RP-HPLC测定贯叶连翘中的金丝桃素含量

采用反相高效液相色谱法测定了贯叶连翘提取物金丝桃素浸膏中金丝桃素的含量。用Kromasil C18(5μm,250×4.6 mm)色谱柱为固定相,乙腈-水(140∶10)为流动相,流速为1 mL/min,检测波长为284 nm。结果金丝桃素的含量为0.914%,平均回收率为96.76%,日内精密度RSD为2.67%,日间精密度RSD为3.65%。     

晋南地区黄瓜、黑籽南瓜死秧的病原菌鉴定及品种抗性研究

经分离、培养对不同菌种培养性状的观察,确定了侵染黄瓜、黑籽南瓜造成死秧的镰刀菌主要为尖镰孢菌黄瓜专化型、尖镰孢菌西瓜专化型、串珠镰刀菌和腐皮镰孢菌4种。经致病性测定,4种镰刀菌均能侵染黄瓜,引起发病造成死秧,可分为强致病类型和中强致病类型。经抗病性鉴定,黑籽南瓜种子只有南瓜4号为耐病品种;黄瓜种子也只有津优31号为耐病品种。     

蛋白质免疫印迹快速测定猪肺炎支原体抗原含量

为建立快速测定猪肺炎支原体抗原含量的方法,试验应用P46单抗建立不同CCU含量的猪肺炎支原体抗原蛋白免疫印迹图谱,测定不同批次的猪肺炎支原体抗原CCU含量,并与Western blot(WB)测定的CCU进行比较.结果 显示:曝光5s,107 CCU/mL含量以上的猪肺炎支原体抗原在46 kDa处有条带,且条带粗细、明...     

高效液相色谱法检测鸡可食性组织中的磺胺氯吡嗪残留

对建立的一种鸡组织中磺胺氯吡嗪钠残留检测的高效液相色谱法进行复核验证。鸡组织样品中磺胺氯吡嗪钠残留经乙腈提取,正己烷去脂肪,MCX固相萃取小柱净化。采用高效液相色谱紫外检测器检测,流动相为乙腈-0.02mol/L磷酸二氢钾水溶液(35:65,V/V),紫外检测波长为272nm,外标法定量。结果显示,磺胺氯吡嗪钠标准工作液在0.04~20.0μg/mL范围内具有良好的线性关系(R^2=0.9999)。在鸡肌肉、肝脏、肾脏、皮脂各组织中添加浓度分别为40、100、200μg/kg时,各组织样品中磺胺氯吡嗪钠的回收率范围为74.70%~93.67%。磺胺氯吡嗪钠的检测限为20μg/kg,定量限为40μg/kg。复核验证的鸡组织中磺胺氯吡嗪钠残留检测高效液相色谱法可行,方法简便快捷,回收率高,适合鸡组织中磺胺氯吡嗪的残留检测。     

Reduction of clubroot (Plasmodiophora brassicae) formation in Arabidopsis thaliana after treatment with prohexadione‐calcium,an inhibitor of oxoglutaric acid‐dependent dioxygenases

Recently, flavonoids were shown to modulate the outcome of clubroot development in Arabidopsis thaliana after infection with the obligate biotrophic pathogen Plasmodiophora brassicae. Therefore, the development of clubroot disease was investigated in Arabidopsis after treatment with prohexadione‐calcium (ProCa), an inhibitor of ascorbic acid/2‐oxoglutaric acid‐dependent dioxygenases such as flavanone‐3‐hydroxylase. The treatment resulted in a reduction of the flavonols quercetin and kaempferol in clubroots, whereas the precursor naringenin highly accumulated. The root system of ProCa‐treated plants was better developed although galls were still visible. Thus, ProCa treatment resulted in reduced gall size. Flavonoids are thought to inhibit polar auxin transport by modulating auxin efflux carriers. It was investigated whether the auxin response might change as a consequence of the accumulation of naringenin in ProCa‐treated plants. In the areas of gall development an auxin response was indicated by the auxin‐responsive promoter DR5 coupled to the reporter β‐glucuronidase (GUS), whereas very little staining was found in healthy root parts. No differences in GUS activity were found between P. brassicae‐infected and ProCa‐treated plants, and plants only infected with P. brassicae, indicating that the effect of ProCa treatment on clubroot reduction is not via changes in auxin responses. As ProCa is also an inhibitor of late steps in gibberellin biosynthesis, a specific gibberellin biosynthesis inhibitor, chlormequatchloride (CCC), was tested on club development. However, CCC did not reduce disease symptoms, indicating that the observed reduced gall development was not because of gibberellin biosynthesis inhibition by ProCa.     

Characterizations and molecular mapping of a novel dominant semi-dwarf gene Sdd(t) in rice (Oryza sativa)

By nitrogen ion implanting, we obtained a semi‐dwarf mutant from a japonica rice cultivar Y98148, designated as Y98149. The genetic analysis of Y98149 indicated that the semi‐dwarf phenotype was controlled by a single dominant gene, Sdd(t). We show that Y98149 reduced plant height mainly via inhibiting the first, second and third internode elongation. Based on this dwarfing pattern, the mutant could be grouped into dn‐type dwarf defined by Takeda [Gamma Field Symp. (1977) Vol. 16, PP. 1–18] . In addition, the Sdd(t) gene was sensitive to gibberellin (GA) based on the response to extraneous GA₃ and the quantitative determination of endogenous GA₁ and GA₄. To map the Sdd(t) gene, we tested molecular markers by bulk segregant analysis. The Sdd(t) gene was localized to a 6.4 cM‐interval on the short arm of chromosome 6, flanked by two sequence‐tagged site markers S9 and S13.     

苹果蠹蛾老熟幼虫蛹期有效积温法则预测相关因子分析报告

通过对苹果蠹蛾相关资料认识及对相关因子的计算得出对相关因子的预测.     

牛奶中环丙沙星残留的微生物学检测方法研究

以藤黄微球菌(标准号28001)为工作菌,用圆滤纸片法测定环丙沙星在牛奶中的残留.结果表明,环丙沙星在牛奶中的最低检测限为 0.08 μg/mL.该方法精密度良好,变异系数小于15%,操作简便,不需特殊设备,样品用量少,易于推广,适用于环丙沙星的半定量分析.     

禁限用农药隐性添加的检测与风险分析研究

禁限用农药隐性添加是目前影响农药产品质量和农产品质量安全的重要因素之一。本文利用气-质联用技术,检测了2015年吉林、辽宁、黑龙江、河北、山东、江苏等11个省份农药市场上购买的90个批次样品和网络上购买的10个批次样品中甲胺磷、水胺硫磷、氧化乐果等禁限用农药成分。结果发现,在市场抽检的93个化学农药样品中,禁限用农药成分检出率为3.2%:市场抽检的7个生物农药样品中,禁限用农药成分捡出率高达42.9%表明,生物农药隐性成分添加已经成为其市场上的主要问题     

口蹄疫病毒Hankou/99株基因组全序列测定与基因特征分析

本研究主要对口蹄疫病毒(FMDV) Hankou/99株全基因序列进行测定,通过基因序列分析,确定其基因型,丰富了FMDV基因库,为研究猪源FMDV的分子变异、感染性分子克隆及致病机理奠定基础.从感染FMDV Hankou/99株的细胞液中提取RNA,通过RT-PCR技术,获得猪FMDV Hankou/99分离株覆盖全基因组的5个cDNA片段(S、L、C、D、E),分别对这些片段进行克隆和序列测定.结果显示,FMDV Hankou/99株全基因组长8099 bp;5'NCR长1040 bp,开放阅读框长6966 bp;3'NCR长93 bp,其后是30个碱基的连续poly(A)结构.通过与参考株基因组结构比较分析,显示其在分类地位上属于O型FMDV,并与猪源FMDV毒株OLZ、TW/97同源性较高,特别是3A区域上都有30 bp的缺失.另外,通过与9个参考株的VP1系统发生树分析,显示其与OLZ、TW/97、O/Akesu/58、O/OMⅢ 4个毒株为同一基因型.