Sentinel lymph node mapping by near‐infrared fluorescence imaging and contrast‐enhanced ultrasound in healthy dogs

Sentinel lymph node (SLN) mapping is a valuable and crucial diagnostic procedure in staging malignancies. We compared two non‐invasive techniques, near‐infrared (NIR) fluorescence imaging and contrast‐enhanced ultrasound (CEUS), to identify the SLNs in three superficial anatomical regions in an animal model. Six healthy laboratory dogs were included in a proof‐of‐concept trial. A NIR fluorescent dye (Indocyanine Green) and microbubbles (Sonovue) were consecutively injected subdermally in the Inguinal, axillary and popliteal region to map the SLNs. Transcutaneous NIR fluorescence imaging identified SLNs in 17 out of a total of 18 occasions. CEUS identified SLNs in all regions (18/18). Whereas NIR fluorescence imaging performed better in the visualization of the afferent lymphatic tract, CEUS demonstrated different filling patterns of the SLNs, a feature potentially critical for the concept of SLN mapping in cancer patients. Both NIR fluorescence imaging and CEUS are safe, non‐invasive, practical and accurate methods to perform real‐time transcutaneous SLN mapping with potential in a clinical setting.     

干旱荒漠区4种一年生植物种子萌发期耐盐性

采用不同浓度的NaCl溶液对4种一年生草本植物雾冰藜(Bassia dasyphylla)、白茎盐生草(Halogeton arachnoideus)、沙米(Agriophyllum squarrosum)和画眉草(Eragrostis pilosa)种子进行处理,通过测定种子相对发芽率、相对发芽势、发芽指数等指标,分析了种子萌发期耐盐差异性。结果表明:1)随着NaCl溶液浓度的增大,种子发芽率呈逐渐下降的趋势,在蒸馏水中第3天达到了发芽高峰,发芽基本结束,在NaCl溶液中第7天结束发芽。2)随NaCl溶液浓度逐渐增大,4种植物种子发芽率和发芽势均呈先增加后降低的趋势,同一植物种子发芽率和发芽势在不同浓度NaCl溶液中存在显著差异(P <0.05)。NaCl溶液浓度对白茎盐生草种子发芽率和发芽势影响较大,画眉草次之,其余两种较小。3)随NaCl溶液浓度逐渐增大,4种植物种子发芽指数呈下降趋势,雾冰藜活力指数显著高于白茎盐生草、沙米和画眉草种子;盐胁迫对画眉草发芽指数和活力指数影响较大,当NaCl溶液浓度达到2.1%时,幼苗活力指数降到最低。4) 4种植物幼苗长度均随NaCl浓度增...     

不同日光暖棚羊舍的内环境指标比较

在青海省乐都县养殖基地选择了有代表性的两种日光暖棚羊舍，对其主要内环境指标进行了对比。结果表明，半拱式塑料覆膜暖棚羊舍内的温度、湿度、CO2和NH3浓度均高于双列式玻璃钢瓦暖棚羊舍。两种类型日光暖棚羊舍各有利弊。     

排卵延迟奶牛发情后不同阶段B超黄体相关指标变化规律分析

为研究排卵延迟奶牛发情配种后黄体的变化规律及其差异性,本研究在临床症状观察、直肠检查和B超辅助检查诊断的基础上,选取8头排卵延迟奶牛(排卵延迟组)、5头健康正常发情奶牛(对照组),应用B超对黄体的相关指标(直径、面积、周长和体积)进行了测量及分析,对获得的不同阶段典型黄体声像图进行了描述。结果显示,与对照组相比,排卵延迟组与对照组奶牛黄体的直径、面积、周长和体积在发情配种后7~15 d差异均不显著(P>0.05)。在发情配种后第9和13天排卵延迟组奶牛黄体相关指标(直径、体积、面积和周长)大于对照组奶牛,而在第11天小于对照组奶牛。结果表明,排卵延迟奶牛发情后不同阶段B超黄体直径、面积、周长和体积与正常发情排卵后的相应指标没有显著差别。     

日粮添加酵母硒对蛋鸡血清生化指标的影响

本试验在蛋鸡日粮中添加不同浓度的酵母硒,测定蛋鸡血清生化指标,旨在探究酵母硒对蛋鸡肝脏功能的影响。结果表明:日粮添加酵母硒对蛋鸡血清谷草转氨酶(Aspartate aminotransferase,AST)、谷丙转氨酶(Alanine aminotransferase,ALT)、总胆红素(Total bilirubin,TBIL)无显著影响;日粮添加酵母硒显著降低了血清总胆固醇(Total cholesterol,TC)含量,其中0.80 mg/kg的酵母硒效果最好,第28天比对照组下降了38.06%。认为日粮添加酵母硒没有对蛋鸡肝脏功能产生不良影响,且有保护心血管的作用。     

细胞工程技术选育的草地早熟禾新种质的耐热性分析

通过对离体培养的草地早熟禾丛生芽块进行高温处理,筛选出一批耐热性好的小芽。小芽成苗后移栽到花盆在自然条件下生长。半年后对这些株系和原品种的植株进行热胁迫处理,同时测定叶绿素荧光、丙二醛含量、离子渗漏率、叶绿素含量等生理指标的变化,得出细胞工程株系的植株耐热性明显较高,热胁迫处理后损伤程度小于原品种植株。表明利用细胞工程技术能有效获得耐热性显著提高的草地早熟禾植株,为选育草地早熟禾耐热新种质提供材料。     

饲料加工企业内控评价指标体系的构建及其应用研究

面对日益复杂的竞争形势,饲料加工企业需要加强内控管理.为了评价内控体系的有效性,通常寻找一些评价指标形成体系对内控进行评价.本文提出构建内控评价指标体系需要一些前提,包括对关键环节的识别、评价原则的确定等,在指标选取中,至少应涵盖公司层面、业务层面、财务层面和合法合规层面的指标.内控评价体系构建后,需要有组织保障和制度...     

家兔乳酸脱氢酶等生化指标的测定及其与生产力关系的研究

对８个品种家兔的血清Ｃａ、Ｐ、Ｋ、Ｎａ、乳酸脱氢酶和血清蛋白质含量的测定并经ＬＳＲ检验表明，各品种家兔之间乳酸脱氢酶及其同工酶含量不同，其生产性能与Ａ、Ｂ亚基含量相关，含Ａ亚基越丰富，其生长速度越快；各品种间血清总蛋白、白蛋白、球蛋白含量、白／球比值及β－球蛋白含量均存在不同差异，生长发育快的家兔的血清总蛋白、球蛋白、β－球蛋白含量较高，白蛋白含量及白／球比值较低     

Length of lags in responses of milk yield and somatic cell score on test day to heat stress in Holsteins

We used daily records from provincial Japanese weather stations and monthly test‐day records of milk production to investigate the length of the lags in the responses of cows’ milk yield and somatic cell score (SCS) to heat stress (HS). We also investigated the HS thresholds in milk yield and SCS. Data were a total of 17,245,709 test‐day records for milk and SCS in Holstein cows that had calved for the first time between 2000 and 2015, along with weather records from 60 weather stations. Temperature–humidity index (THI) values were estimated by using average daily temperature and average daily relative humidity. Adjusted THI values were calculated by using temperature, relative humidity, wind speed, and solar radiation. The model contained herd, calving year, month of test day, age group, days in milk, and THI as a fixed effect. THIs for each day from 14 days before the test day until the test day were used to represent the HS effects. The HS occurring 3 days, and between 8 and 10 days, before the test day had the greatest effect on the milk yield and SCS, respectively. The threshold THI values for the HS effect were about 60–65 for both traits.