道路景观设计方法探讨

提出对道路景观的规划、调查、定位等系统分析,从而在道路的形态、周边环境的借景及路域生态系统的景观绿化等方面,探讨了道路景观设计的方法。     

蒙古栎秋播种育苗技术的研究

自１９９３年起对本局大趟子苗圃进行了蒙古栎秋播育苗试验,并对蒙古栎从采种、种子调制、田间管理及起苗、留床、造林等环节上形成了一套完整的科学育苗方法,避免了春播育苗种子储藏和鼠害等一系列不利问题,研究探索了最佳播种量和最佳经营密度。     

引进温室带给中国设施园艺现代化的思考

中国引进现代化温室面积已逾２００ｈｍ＾２,对中国温室业的发展起到了积极的推动和示范作用。本在回顾和总结引进温室的基础上,通过实例分析对中国未来现代化温室的发展和建设提出了一些借鉴经验,尤其在温室节能、温室管理等方面就在地制宜发展中国设施园艺工程提出了一些具体的看法。     

桃(Prunus persica L.)茎尖培养技术的研究

筛选出了适合于桃品种‘北农早艳’茎尖(0.2 mm 和0.5 mm)培养的培养基为:G培养基附加 IBA 0.25 mg/L+BA 0.5 mg/L+GA_3 1.0 mg/L+LH 500.0 mg/L。茎尖用聚氨酯海绵作支撑物的液体培养比琼脂培养成活率高2～5倍,且生长迅速。获得了0.2 mm 长茎尖的生根植株。     

美国车厘子组织培养试验

采用美国思加图车厘子室内盆栽萌发的嫩芽和老芽作为外植体,分析不同处理时间对车厘子外植体萌芽效果的影响;采用不同浓度的植物生长调节剂对其进行诱导增殖和生根培养,筛选最佳组培方法。结果表明：嫩芽是车厘子外植体的最佳选择,通过7 s酒精和0.1%升汞9 min消毒获得无菌体,污染率低、褐化率较低、萌芽率60%。芽诱导培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,增殖系数为3.92。生根培养基为1/2MS+NAA0.6 mg/L,生根率为100%。移栽炼苗基质比例为V（腐殖土）∶V（红土）=4∶6,盖膜驯化,保湿温度（25±2）℃,湿度80%,成活率达90%以上。     

crataegus grayana的组织培养

同许多种其它果树一样,山植属的Crataegus grayana也可应用组织培养的方法,进行快速繁殖。在加入0.25ppmBA,0.5ppmIAA以及150ppm水解干酪素的MS培养基上,可由胚得到实生苗。在加入0.5ppm和300ppm水解干酪素的MS培养基上,由茎尖与茎段可得到壮实的试管苗。由于该植物胚在第一年内成熟度甚低,因此用茎尖或茎段进行快繁殖远优于用胚来进行。从此项研究结果看,其繁殖率为2~n-3~n(n为∮月数)。     

草莓快速繁殖技术的研究

本文探讨了利用茎尖离体培养法进行草莓快速繁殖的方法。主要摸索了激素对试管苗的分化、生根的影响及移栽成活的技术要点。     

Detection of an Ehrlichia bovis-like Organism in Cultured Buffalo Monocytes

Monocytes from a buffalo were cultured in RPMI 1640 medium following separation of plasma by the erythrocyte sedimentation technique and subsequent separation of mononuclear cells by density gradient centrifugation. Growth of an organism considered to be Ehrlichia bovis was noticed in the cultured monocytes after 10 days. The inclusions were considered to be those of E. bovis from their morphology, staining characteristics and growth characteristics in culture, and by indirect immunofluorescence examination with an anti-E. canis serum. The utility of peripheral blood monocyte cultures opens the possibility of diagnosing the carrier status of ehrlichiosis in animals.     

新生广西巴马小型猪肾成纤维细胞体外培养体系的建立

利用微量液体组织块贴壁法原代培养分离新生广西巴马小型猪肾成纤维细胞,进行常规传代培养、冷冻和复苏;通过接触抑制和解除抑制研究该类细胞的增殖潜力,免疫荧光进行鉴定;并通过脂质体介导对分离到的新生广西巴马小型猪肾成纤维细胞分别进行EGFP-N1和DsRed-N1基因转染。结果成功分离到新生广西巴马小型猪肾成纤维细胞,体外可以大量传代和冷冻,目前已经传至50代以上,生长良好;冷冻复苏和接触抑制解除后仍可以正常培养和传代;细胞免疫荧光检测,波形蛋白表达阳性,角形蛋白表达阴性;转染48h后荧光显微镜下可以观察到绿色荧光和红色荧光,表明瞬时转染新生广西巴马小型猪肾成纤维细胞可以获得成功,其中转染DsRed-N1基因的新生广西巴马小型猪肾成纤维细胞已经传至60代以上。结果表明,本试验成功建立了新生广西巴马小型猪肾成纤维细胞的体外培养体系,在体外能稳定培养和传代,并可以成功获得转基因的新生广西巴马小型猪肾成纤维细胞。     

Murine xenograft model demonstrates significant radio-sensitising effect of liposomal doxorubicin in a combination therapy for Feline Injection Site Sarcoma

Therapy of cats suffering from feline injection site sarcomas (FISS) is still a challenging problem, as the recurrence rate after surgery is up to 70%. Four FISS-derived primary tumour cell lines and corresponding xenograft tumour mouse models were established to evaluate the efficacy of a concomitant chemo-/radiation therapy with doxorubicin. In vitro, strongly depending upon the timing of administration, pre-treatment with 0.25 µmol doxorubicin resulted in a significant enhancement of radiation-induced (3.5 Gy) tumour cell death. This result was confirmed in vivo, where only the combined chemo-/radiation therapy resulted in a significant reduction in tumour growth compared to the respective mono-therapies with either doxorubicin or radiation. These results support the use of the concomitant chemo-/radiation therapy for adjuvant treatment of FISS, particularly in advanced or recurrent disease where surgery alone is no longer feasible.