用PCR检测副溶血性弧菌耐热溶血素基因

根据副溶血性弧菌的耐热溶血素基因（ｔｄｈ）设计了２对引物，经聚合酶链式反应（ＰＣＲ）检测，所有１８株神奈川试验阳性副溶血性弧菌均呈阳性反应，而６株神奈川试验阴性副溶血性弧菌呈阴性反应，其他９种（３４株）弧菌和８株其他革兰氏阴性菌也呈阴性反应。检测敏感性可达２ＣＦＵ的副溶血性弧菌。     

黄土丘陵区不同植被类型根际微生物群落功能多样性研究

为了解生态恢复过程中土壤微生物功能特征,采用Biolog-Eco微平板法分析了黄土丘陵区人工灌木(柠条 Caragana korshinskii、沙棘 Hippophaer rhamnoides)、人工草地(沙打旺 Astragalus adsurgens、柳枝稷 Panicum virgatum)和天然草地(阿尔泰狗哇花 Heteropappus altaicus、茵陈蒿 Artemisia capillaries)的根际微生物碳源代谢多样性特征。结果表明:柠条、阿尔泰狗哇花和茵陈蒿的根际微生物对糖类的代谢能力显著高于沙棘、沙打旺和柳枝稷;阿尔泰狗哇花和茵陈蒿对氨基酸类、酚酸类和聚合物类的代谢能力较高。茵陈蒿和阿尔泰狗哇花的微生物代谢功能多样性高于其他4种植物。柠条、阿尔泰狗哇花和茵陈蒿具有相似的根际微生物代谢功能。黄土丘陵区植被类型显著影响微生物群落碳源利用能力,天然草地根际微生物群落代谢功能多样性优于人工灌木和人工草地。     

丸粒化处理对胡麻种子萌发和幼苗生长的影响研究

采用二因素裂区设计,以保水剂和活性炭、硅藻土、凹凸棒为填充剂的包衣材料制作丸粒化胡麻种子,研究了丸粒化处理对胡麻种子萌发和幼苗生长的影响。结果表明:与不加保水剂相比,保水剂质量与种子质量为1∶1时,丸粒种子的活力指数和出苗率显著提高20.11%、18.62%;随着保水剂含量的增加,幼苗的根冠比显著减小3.25%。包衣剂中以活性炭:凹凸棒=1∶1为填充剂的发芽率和出苗率比不加填充剂的明显提高6.79%、22.41%,幼苗生长最健壮。与未丸粒化的种子相比,当保水剂质量与种子质量为1∶1时,以活性炭:凹凸棒=1∶1为填充剂的丸粒种子发芽指数、活力指数和出苗率显著提高,而且幼苗的茎粗、叶面积和根冠比分别显著增加11.02%、27.35%、28.07%。可见,适量的保水剂(保水剂质量与种子质量为1∶1)和以活性炭、凹凸棒为填充剂的包衣剂能提高胡麻种子活力,增加出苗率,使幼苗的干物质重增大,根系发达,根冠比提高,为胡麻高产奠定了良好的基础。     

Effects of ammonium and inorganic carbon enrichment on growth and yield of a hydroponic tomato crop

Effects of varying the proportions of NO₃^— and NH₄⁺ in the growth medium on seedling growth and tomato fruit yield (Lycopersicon esculentum L. cv. Trust F1) were investigated in greenhouse hydroponic experiments. The presence of NH₄⁺ as the sole N source (11 mM) was toxic: it curtailed growth and decreased chlorophyll content of the leaves. However, at low concentration (10 % of total N), the presence of NH₄⁺, with or without added dissolved inorganic carbon (DIC), increased vegetative growth and fruit yield by ˜ 15 %, and enhanced taste/flavor of the fruits. In DIC‐enriched treatment, pH was maintained at 5.8 by addition of KHCO₃ or as CaCO₃. The presence of NH₄⁺, at 10 % of total N, inhibited NO₃^— uptake rates by ˜ 27 %. The rates of uptake of NO₃^— and NH₄⁺ were comparable (13.3 and 14.2 mmol plant^—1 d^—1, respectively, in the presence of DIC, and 14.7 and 14.0 mmol plant^—1 d^—1, respectively, in the absence of DIC), despite such a large difference in their concentrations in the nutrient feed solution. A higher proportion of NH₄⁺ (up to 50 % of total N) had no further significant effect upon early vegetative growth, but in a long‐term experiment resulted in a high incidence of blossom end‐rot (BER) disease, thereby severely curtailing fruit yield. The presence of even 1.1 mM NH₄⁺ reduced Ca²⁺ and Mg²⁺ accumulation in the leaves as well as in fruits.     

Chemical changes of kenaf core binderless boards during hot pressing (I): influence of the pressing temperature condition

Self-bonding is the main factor of the performance expression of binderless boards, and therefore its clarification is considered to be an important issue. For this purpose, a series of chemical analyses were conducted on kenaf core binderless boards and their chemical changes during the hot-pressing process are discussed in this article. First of all, binderless boards were prepared from kenaf core powder at different pressing temperatures (without steam-explosion process) and were used for chemical analyses after they were reduced into powders and extracted with methanol. To investigate their chemical changes, lignin, holocellulose, and neutral sugar contents were determined, Fourier transform infrared (FTIR) spectra were recorded, and the nitrobenzene oxidation procedure was applied. As a result, it was found that parts of lignin and hemicelullose were decomposed during the hot-pressing process; however, the contribution of the resulting fractions to selfbonding was not observed. In addition, progress of condensation reactions in lignin and the formation of chemical bonds by low molecular weight conjugated carbonyl compounds in methanol extractives were observed. Thermal softening of lignin is also suggested to play an important role in the expression of board performance.     

八个竹种海涂引种试验报告

本文从土壤含盐量着手对八个竹种在引种成活率、新竹抽发数及其质量、角竹笋产量等方面进行了较系统的研究。结果表明,土壤盐分影响竹子的生长发育,此影响一般随含盐量的增加而增加;不同竹种对土壤盐分的适应能力不同;角竹笋能获得高产,单产达20800kg/ha。参照产地母竹和引种地新竹的生长情况,我们建议:海涂地区发展竹子时可选用角竹、桂竹和红竹。     

基于键合图理论的动力稳定装置轮轨间动态特性分析

考虑动力稳定装置的轮轨非线性接触、轮轨间的间隙传动、夹钳油缸和水平油缸的等效刚度建立了动力稳定装置动态特性键合图模型.使用分段函数表达式实现了轮轨间的间隙传动.针对不同夹持力对于能量的传递效率与作业效果进行了深入分析与探究,确定了所需最小的水平油缸的推力.     

Statistical Model on the Time and Time Difference Error of Arrival in TDOA Location Estimation

Additional time delay of arrival resulted from non-line of sight(NLOS)propagation and muti-path effect in(cellular) environment is the dominant factor resulting in TDOA location error,study on the statistical property of TDOA error is useful for improving the accuracy of TDOA location.Based on the exponential distribution property of NLOS propagation delay,and considering the system error resulted from the signal detection,the authors establish a statistical model of arrival time and a model of TDOA error distribution by statistics when non-light of sight and multi-path propagation exist.The models reflect the statistical property of arrival time and TDOA error in cell network,the simulation shows that the proposed models have a good accuracy and a satisfactory behavior.     

丽水市责任山产权登记现状分析与探讨

通过对浙江省丽水市责任山产权登记现状的调查,分析各县、市、区存在登记差异的原因,并提出了解决问题的建议。