Sensitivity of populations ofBotrytis cinerea to triazoles,benomyl and vinclozolin

Sensitivity of field isolates (121) ofBotrytis cinerea from France (1992), Germany (1979–1992), Israel (1990) and the Netherlands (1970–1989) to the triazoles tebuconazole and triadimenol, the benzimidazole benomyl and the dicarboximide vinclozolin were tested in radial growth experiments. Resistance to benomyl (in 21 to 100% of isolates tested) and vinclozolin (in 25 to 71% of isolates tested) was common in most countries. EC₅₀s (concentrations of fungicides inhibiting radial mycelial growth ofB. cinerea on B5-agar by 50%) for tebuconazole and triadimenol ranged between 0.01–1.64 and 0.4–32.6g ml^–1, respectively, and were log-normally distributed. The variation factor (ratio between EC₅₀s of the least and most sensitive isolate tested) amounts 164 and 82 for tebuconazole and triadimenol, respectively. These values are comparable to those for azole fungicides applied in control of other pathogens. Hence, variation in sensitivity to triazoles can probably not explain limited field performance of triazoles towardsB. cinerea. Isolates from south west Germany (1992) were significantly less sensitive to tebuconazole than isolates collected earlier in Germany, Israel and the Netherlands. Such less sensitive populations may contribute to the limited field performance of DMI fungicides towardsB. cinerea. The sensitivity of isolates from south west Germany to tebuconazole was similar to that of DMI-resistant mutants generated in the laboratory. These mutants displayed stable resistance with Q-values (ratio between EC₅₀ of resistant mutant and wild type isolate) between 5 and 20. Sensitivity of field isolates and laboratory mutants to tebuconazole and triadimenol was correlated.     

Genotypic diversity and migration of clonal lineages of Botrytis cinerea from chickpea fields of Bangladesh inferred by microsatellite markers

An analysis of allelic diversity at nine microsatellite loci provided an insight into the population structure of Botrytis cinerea from four fields (sampled in 2003 and 2004) that represented important regional locations for chickpea production in Bangladesh. Although three populations were limited by sample size after clone‐correction, a total of 51 alleles were amplified among 146 B. cinerea isolates from Bangladesh, which revealed a high amount of within‐population and overall genetic diversity (H_S = 0·48 and H_T = 0·54, respectively). The percentage of maximal genotypic diversity (G) ranged between populations (G = 23–40), with a total of 69 haplotypes detected (G = 25). Bayesian cluster analysis depicted two major clusters distributed among the four Bangladesh populations, indicating population admixture from two origins that have spread throughout these regions. Genotype flow between regions was detected and indicated the spread of clonal lineages, consistent with relatively low differentiation among the four populations (mean G_ST = 0·1, P < 0·05). These results highlighted the potential threat of host resistance breakdown as a result of considerable genetic diversity, genotype flow and the evolutionary potential of B. cinerea.     

内蒙古天然草地退化成因的多因素灰色关联分析

选取内蒙古草地典型草原、草甸草原及农牧交错带典型区,通过对区域内影响草地退化的社会、经济和环境等因素进行定性分析,应用灰色系统理论的关联分析方法,以草地退化面积为参考数列,以牧草生长季积温、降水变率和年大风日数等气候因子,草地超载比例、人工种草与围栏草场面积、年割草量、畜棚与畜圈面积和牧民人均收入等人类活动因子作为比较数列,对草地退化进行了多因素关联分析。研究结果客观、定量地揭示了草地退化与各因素间的相互关系,认为草地生态系统的退化受损是气候变化和人类活动共同胁迫的结果,人类对草地的管理方式对草地生态系统具有巨大影响,人类可以通过多种管理措施来减轻牲畜对草场的压力,积极解决人与资源的平衡。     

Nutritional Enhancement of Biocontrol Activity of Candida sake (CPA-1) against Penicillium expansum on Apples and Pears

Pome fruits are poor in nitrogenous compounds and the addition of nitrogen can improve colonisation of the fruits by antagonists. Twenty-two nitrogenous compounds were evaluated for their effect on Candida sake (CPA-1) growth in vitro. Ten compounds that induced greater growth were applied with the antagonist to wounded fruits to evaluate their effect on enhancing control of Penicillium expansum. Calcium chloride and 2-deoxy-D-glucose were also tested. L-serine and L-aspartic acid enhanced biocontrol by C. sake against P. expansum on apples. On apples and pears, ammonium molybdate, calcium chloride and 2-deoxy-D-glucose improved the capacity of the antagonist to control P. expansum. The addition of ammonium molybdate at 1 mM allowed C. sake to be used on apples and pears at a lower concentration without diminishing control. Similar results were observed with the addition of calcium chloride to the antagonist. 2-deoxy-D-glucose at 6 and 18 mM enhanced biocontrol on pears by over 81%, but on apples the improvement of biocontrol was observed only at 6 mM. In cold storage, the combination of ammonium molybdate and C. sake completely eliminated the incidence of blue mould on pears, and reduced its severity and incidence by more than 80% on apples.     

Structure and Expression In planta of Botrytis cinerea Ubiquitin Genes

To identify genes of the necrotrophic pathogenic fungus Botrytis cinerea that are expressed during infection of tomato leaves, a differential screening of a genomic library with radioactively labelled cDNA was performed. This resulted in the identification of a B. cinerea gene, denominated Bcubi4, which encodes a precursor protein consisting of four identical head-to-tail repeats of a 76aa ubiquitin unit. Subsequently a gene denominated Bcubi1CEP79, encoding a single ubiquitin unit joined to a Carboxyl Extension Protein of 79 amino acids, was isolated. The expression of the two ubiquitin genes was studied during pathogenesis of B. cinerea on tomato. Bcubi1CEP79, but not Bcubi4, mRNA was transiently induced at 16h after inoculation. The increased expression of the Bcubi1CEP79 gene at this stage of pathogenesis might be required for enhanced ribosomal biogenesis.     

灰色关联度分析法对不同处理下草坪质量的综合评判

应用灰色系统理论对不同施氮量和施肥次数处理下的草坪质量进行了分析与评判.结果表明:施用氮肥是提高草坪质量的重要措施,在35 g/m2施量水平下,草坪质量与施肥量呈正相关(R2＞0.9100);施肥次数对其影响不显著.建议在实践中施肥量以25 g/m2为宜.     

Chitosan Stimulates Defense Reactions in Grapevine Leaves and Inhibits Development of Botrytis Cinerea

Chitosan (β-1,4-linked glucosamine oligomer) derived from crab shells conferred a high protection of grapevine leaves against grey mould caused by Botrytis cinerea. Under controlled conditions, it was shown to be an efficient elicitor of some defense reactions in grapevine leaves and to inhibit directly the in vitro development of B. cinerea. Treatment of grapevine leaves by chitosan led to marked induction of lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL) and chitinase activities, three markers of plant defense responses. Dose-response curves show that maximum defense reactions (PAL and chitinase activities) and strong reduction of B. cinerea infection were achieved with 75–150 mg l⁻¹ chitosan. However, greater concentrations of chitosan did not protect grapevine leaves with the same efficiency, but inhibited mycelial growth in vitro. Present results underlined the potency of chitosan in inducing some defense responses in grapevine leaves which in turn might improve resistance to grey mould.     

乌鲁木齐河流域土地灌溉系统的灰色决策模型研究

本文以乌鲁木齐河流域为试验区,介绍了干旱区流域土地灌溉系统的多目标灰色决策模型的构造原理和方法。利用灰色控制系统的理论和方法建立了灰色优势分析模型,并以该模型为基础建造了多目标灰色决策分析模型。对最优局势的效果测度与综合决策矩阵的构成原理进行了深入探讨,并由此构造了地下水位控制模型与土地灌溉系统的灰色决策模型,为流域地下水位的控制提供了一种新的研究方法。     

放牧环境条件下育成公羊营养物质消化代谢的研究

本研究是在内蒙古白音锡勒种畜场自然放牧条件下进行的，按季节及自然气温分为四期，进行物质消化代谢及气体能量代谢试验，同时监测环境温度、湿度、风速及日照时数等气象指标。采用灰色关联度法分析探讨在放牧条件下，牧草营养因素和外界环境因素对育成公羊能量及营养物质代谢的影响。     

应用灰色系统理论评价草地质量初探

以草地优良牧草产量、可食牧草产量、载畜量、牧草利用率和覆盖度为主要指标，应用灰色系统理论中关联工分析的方法，对武威地区３２个类型草地质量进行了评价。结果表明，质量最好的草地是山地草甸，质量最关的是干荒漠和荒漠摹。根据分析结果，提出了武威地区草地质量分级的方案。