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Three trickle irrigation schedules, two of which were scheduled according to soil water potential ( soil) (tensiometer method) and daily stem contraction (DSC) (dendrometer method) respectively and the other one was a schedule of restricted water supply, were applied to a mature peach orchard.The annual water application based on soil was greater than that based on DSC. However, tree growth, fruit size and leaf water potential (leaf) on the trees in the dendrometer scheduling plot did not differ from those in the tensiometer scheduling plot while the premature fruit drop and fruit bud initiation were greatly different. The restricted water supply treatment limited significantly both tree and fruit growth. In addition, the lower leaf was observed on the trees in this plot.Further study shows that use of the dendrometer method for scheduling irrigation satisfies the water needs of the plant and that the tensiometer method is less accurate.Abbreviations leaf
leaf water potential
- soil
soil water potential
- DSC
daily stem contraction
- LVDT
linear variable displacement transducer
- PET
potential evapotranspiration 相似文献
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AIM To construct the mouse embryonic stem cell (ESC) line with stable pancreatic and duodenal homeobox 1 (Pdx1 ) expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1+ definitive endoderm cells into pancreatic cells. METHODS The Pdx1 -overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups: blank control group (ESC group), empty lentivirus control group (PDX1- ESC group) and Pdx1 lentivirus transfection group (PDX1+ ESC group). Flow cytometry was used to detect the transfected cells after screening by doxycycline (DOX). The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1- ESC group and PDX1+ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS (1) The positive rates of transfected cells in PDX1- ESC group and PDX1+ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1- ESC group and PDX1+ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. (2) With DOX, green fluorescence was observed in PDX1- ESC group and PDX1+ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1+ ESC group (P <0.05). Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed (P >0.05). (3) After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1+ definitive endoderm cells into pancreatic cells. 相似文献
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红叶石楠离体培养技术研究 总被引:2,自引:0,他引:2
以红叶石楠半木质化带芽茎段为外植体,探讨不同外源激素水平对其不定芽分化、增殖与不定根形成的影响。结果表明:MS BA 1.0~2.0 mg/L IBA 0.1~0.3 mg/L GA 0.5 mg/L的分化增殖效果好;增殖系数达6.5以上;White NAA0.1 mg/L AC(活性炭)0.5%或White IBA 0.1 mg/L AC 0.5%或White IAA 0.5 mg/L AC 0.5%的生根效果较好,生根率可达90.5%以上;在珍珠岩∶蛭石∶腐叶土=5∶2∶1的基质中炼苗,成活率可达96%以上。 相似文献
67.
以软枣猕猴桃无芽茎段为外植体进行离体培养,具有成本低、取材方便、材料充足等优点,但以往的研究表明无芽茎段诱导再生率低。本试验以‘奇异莓6号’软枣猕猴桃无芽茎段为外植体,探究不同植物生长调节剂、光照强度、温度、苗龄对不定芽诱导的影响。结果表明:最佳外植体为苗龄30d的无芽茎段,在光照12h/d(光照强度50LX)、室温20℃下,不定芽诱导的最适培养基为MS+2mg/L ZT,不定芽以间接途径发生,培养40d无芽茎段两端开始形成淡绿色愈伤组织,培养45d愈伤组织表面出现红色小点,开始形成不定芽,培养55d愈伤组织诱导率、愈伤组织分化率及不定芽数均到最大,分别为100%、100%及18.87个/块。待苗长至3cm左右时转到MS+0.4mg/L IBA中进行生根培养,培养30d生根率可达100%、根数为8.53/株、根长1.68cm、根粗0.14cm。 相似文献
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为了提高西番莲扦插苗的成活率和成活质量,本研究以黄壤、黄壤:有机肥=1:0.05为扦插基质,以0 mg/L、100 mg/L、200 mg/L为不同梯度的生根粉浓度,探索不同基质和生根粉浓度对西番莲扦插苗成活率、生物学特性及生物量的影响。研究表明:在不同基质条件下,以黄壤:有机肥=1:0.05配比效果最好,成活率达到93%,生物学特性(主根长、主根数、侧根长、侧根数、侧茎长、叶长、叶宽和叶片数)和生物量(植株重、根重、茎重、叶重)均较黄壤表现好;在不同生根粉浓度梯度下,则表现为200 mg/L >100 mg/L >0 mg/L。说明西番莲扦插繁殖以黄壤:有机肥=1:0.05作为基质,以200mg/L的生根粉浓度处理扦插穗条效果最佳。 相似文献
70.
将黑蒜和山药加入马铃薯粉中,研制烘烤型黑蒜山药薯片。以感官评分和质构特性为评价指标,探讨黑蒜粉添加量、山药粉添加量、白砂糖添加量、食盐添加量以及烘烤温度和烘烤时间对烘烤型黑蒜山药薯片品质的影响。在单因素试验的基础上,通过正交试验优化烘烤型黑蒜山药薯片制作配方及工艺。结果表明,烘烤型黑蒜山药薯片的最佳配方及工艺为:马铃薯粉添加量30 g,白砂糖添加量9 g,油添加量2 g,食盐添加量1.0 g,黑蒜粉添加量1.5 g,山药粉添加量9 g,水添加量50 mL,烘烤温度145℃,烘烤时间22 min。按此配方及工艺条件生产的黑蒜山药薯片质地均匀,无裂纹,口感较好,入口爽脆,有独特的黑蒜风味。研究结果为新型薯片的研制提供技术支持。 相似文献