基于18S rRNA的鸡组织滴虫病PCR诊断方法的建立

河南某鸡场约4周龄鸡疑似发生鸡组织滴虫病.根据鸡组织滴虫18 S rRNA序列设计引物,提取肝脏、盲肠内客物寄生虫DNA,采用PCR方法检测.结果表明,PCR扩增到与预期大小一致的产物,经测序比对,证实为鸡组织滴虫感染.所建立的PCR方法具有灵敏、特异、快速等优点,不仅能用于鸡组织滴虫病临床诊断,还能用于开展流行病学研...     

仓库管理系统的研究与开发

仓库管理系统是典型的信息管理系统(M IS),其开发主要包括后台数据库的建立和维护,以及前端W eb应用程序的开发两个方面。本研究以VISUAL BASIC作为开发工具,同时用M icrosoftAccess 2003建立后台数据库,最后以JET数据库引擎技术实现前台界面与后台的连接。实践证明,该仓库管理系统能够完成仓库货物信息的录入、查询、统计和数据备份等功能,具有极强的实用性。     

基层农机管理服务工作探讨

就农机管理服务中存在的问题以及如何做好农机管理服务工作提出建议。     

Potential carbon stock in Japanese forest soils – simulated impact of forest management and climate change using the CENTURY model

Forest management and climate change may have a substantial impact on future soil organic carbon (SOC) stocks at the country scale. Potential SOC in Japanese forest soils was regionally estimated under nine forest managements and a climate change scenario using the CENTURY ecosystem model. Three rotations (30, 50, 100 yr) and three thinning regimes were tested: no‐thinning; 30% of the trees cut in the middle of the rotation (e.g. 15 year in a 30‐yr rotation) and thinned trees all left as litter or slash (ThinLef) and the trees from thinning removed from the forest (ThinRem). A climate change scenario was tested (ca. 3 °C increase in air temperature and 9% increase in precipitation). The model was run at 1 km resolution using climate, vegetation and soil databases. The estimated SOC stock ranged from 1600 to 1830 TgC (from 6800 to 7800 gC/m²), and the SOC stock was largest with the longest rotation and was largest under ThinLef with all three rotations. Despite an increase in net primary production, the SOC stock decreased by 5% under the climate change scenario.     

转基因油菜TOPAS 19/2质粒分子多家协同实验及不确定度评定

质粒分子是转基因产品核酸定量检测的一类新型标准物质,具有易制备、周期短、成本低等特点。采用实时荧光定量PCR技术,并协同7家实验室对转基因油菜TOPAS 19/2质粒分子进行了基因组的可替代性研究、协同实验研究及不确定度评定。T检验表明,质粒和基因组所产生的内源和外源基因标准曲线的斜率和线性相关系数没有显著性差异。对多家定值的数据进行了统计分析得出,TOPAS19/2质粒分子的量值结果0.910,扩展标准不确定度（K=2）为0.013。     

Spatial optimization by simulated annealing and linear programming

Forest management planning comprises selection among treatment alternatives in management units. A traditional linear programming (LP) approach may effectively account for a profit maximization objective combined with sustainability constraints, e.g. on the temporal distribution of harvest volume flows, cash‐flow, and net present value development, but it fails to account for spatial constraints, especially those associated with final felling. By applying a simulated annealing adjacency model based on net present value maximization and combined with an LP consequence computation model, it is possible to delineate optimal strategies of final felling scheduling. Evaluation is made of the trade‐off between (1) the incremental cost (determined by use of the LP model) of an optimal adjacency model solution, and (2) the potential damage cost resulting from adjacency characteristics such as windthrow and bark injuries. The decision support system may contribute significantly to reduce damage costs and may improve the reliability of forest management planning.     

项目教学法在高职高专种植类专业教学中的运用

高职高专种植类专业课程的特点必须注重实操性与创新性.为了培养高素质、高能力、复合型人才,要求教师必须不断探索新的教学方法.本文介绍了一种在各国高校其它专业已经成功应用的“项目教学法”,结合作者几年的教学实践经验总结,重点探讨了“项目教学法”在本专业的操作优势和具体实施过程,同时指出该教学法存在的局限性和应注意事项,为在种植类专业各课程中推广“项目教学法”提出一些建设性措施和方法.     

提高玉米产量的“四防六技巧”

玉米在生长过程中如果管理不善，容易出现早衰、空秆、缺粒、倒伏等现象，这样就会严重影响到玉米品质和产量。如何防止这种现象发生，应着重做好“四防”。玉米生长的前期如何做到“促下控上”，中后期为授粉结实创造良好的生长环境，提高光合效率，延长根和叶的生理活性，达到粒重饱满高产的目的，应做好“六技巧”。     

中文期刊阅览管理模式刍议

对图书馆期刊阅览管理模式中存在的矛盾,从实际观察到理性思辩,试行提出解决的措施。     

从个案调查辨析改进院校图书馆管理服务

通过对某专业院校图书馆文献借阅情况调查,就改进院校图书馆管理,优化读者服务谈了一些看法。侧重于研究教学型的专业院校图书馆作讨论。