果园肥料深施机成孔机构设计与试验

针对密植林果园机具通过性差,以及表层施肥的肥料利用率较低的难题,提出了将颗粒肥定量挖坑深施的施肥方法,围绕该方法,设计了一种小型果园肥料深施机,并对整机进行了挖坑性能验证试验。结果表明：挖坑深度在300、350、400 mm时的坑穴上口平均直径为207.7、207.1、209.9 mm,坑穴下口平均直径为206.3、204.3、206.1 mm;挖坑深度平均值为296.7、345.9、395.4 mm,坑穴直径误差5 mm以内,深度误差在5 mm以内;挖坑效率分别为34.5、39.6、44.1 s,能够满足挖坑要求;试验数据给果园肥料深施机的产业化提供了技术参考。     

镉胁迫对冬小麦根端细胞超微结构的影响

用透射电镜观察镉处理对冬小麦幼苗根端细胞超微结构的影响，结果表明，ＣｄＣｌ２２．５Ｈ２Ｏ浓度较低时（１０×１０＾－６），可见被观察的细胞表现为核膜凹陷，胞核变形，染色质稍显凝聚；同样浓度下线粒体的超微结构未见明显变化。ＣｄＣｌ２ ２．５Ｈ２Ｏ浓度较高时（８０×１０＾－６），则见细胞核膜破裂。崩解，染色质显著凝聚；线粒体内膜解体，嵴模糊或消失，表现为不可逆转性变化。     

羊乳腺上皮细胞的形态观察

用组织块培养法获得山羊乳腺细胞原代培养物,并根据山羊乳腺成纤维细胞与上皮细胞对胰蛋白酶的敏感性不同将二者分离纯化。对细胞形态进行光镜观察发现：纯化的山羊乳腺上皮细胞传代至第15代时其生长仍正常。山羊乳腺上皮细胞含不同的细胞类型,大多数上皮细胞呈短梭形或多角形,呈蜂窝状;部分细胞呈圆饼状,体积较大;部分细胞呈长形。纯化的乳腺上皮细胞增殖可形成圆顶型结构,呈乳头状,称之为乳球体。     

Mechanism of Influence of the Explosive Blast on the Permeability of the Pulmonary Microvascular Endothelial Cells Monolayer

The influence of the explosive blast with different pressure on the permeability of the pulmonary microvascular endothelial cells monolayer (PMECM) has been observed by using the model of the explosive blast wounded the endothelial cells monolayer in vitro The mechanism of the effect of the blast on PMECM permeability has also been explored in pathology and cytoskeleton. The results show that the explosive blast with about 100 kPa pressure can increase the permeability of PMECM in conductance detection, and the explosive blast with lower than 350 kPa pressure can mainly cause the increasing of the gap between endothelial cells, and explosive blast with higher than 600 kPa pressure can exfoliate the endothelial cells monolayer. The occurrence mechanism of PMECM permeability changes caused by explosive blast is related to the change of the cytoskeleton myosin.     

Relationship of Integrins and Hepatocellular Carcinoma(HCC) Cells during Invasion and Metastasis

This is the first key step of carcinoma cells attachment to the extracellular matrix (ECM), which is mediated by the special receptors of cell's exterior. Integrins belong to the most important attachment molecular. A brief review on hepatocellular carcinoma (HCC) cells' integrins & ECM pertinent during invasion and metastasis is given. The recognition of integrins mediating HCC cell-cell adhesion & HCC cells attachment to ECM and integrins' expression during chemotaxis helps us to understand the significant role of integrins during the whole process. The integrins relate to the process of HCC' invasion and metastasis. People are looking forward to anti-integrins so as to interdict or weaken the reciprocity between integrins & ligands, which must bring aspiring effect to the research on anti-cancer medicine.     

Development of High-speed Rectangle Burner Used in Baked Aluminum Reduction Cells

Bake out of Aluminum reduction cells will affect the normal production and the ultimate life of reduction cell directly. The method of thermal bake out is a key of novel technology which possesses such advantages as well distributed temperature, easy control for the speed of heating up, fully baking for the joint and ramming paste of the edge. The design of the burner is a key technology of thermal bake out. A new high speed rectangle burner has been developed based on the experiment, and the structure of this burner has also been optimized. The new burner an fire easily, burn stably and fully, and has high ratio of load adjustment, and low noise, etc.. It can meet the needs of thermal bake out reduction cells. Its development can provide the technological guarantee for the use of thermal bake out Aluminum reduction cells.     

剑麻叶片纤维细胞形态结构差异分析

观察分析剑麻叶片纤维细胞形态结构特征,及明确取样部位,为深入研究剑麻纤维细胞发育机制及形态提供基础性和实验性的参考。根据剑麻叶片及植株植物学特征确定取样部位,利用透射电镜（TEM）观察2个生长时期成熟叶片的不同区域和3个成熟度叶近轴端纤维细胞形态结构的差异。由电镜图片可知,剑麻不同生长时期叶片的中部和近轴端纤维细胞紧密相连、大小不一,但叶片中部区域多为不含原生质体的细胞壁结构,而近轴端细胞中存在含有细胞器的原生质体。进一步观察了3个成熟度叶片近轴端的纤维细胞形态,发现叶片纤维细胞中的各种细胞器被液泡挤压到细胞边缘,叶绿体为最突出的细胞器。幼年叶和成熟叶中的叶绿体多呈梭形,外膜完整,可见较清晰的片层结构和垛叠区,稚嫩叶的细胞中叶绿体出现皱缩及片层结构排列较紊乱,线粒体肿胀,胞浆内出现空泡,存在细胞核中异染色质增多和质壁分离的现象。综合分析图像信息推测,剑麻叶片纤维细胞生长发育活跃部位位于叶片近轴端,是观察剑麻纤维细胞结构的理想区域。     

低糖颗粒型草莓酱的研制与工业化生产的探讨

以草莓为原料,采用二次真空渗糖与复合增稠剂调配相结合的方法,进行了低糖草莓酱的研制,并探讨了草莓酱工业化生产的问题。结果显示,低糖草莓酱的可溶性固形物含量大于25%,糖度为25%左右,pH值为3.0,产品色泽自然,滋味宜人。     

山羊乳腺上皮细胞的光镜观察

采用电穿孔转化法,将EGFP(增强型绿色荧光蛋白)基因转化体外培养的山羊乳腺上皮细胞。细胞穿孔转化7d后在荧光显微镜下观察。结果显示,EGFP基因成功地转入体外培养的山羊乳腺上皮细胞,EGFP基因获得表达,6种电穿孔参数的转化效果基本相同。     

好氧颗粒污泥处理城市污水的农田适灌性

利用好氧颗粒污泥处理城市污水,探讨城市污水用于农田灌溉的可行性.研究表明:经过好氧颗粒污泥处理过的污水,污水中的化学需氧量为130 mg/L,悬浮物为50 mg/L,总氮20 mg/L,总磷只有1.5 mg/L,出水中的COD_Cr和BOD₅去除率达到60%和55%;好氧颗粒污泥对污水中的氮、磷去除效果一般,去除率分别为50%和53%;出水中重金属去除率比较高,镉质量=浓度1.0×10^-5 mg/L,小于《农田灌溉水质标准》镉的标准值0.006 mg/L,出水中铅质量=浓度0.02 mg/L,小于标准值0.1 mg/L,出水中锌、铜、汞、铬的质量=浓度也远远低于标准中的质量=浓度值.结果表明,好氧颗粒污泥处理过的城市污水的出水可以用于农田灌溉,出水中COD_Cr和BOD₅、氮、磷、重金属等指标完全达到《农田灌溉水质标准》要求.