 ,公母的立位时间呈现减少倾向。 2 1日龄之前随着日龄的增加其摄食时间也增加 ,其后呈现相反倾向。产热 (HP ,kJ/kg0 75)与摄食时间有相同倾向。在 2 1日龄前公雏的HP比母雏低 ,2 1日龄后母雏比公雏低。 1天中各个区的HP活动规律是摄食后 1h内最高 ,暗期降低 ;限制摄食量后 ,HP降低 ,明期和暗期的HP差也随之减小