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71.
黄色镰刀菌Fusarium culmorum为黑龙江省马铃薯干腐病主要致病菌,干腐病可以导致马铃薯在窖藏过程中发生腐烂,影响薯块的商品价值和食用价值,为进一步研究马铃薯干腐病的发生和防治,本研究采用黄色镰刀菌对不同抗性的马铃薯块茎进行侵染,对病原菌侵染过程中薯块的抗氧化酶及细胞壁降解酶变化及病程相关基因的表特性进行了研究。结果表明,当黄色镰刀菌侵染块茎时,块茎中的可溶性蛋白和丙二醛(malondlaldehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)活性、几丁质酶(chitinase)和β-1,3-葡聚糖酶(β-1,3-glucanase)活性都呈现不同程度的上升趋势。非特异性脂质转移蛋白基因StLTPa1表达量随着黄色镰刀菌的侵染时间呈波动性,在植物防御反应中发挥一定的作用。 相似文献
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AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells. 相似文献
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卵巢性生殖疾病发生的主要原因之一是卵子发生过程中减数分裂发生或染色体分离异常。为了更好地进行这些生殖疾病的诊断和治疗,需要充分地了解个体发育的生物学过程中及关键时间点。鉴于此,本文主要阐述了哺乳动物生殖细胞早期发生和减数分裂启动的生理过程,介绍生殖细胞的出现、迁移、性别分化和减数分裂的整个过程。深入了解卵母细胞发生过程将为利用体外发育来源的卵母细胞治疗卵巢性生殖疾病,克服女性不孕、卵巢早衰等重大疾病提供一定的理论支持。 相似文献
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Monika Jamioł Jacek Wawrzykowski Marta Kankofer 《Reproduction in domestic animals》2021,56(7):1040-1049
One of the most important processes determining the proper course of gestation and its physiological termination in cows is the adhesion of epithelial cells allowing for direct contact of maternal and foetal parts of the placenta. Throughout pregnancy, placental cells are under strict hormonal control, which among others regulates the concentration and activity of specific proteins participating in the extracellular matrix remodelling of foetal membranes. The aim of the study was to evaluate the influence of progesterone and prostaglandin F2α on the adhesion of epithelial cells at early-mid pregnancy in cows. Additionally, the impact of selected hormones on anti-adhesive properties of decorin was evaluated. Caruncular epithelial cells were isolated from healthy cows during pregnancy, immediately after slaughter. Primary cell cultures derived from the 2nd and 4th month of gestation were used in the experiments. The viability of cells was assessed by MTT assay. The adhesion of cells to fibronectin was measured spectrophotometrically. The activity of metalloproteinases was confirmed by the metalloproteinase assay. Progesterone (10–5 and 10–7 mol/L) and prostaglandin F2α (10–4, 10–5 and 10–7 mol/L) increased the viability of bovine caruncular epithelial cells in the 2nd month of pregnancy. The treatment with prostaglandin F2α significantly reduced the number of adherent cells from the 2nd month of gestation at the doses of 10–4 and 10–5 mol/L. Both progesterone and prostaglandin F2α were shown to have an effect of decorin resulting in both a decrease in metalloproteinase activity and an increase in adhesion of cells to fibronectin. 相似文献
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GUO Yuan LIU Zong-zheng LIU Le MENG Fan-hua ZHANG Yan-ru ZHOU Huan-min LIU Chun-xia 《中国畜牧兽医》2016,43(11):3011-3018
In order to obtain the cloned sheep using bone marrow mesenchymal stem cells(BMSCs)as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son(RCP)was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment. 相似文献
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YUE Yuan CHEN Hong-yan WANG Jia-wei XU Ming-qiang DING Yu JIANG Hao GAO Yan ZHANG Jia-bao YAN Shou-qing 《中国畜牧兽医》2016,43(3):585-591
The aim of this study was to investigate the differential expression genes induced by ApoCⅢ,and study the function of ApoCⅢ.Porcine aortic vascular endothelial cells were successfully isolated using enzyme digestion,and then screened the differential expression genes induced by ApoCⅢ using the Solexa high-throughput sequencing technology.The results showed 647 differential expression genes,including 390 up-regulated genes and 257 down-regulated genes.The qRT-PCR results verified that the gene expression results from Solexa sequencing data were reliable.GO and Pathway analysis showed that the function of differential expression genes were related to immune response,cell apoptosis and death.These findings suggested that ApoCⅢ affected the physiological function of porcine aortic endothelial cells by the molecular pathways of inflammation,cell adhesion and apoptosis,which provided a theoretical basis for further understanding the molecular mechanisms of atherosclerosis caused by ApoCⅢ. 相似文献
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以“玫瑰红龙”火龙果成熟胚诱导的愈伤组织为材料,研究不同光质配比的LED光处理对火龙果愈伤组织生长的动态影响。结果表明1红1绿光质配比,12h/d照射,最有利于火龙果愈伤组织的生长,在28d至42d细胞增长最快且活性最高,为0.78-0.75。其次是白光>1红1蓝>1绿1蓝>1红1绿1蓝>红光,在70d培养周期内,第35d是较理想的收获时间。 相似文献