Genetic diversity of apricot revealed by a set of SSR markers from linkage group G1

Eight polymorphic simple sequence repeat (SSR) markers located in the G1 linkage group of apricot (Prunus armeniaca L.) were previously developed and evaluated in a small set of cultivars. Those primers were used for studying variability in 77 apricot cultivars belonging to five different geographical groups, such as Chinese, Asian (Irano-Caucasian and Central Asian), North American, Mediterranean and Western European as well as Middle European cultivars. Six of the markers were polymorphic and revealed a total of 71 alleles ranging from 5 (aprigms11) to 20 (aprigms1) alleles per locus with a mean value of 11.83 alleles per locus. In conclusion, the SSR loci located in the G1 linkage group show a level of polymorphism which is similar to loci dispersed throughout the entire genome. The total number of alleles and the number of unique alleles were the highest in Chinese apricots and the lowest in Middle European cultivars. Heterozygosity also showed a decrease from Asia and China to Middle Europe. No association could have been observed between any SSR markers tested and plum pox virus (PPV) resistant phenotype of cultivars. PPV resistant cultivars did not form a separate clade on the dendrogram obtained by UPGMA cluster analysis. Middle European and Chinese cultivars formed separate clusters while other genotypes formed smaller multiple sub-groups or scattered among different clusters. Our results support previous hypotheses on the origin of PPV resistance in North American apricots. The allele data was also presented in a form that allowed the easy observation of allele frequencies in each geographical group at each locus. Using this data field, differences and similarities between cultivar groups can be easily assessed. The analysis demonstrated the links between the North American and Mediterranean apricot germplasm and confirmed that the Chinese and Eastern European cultivars are distantly related.     

汛期横江入流对向家坝一期围堰河段水位的顶托影响

为保证向家坝一期围堰工程汛期安全度汛,时汛期横江入流的向家坝一期河段水位的顶托影响进行了研究.研究时首先采用实测资料对横江洪水的顶托影响进行分析,在此基础上,采用BP神经网络对不同横江来流下向家坝水文站的水位变化进行预测,给出向家坝水文站的水位壅高幅度.结果表明:横江洪水对向家坝河段水位顶托影响主要发生在金沙江洪水过程的涨水段,一期围堰修建后向家坝水文站受横江来水顶托影响主要发生在屏山流量为8 OOO～17 000 m3/s,且横江流量大于1 000m3/s时的范围内,其水位壅高幅度一般在0.44～1.5 m范围内变化.     

区域尺度土壤入渗特征参数的变异性研究

以杨凌地区为例,进行野外双环入渗试验研究,运用2种常用模型(Kostiakov模型和Philip模型)对土壤入渗参数进行模拟。在分析土壤特征参数,寻求适合区域尺度的最优入渗模型同时,对土壤的状态变量进行标定并研究标定系数的空间变异性,研究结果表明:Philip模型可作为区域最优入渗模型,土壤入渗特征参数存在明显的空间变异性,标定系数aA比as对模型参数的标定效果好,且在较大范围内存在一定的趋势性,有较好的预测效果。因此,可根据标定系数随机模拟得到某一具体时刻的土壤入渗量的空间分布,为定量分析土壤水分运动、研究土壤水分入渗的空间分布提供理论基础。     

基于回归等值线法的不同下垫面红壤水分时空变化规律研究

基于回归等值线法对百喜草覆盖、百喜草敷盖和对照处理的红壤水分时空变化进行了比较分析。结果显示,从年初到年终,随时间的推移,各处理各层土壤含水量均表现出"先减小,后增大"的趋势。在测定深度内,随土层深度增加,各处理各层土壤含水量却表现出"先增大,后减小"的趋势。百喜草覆盖处理,特别是上中层土壤含水量,随时间变化较大,与气候和百喜草生长密切相关,但随深度变化,层间土壤含水量变化较慢;百喜草敷盖和对照处理,土壤含水量受土层深度因素影响更明显。对于百喜草敷盖处理,随深度变化,层间土壤含水量变化较慢,但对于对照处理,随深度变化,层间土壤含水量变化较大。     

Genetic variations in survival of rohu carp (Labeo rohita, Hamilton) after Aeromonas hydrophila infection in challenge tests

A challenge test against Aeromonas hydrophila was conducted using 2062 rohu carp (Labeo rohita) fingerlings obtained from 52 dams and 87 sires (87 full-sib families) of two year-classes (2003 and 2004). Attempts to establish a cohabitant challenge model were not successful. Therefore, fish were challenged by intraperitoneal injection with A. hydrophila in two replicate tanks per year-class, and dead fish were collected hourly. The mortality reached its peak at 16–22 h after challenge and had almost completely stopped after 58 h. The test was terminated after 382 h at which the average survival was 43.9 and 48.9% in the two 2003 year-class tanks, and 77.1 and 35.8% in the two 2004 year-class tanks. Heritability estimates for survival were obtained from sire and dam threshold models (THR) and sire and dam linear repeatability models (LINR). For both types of models the heritability estimates based on data from single tanks were not consistent. For both year-classes, data from one of the two challenged tanks demonstrated significant additive genetic variation in survival during the A. hydrophila infection, whereas the heritability estimates were not significantly different from zero for the other tank. Further, genetic correlation between survivals in the two replicate tanks in each year-class was not significantly different from zero. The differential results from the replicate tanks demonstrate that additional challenge test experiments are needed before firm conclusions can be drawn about the magnitude of additive genetic variation for survival to aeromonasis in rohu carp. A cohabitant challenge model that allows the testing of important defence mechanisms in the skin and mucous membranes of the fish might have been more appropriate. To establish a valid cohabitant challenge model for rohu carp should be given high priority.     

A rapid extensigraph protocol for measuring dough viscoelasticity and mixing requirement

The extensigraph is particularly useful in characterizing dough viscoelastic properties; however, testing throughput for standard method is low due to the prerequisite for farinograph water absorption, long dough resting and milling to prepare large amounts of flour. Therefore, a rapid extensigraph method was developed that reduced sample size (165 g wheat) for milling and more than tripled throughput. Wheat is milled in Quadrumat Junior mill with a modified sieving system. The resulting flour (100 g) was mixed with a pin mixer at constant water absorption to allow the evaluation of wheat genotypes at the absorption level they are expected to perform. Dough was subsequently stretched by an extensigraph after 15 min of floor time and 30 min resting. Strong correlations for extensigram R_max (r > 0.93), extensibility (r > 0.64) and area (r > 0.88) were found for the proposed method compared to the standard method. Mixing parameters (time and energy) obtained during dough preparation provided further information about dough strength and mixing requirement. By significantly reducing sample size requirement and increasing testing throughput, this rapid extensigraph method can be widely adopted in milling and baking industry and meets the need for a fast evaluation of dough strength in breeding trials.     

甘肃小麦品种主要春化和光周期基因的组成和分布

为了明确甘肃小麦春化和光周期基因的分布特点,利用STS标记对96份品种的主要春化基因位点VRN-A1、VRN-B1、VRN-D1、VRN-B3和光周期基因PPD-D1位点的等位变异组成进行了检测和分析。结果表明,在甘肃小麦品种中,春化和光周期基因等位变异组合存在11种类型,每种类型的分布频率不同。其中,Ppd-D1a类型频率最高,Vrn-A1/Vrn-B1/Ppd-D1a次之。在春麦生态区存在11种组合类型,其中VrnA1/Vrn-B1/Ppd-D1a频率最高,Ppd-D1a次之。在河西灌溉春麦区、中部干旱春麦区与洮岷高寒春麦区频率最高的组合类型分别为Vrn-A1/Vrn-B1/Ppd-D1a、Vrn-A1/Vrn-B1和Ppd-D1a。与春麦生态区相比,冬麦生态区不存在春化基因显性变异Vrn-A1,且仅存在Ppd-D1a、Vrn-B1/Ppd-D1a、Vrn-D1/Pp-D1a三种类型的等位变异组合,其中,Ppd-D1a类型频率最高,Vrn-B1/Ppd-D1a次之。在陇南冬麦区、渭河上游冬麦区、泾河上游冬麦区中,基因组合类型Ppd-D1a均占主导地位,分布频率依次为46.2%、93.6%和100%。     

伊犁察南灌区土壤盐分时空变异特征与运移机理研究

以伊犁河谷察南灌区为研究区,运用磁感式电导率快速获取技术及室内分析相结合手段,通过构建盐分解译模型,获取2015年及2016年秋季0~30 cm、30~60 cm、60~100 cm土壤盐分含量。对土壤各土层进行了盐渍化分级统计和半方差函数分析及空间插值分析。对研究区高程信息进行提取,并通过调查河流影响范围对河流进行缓冲区分析。旨在构建适用于研究区及同类型灌区磁感式盐渍土地快速精量诊断评估与利用规划技术体系,了解干旱、半干旱区灌溉农田土盐时空变异特征,并对土盐时空运移机理进行研究。初步研究表明,相比于2015年秋季,2016年秋季研究区非盐化土面积显著减小,中度盐化土面积显著增加,非盐化土、轻度盐化土呈现向中度盐化土演变的趋势;研究区土壤盐分呈现向中部及东部区域运移的趋势,盐化土面积增加,且盐分含量均值增大;另外,在空间大尺度范围上,河流是影响土盐运移的主要影响因素,在局域小尺度范围内,地势是影响土盐运移的主要影响因素。     

西安气温日变化特征的城郊差异

为了研究城市化对西安气温日变化的影响,利用西安及其郊区站咸阳1961—2013年4次气温的观测资料,采用城郊对比法对西安和咸阳2个站气温的年、季节、6 h变温、昼夜温差的变化特征进行对比分析,揭示城市化对西安气温日变化特征的影响。结果表明:(1)西安市气温的变化无论哪个时次都高于咸阳且呈上升趋势,2时西安气温的上升幅度最显著,0.56℃/10 a,14时西安与咸阳气温变化趋势基本一致;(2)西安城市气温上升变化幅度从1990s中期开始明显增大,且城市季节变化趋势最明显的是春季,其次是冬季,夏季变化最小,咸阳气温变暖趋势明显滞后于西安5~8年;6 h气温变化温幅度最大的是早晨到中午这个时段,最小的是夜间到早晨。     

1961—2015年安徽省春季日照时数变化特征

为了研究安徽省春季日照时数变化特征,为合理安排农事提供依据,使用近55a(1961—2015年)安徽省春季78个气象台站逐日日照时数资料,利用相关分析、线性趋势分析、Mann-Kendall以及Morlet小波变换分析等方法对安徽省春季日照时数进行了分析,结果表明：安徽省春季多年平均日照时数在381.55—626.75h之间,呈北多南少的空间分布特征;春季日照时数年际变化明显,日照时数最多年为最少年的1.7倍。在近55a中,日照时数整体上呈现出先减后增的变化趋势;在各个年代中日照时数周期存在差异;在21世纪初日照时数由减少转为增加,属于突变现象;安徽省春季日照时数变化趋势存在明显的空间差异,淮北主要以减少趋势为主,江淮之间增减参半,沿江江南则主要表现为增加趋势。