全文获取类型
收费全文 | 4192篇 |
免费 | 206篇 |
国内免费 | 463篇 |
专业分类
林业 | 81篇 |
农学 | 126篇 |
基础科学 | 55篇 |
182篇 | |
综合类 | 962篇 |
农作物 | 144篇 |
水产渔业 | 234篇 |
畜牧兽医 | 1717篇 |
园艺 | 1317篇 |
植物保护 | 43篇 |
出版年
2024年 | 13篇 |
2023年 | 43篇 |
2022年 | 163篇 |
2021年 | 178篇 |
2020年 | 178篇 |
2019年 | 245篇 |
2018年 | 144篇 |
2017年 | 202篇 |
2016年 | 234篇 |
2015年 | 232篇 |
2014年 | 250篇 |
2013年 | 295篇 |
2012年 | 343篇 |
2011年 | 343篇 |
2010年 | 179篇 |
2009年 | 177篇 |
2008年 | 207篇 |
2007年 | 315篇 |
2006年 | 168篇 |
2005年 | 119篇 |
2004年 | 157篇 |
2003年 | 113篇 |
2002年 | 88篇 |
2001年 | 74篇 |
2000年 | 74篇 |
1999年 | 37篇 |
1998年 | 31篇 |
1997年 | 22篇 |
1996年 | 27篇 |
1995年 | 23篇 |
1994年 | 30篇 |
1993年 | 36篇 |
1992年 | 19篇 |
1991年 | 9篇 |
1990年 | 15篇 |
1989年 | 15篇 |
1988年 | 16篇 |
1987年 | 10篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1973年 | 1篇 |
1956年 | 8篇 |
排序方式: 共有4861条查询结果,搜索用时 31 毫秒
101.
AIM To investigate the effects of 17β-estradiol (E2) treatment on the mesenteric lymphatic microcirculation and isolated lymphatic contractility in rats after hemorrhagic shock, and to explore the relationship between contractility and the difference between intra- and extracellular calcium ion concentrations ([Ca2+]) of lymphatic smooth muscle cells (LSMCs). METHODS Male Wistar rats were divided into sham group, shock group and shock+E2 group. The rats were subjected to hemorrhage [(40±2) mmHg for 90 min] and resuscitation with or without subcutaneous injection of E2 (2 mg/kg). After resuscitation for 3 h, the mesenteric lymphatic microcirculation in vivo was observed. Moreover, the isolated mesenteric microlymphatic rings were prepared for the observations of lymphatic contractility evaluated by the indexes including end-systolic diameter, end-diastolic diameter, contraction frequency (CF) and passive diameter. Meanwhile, the difference between intra- and extracellular [Ca2+] of LSMCs was recorded during lymphatic contraction. RESULTS Treatment with E2 significantly enhanced the CF, total contractile fraction and lymphatic dynamics index in vivo in the rats after hemorrhagic shock, and increased the CF, the fractional pump flow and the difference between intra- and extracellular [Ca2+] of LSMCs in isolated lymphatics from the shocked rats (P <0.05). CONCLUSION Estrogen treatment enhances lymphatic contractility in rats after hemorrhagic shock, which is related to enhancement of difference between intra- and extracellular [Ca2+] of LSMCs. 相似文献
102.
103.
Mast cells are important cells for the innate immunity that reside in tissues including adipose tissue and are involved in various physiological and pathological processes by producing a range of biological mediators. Adipose tissue not only acts as an energy depot and regulator of energy homeostasis that can deposit excess energy and dissipate energy through heat, but also is an active endocrine organ capable of producing hormones and adipokines. The dysfunction of adipose tissue is highly correlated with metabolic disorders, such as obesity, type 2 diabetes mellitus and so on. This review focuses on the physiological and pathological roles of mast cells in adipose tissue. 相似文献
104.
AIM To construct the mouse embryonic stem cell (ESC) line with stable pancreatic and duodenal homeobox 1 (Pdx1 ) expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1+ definitive endoderm cells into pancreatic cells. METHODS The Pdx1 -overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups: blank control group (ESC group), empty lentivirus control group (PDX1- ESC group) and Pdx1 lentivirus transfection group (PDX1+ ESC group). Flow cytometry was used to detect the transfected cells after screening by doxycycline (DOX). The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1- ESC group and PDX1+ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS (1) The positive rates of transfected cells in PDX1- ESC group and PDX1+ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1- ESC group and PDX1+ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. (2) With DOX, green fluorescence was observed in PDX1- ESC group and PDX1+ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1+ ESC group (P <0.05). Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed (P >0.05). (3) After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1+ definitive endoderm cells into pancreatic cells. 相似文献
105.
经电镜扫描寡齿新银鱼的第Ⅳ时相,第Ⅴ时相卵母细胞和受卵的卵细胞粘丝,结果表明,卵细胞表面具从受精孔发生的12根左右粘丝,此粘丝于受精孔处微弱弯曲,经侧部渐向受精孔相对处呈现很强扭曲或互相缠绕。卵细胞粘丝不仅出现在受精卵和第Ⅴ时相卵母细胞表面,而且清晰地出现在第Ⅳ时相卵母细胞的滤泡膜上。 相似文献
106.
大银鱼卵细胞粘丝扫描电镜观察 总被引:1,自引:0,他引:1
用扫描电镜观察了大银鱼的第Ⅲ时相,第Ⅳ时相,第Ⅴ时相卵母细胞和受精卵的卵细胞粘丝的外部形态特征和它们的粘性,并讨论了卵细胞粘丝的功能。 相似文献
107.
108.
在棉花根冠细胞生物测定过程中,棉花黄萎病病菌通过提纯复壮而达到较强的侵染能力,并制备出较多的黄萎病菌粗毒素。介绍了粗毒素的简易制备、根冠细胞培养与制备过程以及染色过程中染色剂的选择、染色时间的长短。实验与传统方法相比有几点改进:总结出细胞振荡时间在1~2 min之内;之后黑暗条件下静置培养2~6 h;选用pH为6.5的0.01%中性红染色3~5 min,之后加入一滴0.2%伊文思兰染色,伊文思兰的染色时间应严格掌握在3min以内等,为根冠细胞测定方法的推广提供了一定的理论支持。 相似文献
109.
两种菊酯类农药对鲤血细胞的影响 总被引:2,自引:0,他引:2
采用对鲤肌肉注射不同浓度的高效反式氯氰菊酯和功夫菊酯,研究了两种菊酯类农药对鲤血细胞形态、红细胞渗透脆性的影响。结果显示:随两种农药攻毒浓度增大、时间延长,鲤血细胞异形率增大。低浓度(6、60μg/kg)中毒鲤成熟红细胞大小不一,核稍有畸形,淋巴细胞、单核细胞增多,核浆界限不清,有空泡,甚至伴有核溶解的白细胞;而高浓度(600、6 000μg/kg)中毒鲤血细胞中除伴有低浓度中毒症状,还出现了染色质模糊,部分白细胞核浆界限不清,甚至溶解,退化更明显。红细胞渗透脆性亦随攻毒浓度增大、时间延长而增高,且开始溶血点的变化幅度较完全溶血点大,表明最小抵抗值更易受环境因子影响。 相似文献
110.