生长激素释放肽-6缓释微球的制备及其对动物生长的影响

以生物降解材料乳酸乙醇酸共聚物(PLGA,LA-GA 8515)为载体,采用复乳-液中蒸发法制备含生长激素释放肽-6的乳酸乙醇酸共聚物微球,并通过体外药物释放试验评价载药微球的释药特征.结果得到收率、粒径大小和分布及含药量均满意的微球,其中包封率为100%,平均体积径为3.45μm,收率为79%.在37℃、pH7.0的生理等渗缓冲液中,首日释药19.19%,其后以平均日释药3.23%的速度释药25 d.小鼠肌肉注射微球30 d后,累积增重比注射生长激素释放肽-6、生理盐水分别高22.56%(P<0.05)和53.17%(P<0.01).结果表明,生长激素释放肽-6乳酸乙醇酸共聚物微球有望发展成为新型长效制剂.     

微卫星与金华猪生长性状关系的研究

采用微卫星标记多重扩增结合荧光标记检测技术,对152头金华猪种群的30个微卫星位点的不同基因型与生长发育性状的关系进行了初步分析和显著性检测。结果显示SW 2476、SW 0215、SW 24等3个微卫星位点不同基因型间的出生重差异显著;SW 902、S0143、SW 0215、S0228、SW 24等5个微卫星位点不同基因型间的60日龄重差异显著;S0178、S0218、S0255、S0155、SW 951等5个微卫星位点不同基因型的4月龄重差异显著;而S0217微卫星位点的不同基因型的6月龄重差异显著。     

Biochemical proximates of pumpkin (Cucurbitaeae spp.) and their beneficial effects on the general well‐being of poultry species

There is a growing need to increase productivity in poultry. Growth hormones and antibiotics have the ability to improve health, weight gain and feed efficiency in meat‐producing animals. The growth‐promoting antibiotics are administered to poultry to improve the general performance of the chicken. However, the use of the xenobiotic drugs in food‐producing animals has been a concern and a sensitive issue of debate for several decades in the EU and many other regional blocks of the world. Consequently, the use of hormones in animal production has been banned in Italy, Denmark and Germany for over 4–5 decades, while Belgium and Greece had never permitted its use for livestock fattening purposes. Bioactive phytochemicals exhibit antimicrobial, antioxidant, antiparasitic, antiprotozoal, antifungal and anti‐inflammatory properties and consequently have several beneficial effects on appetite, growth and the immune status of the animal. In South Africa, different species of pumpkin are produced for food due to their high nutrient content. The flesh serves as a traditional food, while the seeds and peels are commonly discarded. Pumpkin seed extract is reported to be useful for immunomodulation, reproductive health, therapeutics over a wide range of disease conditions and stimulates metabolism of accumulated fats. Studies have also shown that pumpkin seeds are a valuable source of protein and fat. Their complexity and extent of bioactivity offers sustainable prospects for natural control of pathogenic/parasitic organisms, stimulate nutrition or enhance resistance to disease infections, and reduce abdominal fat and serum levels of harmful lipids, while increasing serum levels of beneficial lipids.     

豆科与禾本科牧草间作的生长互作效应及对氮、磷养分吸收的影响

为了解牧草间作对作物生长和养分吸收的影响,以及为不同牧草优化配置、豆科和禾本科牧草间作提高结氮量提供理论依据,本试验采用随机区组试验设计,研究了间作对柱花草、扭黄茅和孔颖草的生物量和氮磷吸收的影响。结果表明,由于柱花草生物固氮,对扭黄茅和孔颖草养分吸收和生长有促进作用,与柱花草间作后的扭黄茅和孔颖草与禾本科牧草单作模式相比,能获得高的养分吸收量和产量。与各自单作对比,间作体系中的禾本科牧草生物量高于单作0.06%和26.78%;与柱花草单作对比,两个间作体系中的柱花草生物量低于单作9.70%和12.83%。扭黄茅与柱花草间作后,对氮磷的吸收量高于单作扭黄茅24.26%和35.18%,对氮磷的吸收量高于单作孔颖草40.64%和47.50%。相对于单作而言,柱花草分别与扭黄茅和孔颖草间作,对氮吸收量分别降低8.31%和28.94%,磷吸收量分别降低3.56%和20.58%。而且两种间作模式当量比均大于1,具有间作优势;柱花草/扭黄茅间作系统生产力、种间竞争能力高于柱花草/孔颖草,具有产量优势。在株行距为50 cm种植密度下,间作体系中禾本科牧草生长旺盛,柱花草的生长受到抑制,在与两个禾本科牧草间作中为弱竞争作物。结果表明,在合理的种植密度下,适合的牧草配置能够充分利用各种资源,促进种间有益作用,提高单位面积牧草产量和禾本科牧草氮磷的吸收,有利于改善牧草品质。     

草地群落类型对乳白香青种群特征和雌雄株形态及抗性的影响

为探究亚高山雌雄异株草本植物乳白香青对生境的适应方式,通过测定5个草地群落(A-老芒麦改良草地,B-虉草改良草地,C-天然草地,D-天然草甸,E-沙化草地)中乳白香青种群数、种群密度和面积、分布系数、性比组成,以及雌雄株构件性状、生物量配比、水分分配及抗性物质含量等参数,研究群落组成对乳白香青种群特征、雌雄株形态可塑性及抗性的影响。结果表明,5个草地群落的优势种、群落密度、基盖度、物种密度和优势种高度及土壤理化性状的显著差异(P<0.05),使不同群落中乳白香青种群特征显著不同,群落类型对种群特征参数影响顺序为种群面积>种群密度>性比>年龄结构>种群数>分布系数;D群落中乳白香青种群数和分布系数最高,C中乳白香青种群面积和密度最大,E中乳白香青种群数仅分别为D和C中的30.21%和35.11%,分布系数仅为C中的36.04%;群落类型对乳白香青种群年龄结构和性比有显著影响(P<0.05),雌株数为雄株的2.22~7.03倍;叶数和叶面积、株高和茎径、根长、小花数和苞片层数有显著的性别差异(P<0.05),群落类型对雌雄株的叶数和叶面积、株高、根数、花序数和小花数有显著影响,对雄株茎径和苞片层数有显著影响;雄株总生物量和花分配比显著高于雌株,而根分配比显著低于雌株(P<0.05),群落类型对乳白香青生物量配比有显著影响,雌株生物量配比受影响顺序为花>总生物量>叶>根>茎,雄株受影响顺序为花>茎>叶>总生物量>根;雄株茎、叶、花含水量显著高于雌株(P<0.05),根含水量在性别间和群落间差异较小,雌株构件含水量受群落类型影响顺序为花>茎>叶>根,雄株为叶>茎>花>根。雄株中MDA含量高于雌株,雌株中H₂O₂含量及T-AOC活性大于雄株(P<0.05),群落组成对雌、雄株中H₂O₂和MDA含量及T-AOC活性有极显著影响(P<0.01)。A和B中H₂O₂和MDA含量最低,E中H₂O₂和MDA达最大值,而T-AOC活性最低。雌、雄个体均能依据群落生境调整生长策略,通过构件性状、生物量配比、水分分配和抗性生理等适应性变化,使种群具有与生境匹配的种群特征,利于提高种群适合度和生活史完成。     

断奶前补饲不同直/支链淀粉比开食料对羔羊瘤胃上皮发育的影响

为了研究断奶前补饲不同直/支链淀粉比开食料对羔羊瘤胃上皮发育的影响,选取24只体况良好、胎次一致、体重相近的10日龄羔羊(湖羊),将其随机分为3组,在饲喂相同母乳的基础上,分别补饲以纯木薯(CS)、玉米(MS)和豌豆淀粉(PS)(直/支链淀粉比分别约为0.11,0.27和0.44)为唯一淀粉来源的开食料。试验期间,每天4:00-19:00将羔羊抱入补饲栏补饲不同直/支链淀粉比的开食料,并且在6:30, 10:30和15:30将羔羊抱回母羊舍哺乳1 h。羔羊自由饮水,单栏饲喂,自由采食燕麦干草。56日龄时屠宰采样,采集瘤胃组织样品制作石蜡切片进行瘤胃乳头形态测定,采集瘤胃上皮样品提取RNA测定相关基因表达。结果表明:CS组羔羊瘤胃乳头长度和表面积显著(P<0.001)高于MS和PS组,CS和MS组羔羊瘤胃乳头宽度显著(P=0.001)高于PS组。对瘤胃上皮各层厚度统计显示,CS和PS组羔羊瘤胃上皮角质层厚度显著(P=0.001)高于MS组;CS和MS组羔羊瘤胃上皮颗粒层厚度显著高于(P<0.001)PS组;CS组羔羊瘤胃上皮棘基层厚度和总厚度显著(P<0.001)高于MS和PS组。qRT-PCR结果显示:不同直/支链淀粉比开食料显著影响羔羊瘤胃上皮CDK2和CDK6的mRNA表达量(CS>MS>PS, P<0.001);CS组羔羊瘤胃上皮细胞cyclin A和CDK4的mRNA表达量显著(P<0.05)高于PS组,但与MS组无显著差异;CS组羔羊瘤胃上皮cyclin D1的mRNA表达量显著(P=0.012)低于PS组,但与MS组无显著差异。CS组羔羊瘤胃上皮类胰岛素生长因子-1(insulin like growth factor,IGF-1)的mRNA表达量显著(P<0.001)高于MS和PS组,CS和MS组羔羊瘤胃上皮IGF-1R的mRNA表达量显著(P=0.001)高于PS组。上述结果表明:较高支链淀粉比开食料与较高直链淀粉比开食料相比,在瘤胃中较易降解生成挥发性脂肪酸,促进瘤胃上皮IGF-1及细胞周期蛋白mRNA的表达,进而有利于断奶前羔羊瘤胃上皮的发育。研究结果对制订羔羊营养方案具有重要指导意义。     

开食料中不同NDF水平对犊牛生长性能、瘤胃内环境及血清生化指标的影响

为探索开食料中不同NDF水平对犊牛生长、瘤胃发酵参数以及血清生化指标的影响,选用初生重相近(42±2.5) kg、饲喂足量初乳的中国荷斯坦犊牛60头,其中公犊牛36头,母犊牛24头。随机分为A、B、C和D 4个处理组,每个处理15头,其中9头公犊牛+6头母犊牛,试验犊牛于犊牛岛(1.5 m×3.4 m)单独饲养。15日龄开始分别饲喂NDF水平不同的4种开食料,分别为:A处理10%,B处理15%,C处理20%和D处理25%,70日龄断奶,试验期112 d。结果表明:1)0~42日龄和70~112日龄,B组日增重均高于A、C和D组(P<0.05),分别高出45.69、39.79、117.13 g·d^-1和35.33、153.29、 145.93 g·d^-1。70~112日龄,B、C和D组开食料采食量显著高于A组(P<0.05),分别高出255.20、252.48和392.27 g·d^-1。A(0.61)和B(0.60)组犊牛的饲料转化率显著高于C(0.55)和D(0.54)组(P<0.05);2)犊牛瘤胃pH在35和70日龄时,C组显著高于其他3组(P<0.05),112日龄时,A和B组显著高于C和D组(P<0.05);3)瘤胃NH₃-N含量在90日龄前差异不显著(P>0.05),但在112日龄时, B、C和D组较A组显著降低16.72%、44.19%和52.10%(P<0.05);4)C和D组乙酸比例显著高于A和B组(P<0.05);B组丙酸比例较A、C和D组高出4.24%、3.18%和6.43% (P<0.05),乙酸/丙酸显著低于A、C和D组(P<0.05);5)各处理组间血清总蛋白、葡萄糖和尿素氮含量无显著差异(P>0.05)。1~3月龄犊牛开食料中NDF适宜水平为15%有利于提高断奶后日增重,促进饲料利用效率,改善瘤胃内环境,且对犊牛血清生化指标无不利影响。     

盐胁迫对菘蓝幼苗生长和抗性生理的影响

以“定蓝1号”为试验材料,采用不同浓度NaCl溶液(0,30,60,90,120和150 mmol·L^-1)对菘蓝幼苗进行处理,胁迫14 d后测定了其生长指标、生物量积累、叶片相对含水量、根系活力、根质膜透性、渗透调节物质含量以及抗氧化酶活性等指标,分析不同程度盐胁迫对菘蓝幼苗生长和抗性生理的影响。结果表明:NaCl胁迫对菘蓝幼苗株高和根长都有抑制作用,对根和叶的物质积累也有显著影响,并随浓度的递增呈降低的趋势;菘蓝幼苗叶片相对含水量和根系活力均随NaCl浓度的增加呈现下降趋势,而根质膜透性、叶片脯氨酸含量则呈升高趋势;丙二醛(MDA)含量、组织自动氧化速率(RAD)、过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性都随NaCl浓度的增加呈先升高后降低的趋势。但当NaCl浓度低于90 mmol·L^-1时,菘蓝幼苗的生长没有受到盐胁迫的明显抑制,根系活力降低不明显,脯氨酸、丙二醛含量和保护酶活性也未显著升高,说明菘蓝幼苗对低浓度盐环境具有一定的耐受性。     

The influence of rapid growth on sodium salicylate pharmacokinetics in male turkeys

The aim of this study was to assess the influence of growth on the pharmacokinetics of sodium salicylate (SS) in male turkeys. SS was administered intravenously at a dose of 50 mg/kg. Plasma drug concentrations were assessed by high‐performance liquid chromatography, and pharmacokinetic parameters were calculated by noncompartmental analysis. As the age increased from 6 to 13 weeks (body weight increase from 2.35 to 9.43 kg), median body clearance decreased from 1.34 to 0.87 ml/min/kg. This caused a significant increase in the median mean residence time from 3.42 to 4.44 hr. Elimination phase proved to be biphasic and two elimination half‐lives (T_1/2el) were distinguished. Whereas T_1/2el1 was found to increase with age by 128%, T_1/2el2 represented a later but faster and less age‐dependent phase of elimination (increase by 56% in the respective groups). Volume of distribution decreased with age. These effects may lead to different therapeutic response to SS in turkeys of different age and body weights.     

Effect on Rendement Napole genotype on metabolic markers in Ossabaw pigs fed different levels of fat

We investigated effects of Rendement Napole (RN ) genotype on metabolic markers in Ossabaw pigs fed diets with different levels of dietary fat. Thirty‐two pigs, belonging to either the wild‐type (WT , rn⁺/rn⁺) or carrier (CAR , RN ^?/rn⁺) genotypes (n  = 16/genotype), were divided into two dietary groups, (high fat [HF ] or low fat [LF ]) diets, for 12 weeks (n  = 8 pigs/genotype/diet) after which pigs were killed for gene expression analysis by RT ‐PCR . Feeding HF diet caused increased daily gain (ADG , p  <  .05) and final body weight (BW ) (p  <  .05) in comparison with the LF diet (p  <  .05). Feed efficiency (gain:feed) was higher (p  <  .05) in pigs on the HF and was higher (p  <  .05) in CAR pigs compared to WT . There was genotype × diet interaction (p  =  .05) on final BW such that CAR animals on LF diet had the same final BW as animals of both genotypes on HF diet. Carrier pigs on LF diet had higher (p  <  .05) average daily gain and gain:feed than WT pigs. There was a trend (p  <  .08) for a higher feed consumption in pigs on the LF diet. Backfat thickness was higher (p  <  .01) in pigs on the HF diet. Serum triglyceride was higher (0.62 vs. 0.33 mg/dl, p  <  .01) in pigs on HF diet. Serum insulin was higher (p  <  .05) in CAR versus WT pigs (0.40 vs. 0.015 μg/ml). Pigs on the HF diet had a higher (p  <  .05) serum insulin compared to those on the LF diet (0.032 vs. 0.023 μg/ml). Carnitine palmitoyl transferase 1‐alpha was higher (p  <  .05) in the longissimus dorsi and semitendinosus muscles of pigs on HF diet. Acyl‐CoA oxidase I was elevated (p  <  .05) in the liver of pigs on HF diet. Fatty acid synthase was lower in the longissimus dorsi muscle, liver and mesenteric fat (p  <  .05) of carrier pigs. The RN gene regulates specific metabolic markers in the Ossabaw pigs.