Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.     

不同剂量25羟基维生素D3对断奶前犊牛血液生化指标、抗氧化能力和免疫功能的影响

为研究不同剂量25羟基维生素D₃[25(OH)VD₃]对断奶前犊牛血液水平、抗氧化能力和免疫功能的影响,选取18 头健康状况良好的新生荷斯坦犊牛,随机分成3 组,每组6 头进行为期56 天的饲养试验。分为对照组[不添加25(OH)VD₃]、低剂量组[25(OH)VD₃添加量6 000 IU/头·天]和高剂量组[25(OH)VD₃添加量12 000 IU/头·天]。结果表明,与对照组相比,随着25(OH)VD₃添加剂量增加,血浆生长激素、胰岛素样生长因子-1、过氧化氢酶、超氧化物歧化酶、总抗氧化能力以及免疫球蛋白G(IgG)含量显著增加(P<0.05),而丙二醛含量随剂量增加而存在降低趋势(P=0.079)。与对照组和低剂量组相比,高剂量组血浆免疫球蛋白A(IgA)含量有增加趋势(P=0.059)。综上所述,断奶前犊牛饲喂高剂量25(OH)VD₃有利于促进体内生长激素和胰岛素样生长因子-1分泌,可提高犊牛抗氧化能力,增强免疫功能。     

援非农业技术示范中心可持续发展途径思考

中国援非农业技术示范中心陆续完成技术合作阶段,并逐步进入可持续发展期。顺利完成这个过渡期,需要前期大量的准备工作,如:中心选址、项目内容确定、专家遴选、项目设计及认真的实施过程等。     

Analysis on Function Orientation and Development Countermeasures of New Agricultural Business Entities

Along with the rapid advance of industrialization and urbanization process, fostering new agricultural business entities become inevitable for agricultural transformation and the construction of agricultural modernization in China. The status of the new agricultural business entities determines the level of modern agricultural development. In recent years, new agricultural business entities have grew rapidly. However, there are still many problems including the difficulties in financing loans, inadequate agricultural insurance system, bad implementation of agricultural subsidies, jagged agricultural talents and so on. In order to foster new agricultural business entities, countermeasures should be carried out to ensure financial support, perfect the agricultural insurance, strengthen the level of agricultural subsidies, strive to develop the degree of specialization agricultural operators and so on.     

白花蛇舌草超微粉对小鼠免疫功能的影响

The assay was aimed to study the effect of Hedyotis diffusa Willd superfines powder on immune function. Three groups of mice were gavaged with the drugs at doses of 1.75,1.25 and 0.75 g/kg once a day for 7 consecutive days, repectively. The mice of control group were administered with the same volume of saline.At the last time, mice were injected into the muscle with Newcastle disease vaccine,and get peripheral blood on days 7, 14 and 21 after the medicine administration. The enhancement of innate immune responses were evaluated by using CCK-8 method, hemagglutination inhibition test, macrophage phagocytic function test and organ coefficient method. The levels of lymphocytes proliferative capacity, serum antibody, peritoneal macrophage phagocytosis and the indexes of immune organs in mice were extremely significantly enhanced (P＜0.01). Hedyotis diffusa dWilld superfines powder could extremely significantly regulate the immunity function of mice (P＜0.01).     

大学图书馆组织结构设计探讨

运用现代组织设计的相关理论,对传统大学图书馆的组织结构进行深入剖析,分析了制约大学图书馆功能发挥的各种因素,提出了现代大学图书馆的组织结构设计方案。该方案能够兼顾图书馆教学、学科建设与科研以及图书馆的基本服务功能。     

水生动物肌醇营养研究进展

肌醇是大多数水生动物保持健康、促进生长发育必需的营养素,它在细胞膜磷脂合成、神经递质传递和肝脏脂质转运等方面发挥重要作用。饲料中肌醇缺乏导致水生动物生长不良、体表充血、鳍条糜烂、体色发黑、脂肪肝等症状。本文综述了肌醇的理化性质,肌醇在水生动物营养中营养生理作用、需要量及影响因素等,同时还介绍了肌醇的来源和测定方法,并展望了肌醇在水生动物营养中进一步的研究和应用方向。     

科技英语疑问句的信息功能探析

句子的功能所实现的交际任务是传递信息。根据话语信息传递和语言交际功能理论原则,通过甄选科技英语相关例证作为分析语料,从英语的科技文体视角探析了疑问句在科技英语信息推进中的五大作用,同时指出了解这些相关作用的必要性。     

枣抑制消减cDNA文库构建与分析

枣树具有独特的开花结果特性,为获得枣花发育基因,以金丝4号枣不同发育时期的花蕾和同时期的叶片cDNA互为试验方和驱动方,利用抑制消减杂交技术分别构建了枣花和枣叶抑制消减杂交cDNA文库(SSH文库)。枣花和枣叶SSH文库重组率分别是92.05%和90.26%,插入片段长度均介于200~2 000 bp之间,主要集中于1 000 bp左右。分别从枣花和枣叶SSH文库中随机挑选1 000个阳性克隆进行DNA测序、组装拼接,结果显示枣花SSH文库中获得96条unigene,其中contigs 43个,singletons 53个,96条unigene预测得到73个ORF,经过同源比对分析获得有注释的序列20条,按功能分为7类;枣叶SSH文库中获得86条unigene,其中contigs 45个,singletons 41个,86条unigene预测得到70个ORF,经过同源比对分析获得有23条注释的序列,按功能分为6类。