Epidermotropic Cutaneous T-Cell Lymphoma (mycosis fungoides) in Syrian Hamsters (Mesocricetus auratus). A Report of Six Cases and the Demonstration of T-Cell Specificity

Abstract— Epidermotropic cutaneous T-cell lymphoma was diagnosed in six Syrian hamsters (Mesocricetus auratus) with a generalised dermatosis characterised by pruritus, alopecia and exfoliative erythroderma. Plaques and nodules were evident in one animal. Clinical and histopathological findings were suggestive of mycosis fungoides and electron microscopical investigations in one case were supportive. Immuno-histochemical stains for CD3 antigen were strongly positive in all cases. Résumé— Un lyphome T cutané a été diagnostiqué chez six hamsters de Sibérie (Mesocricetus auratus) avec une dermatose généralisée, caractérisée par un prurit, une alopécle et une érythrodermie exfoliative. Des plaques et des nodules ont été observées sur un animal. Les données cliniques et histopathologiques étaient évocatrices d'un mycosis fungoïdes et les examens en microscopie électronique étaient compatibles dans un cas. Les marquages immuno-histochimiques de l'antigène CD3 étaient très positifs dans tous les cas. Zusammenfassung— Bei sechs Syrischen Goldhamstern (Mesocricetus auratus) wurde ein epidermotropes kutanes T-Zell-Lymphom mit einer generalisierten Dermatose diagnostiziert, die durch Pruritus, Alopezie und eine exfoliative Erythrodermie gekennzeichnet war. Bei einem Tier traten auch Plagues und Knoten auf. Die klinischen und histopathologischen Befunde deuteten auf Mycosis fungoides hin. Elektronenmikroskopische Untersuchungen unterstützten diesen Verdacht in einem Fall. Immunhistochemische Färbungen auf CD3-Antigen waren bei alien Fallen stark positiv. Resumen En un grupo de seis hamsters de siria, (Mesocricetus auratus), se diagnosticó limfoma epidermotrópico cutáneo de linfocitos T, presentando una dermatosis generalizada de tipo pruriginoso, acompañada de alopecia y eritroderma exfoliativo. Las placas y nódulos fueron evidentes en uno de los animales. Los estudios clinicos e histopatológicos indicaron una micosis fungoide, y las investigaciones de microscopía, en uno de los caseos, pareció indicar lo mismo. La tintura inmuno-histoquimica para el antígeno CD3 resultó claramente positiva en todod los casos.     

秦岭羚牛角结构与功能的研究

通过对秦岭羚牛的野外观察发现,秦岭羚牛头上长有2只洞角,在与雄性同类个体争偶打斗时,角的使用方式与非争偶的打斗不同;在争偶打斗时,角的前缘具有瞄准对方的作用,通过分析计算羚牛角的前缘也证明了不争偶的雌性与争偶的雄性此处存在差异。     

Interactive Effects of CO2 and O3 on the Growth of Trisetum flavescens and Trifolium pratense Grown in Monoculture or a Bi-Species Mixture

SUMMARY

Golden oat grass (Trisetum flavescens L.) and red clover (Trifolium pratense L.) were grown as monocultures or bi-species mixtures under controlled conditions and exposed to ambient (350 ppm) or elevated (580 ppm) CO₂, with or without addition of O₃ (diel profile with 150 ppb maximum). Shoot biomass measurements after the initial growth and two re-growth periods were used to determine the specific responses of both species, and the difference in the specific response between monocultures and mixtures. T. pratense was much more responsive to CO₂, O₃, and their combination, compared to T. flavescens. In the case of O₃ but not of CO₂, the difference in sensitivity between species was larger in mixture than in monoculture. In contrast to elevated CO₂, O₃ significantly reduced the root:shoot ratio in the mixture, which could explain the increasing negative effect of O₃ on clover with progressing harvests.

The relative CO₂ stimulation of T. pratense and of the cumulative mixture shoot biomass was larger in the presence than in the absence of O₃, which was due to an almost complete protection from O₃ stress by elevated CO₂. In the mixture, the fraction of T. flavescens was small and increased during the experiment; this increase was most pronounced with O3, but any change in mixture biomass was dominated by the response of T. repens.

The results confirm that in grass/legume mixtures legumes are most sensitive to elevated CO₂ and O₃, but the magnitude of specific responses depends on canopy structure and of plant development. Elevated CO₂ minimizes the negative impacts of O₃ stress on above- and below-ground plant growth.     

融安滑皮金桔常温贮藏性状的研究

通过滑皮金桔、滑皮变异、普通金桔三种融安本地金桔常温贮藏试验,观察其外观指数 及品尝其风味,研究融安金桔贮藏性状,为金桔果品产后商品化处理和运输提供技术支持。     

Effects of Dietary Protein and Lipid Levels with Different Protein‐to‐Energy Ratios on Growth,Feed Utilization,and Plasma Biochemical Parameters of Dianchi Golden‐line Barbell,Sinocyclocheilus grahami

An 8‐wk feeding trial was conducted to evaluate the effects of varying dietary protein and lipid levels with different protein‐to‐energy (P/E) ratios on growth, feed utilization, and plasma biochemical parameters of Sinocyclocheilus graham. Nine experimental diets were formulated to contain three protein levels (35, 40, and 45%), and each with three lipid levels (4, 8, and 12%), to produce a range of P/E ratios from 71.31 to 98.64 mg protein/kcal. Each diet was randomly assigned to triplicate groups of 25 fish. Fish grew best when fed the diet containing 45% protein and 12% lipid. However, fish fed the diet with 40% protein and 8% lipid had similar weight gain and specific growth rate as those fed the above diet but showed relatively better feed conversion ratio, protein efficiency ratio, and protein and energy retentions. Additionally, plasma total amino acids and blood urea nitrogen contents and γ‐glutamyltransferase, aspartate aminotransferase, and alanine aminotransferase activities in fish fed the diet containing 40% protein and 8% lipid were relatively low among the dietary treatments. These results may indicate that a diet containing 40% protein and 8% lipid with P/E ratio of 84.05 mg protein/kcal would be suitable for the optimal growth, feed utilization, and health of S. graham.     

Management of Bemisia tabaci biotype B with botanical and mineral oils

While the toxic effects of neem, Azadirachta indica A. Juss, on Bemisia tabaci Genn. are well documented, few studies have evaluated other oils. We compared neem, sesame, citrus, castor, vegetable and mineral oils (1% v/v) to a chemical standard thiamethoxam (0.17 g A.I./L) against B. tabaci biotype B life stages on dry bean plants Phaseolus vulgaris L. under screenhouse conditions. Oils and thiamethoxam exhibited low ovicidal activity (<10% egg mortality). However, significant mortality occurred due to the residual activity to 1st instars that emerged from treated eggs. Overall, impacts of egg treatments were greatest for thiamethoxam (77% total mortality for eggs and 1st instars) compared with oils which were statistically similar (22–29% mortality). Larvicidal effect of oils (against 2nd instars) was greater than ovicidal effects. Highest nymphal mortality (>81%) was achieved with castor, sesame, citrus and neem oils, which was significantly greater than for thiamethoxam (65% mortality). Adult whiteflies were exposed to fresh and aged spray residues, rather than being sprayed directly. In this case, comparatively lower efficacy was achieved from oil treatments compared with thiamethoxam. While some mortality was observed from fresh residues of slow drying oils (up to 41% for castor oil), no significant control from any oil residues >3 days old was observed in our tests. The different route of exposure against adults likely reduced the effectiveness of oil treatments which act directly on the cuticle. In trials with viruliferous adult whiteflies exposed to fresh residues, none of the tested products completely prevented transmission of bean golden mosaic virus (BGMV). However, we noted reduced virus severity ratings from plants pre-treated with castor and citrus oil. We conclude that castor, sesame, citrus and neem oils have the potential to be used in whitefly management programs.     

SPF金黄地鼠核心种群的微生物质量控制

SPF金黄地鼠微生物质量控制对保持动物种群的质量稳定性十分重要。介绍了SPF金黄地鼠核心种群微生物质量控制的具体做法和经验体会,以期为提高隔离器饲养条件下SPF金黄地鼠的种群质量稳定性提供参考和借鉴。     

观赏榆雄孔雀树栖蹲坐造型技艺

挑选2 点高位嫁接金叶垂榆成活1 个接穗的种苗,将白榆和金叶垂榆联合养育成上耸下垂的双色树冠,得到观赏榆作为毛胚树,着眼于成年雄性蓝孔雀整体面貌,抓住蹲坐在树上休息的典型动作,依附孔雀上身金属模具,由中部至上、下两端进行立体加工,采用修剪绑扎技术制作造型,利用绿色叶的白榆创造雄孔雀玲珑的上身,利用金叶垂榆创造雄孔雀硕大的下体,培育出观赏榆雄孔雀树栖蹲坐造型,配植于自然式园林的重要空间,仿佛蓝孔雀入驻植物园。     

金潮原因种铜藻外源无机碳利用特征初步研究

为揭示铜藻(Sargassum horneri)对无机碳利用的基本特性及铜藻在漂浮过程中生物量迅速累积并最终发展为金潮的生理生态学机理,以我国近海金潮原因种铜藻为研究对象,在不同pH (6.5、8.0和9.5)条件下研究了添加TRIS缓冲剂、碳酸酐酶抑制剂(AZ、EZ)和阴离子交换蛋白抑制剂(DIDS)后藻体的固碳速率、光合作用-无机碳响应曲线(P-C曲线)及pH漂移曲线。结果显示,TRIS缓冲剂对铜藻的固碳速率无显著影响(P>0.05),而3种抑制剂均显著(P<0. 05)抑制铜藻的固碳速率且抑制效率从大至小依次为DIDS> EZ>AZ。藻体的固碳速率随着外源无机碳浓度的增加而增加,并渐趋饱和。此外,藻体的最大固碳速率(V_max)随着pH的升高而降低,而半饱和常数(K_0.5)则随着pH的升高而升高,藻体的pH补偿点为9.0左右。结果表明,铜藻既可以利用海水中的CO_2也可以利用HCO_3^-进行光合作用;并且铜藻对HCO_3^-的吸收存在通过阴离子交换蛋白直接转运吸收和利用碳酸酐酶将HCO_3^-转化为CO_2后再吸收两种方式。对HCO_3^-的高效吸收利用能力可能是铜藻保证维持较高光合作用、快速积累生物量并最终演变为金潮的重要因素之一。     

A natural infection by the red sea bream iridovirus‐type Megalocytivirus in the golden mandarin fish Siniperca scherzeri

An outbreak of a Megalocytivirus infection was found in the golden mandarin fish Siniperca scherzeri during September and October 2016, in Korea. Phylogeny and genetic diversity based on the major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes showed a new strain. Designated as GMIV, this strain derived from the golden mandarin fish was suggested to belong to the red sea bream iridovirus (RSIV)‐subgroup I. Additionally, this train clustered with the ehime‐1 strain from red sea bream Pagrus major in Japan and was distinguished from circulating isolates (RSIV‐type subgroup II and turbot reddish body iridovirus [TRBIV] type) in Korea. The infection level, evaluated by qPCR, ranged from 8.18 × 10² to 7.95 × 10⁶ copies/mg of tissue individually, suggesting that the infected fish were in the disease‐transmitting stage. The diseased fish showed degenerative changes associated with cytomegaly in the spleen as general sign of Megalocytivirus infection. The results confirm that the RSIV‐type Megalocytivirus might have crossed the environmental and species barriers to cause widespread infection in freshwater fish.