Effect of ABA,arginine and sucrose on protein content of date palm somatic embryos

Abscisic acid (ABA), arginine and sucrose were evaluated for their effects on the morphology, germination rates and protein content of date palm somatic embryos (SE). Different concentrations of these supplements in the culture medium were used. The comparative study of SE length and thickness between treated and untreated SE revealed no differences, except for ABA (20 μM), which increased thickness. A decrease of water content (WC) in favor of an increase in dry weight (DW) was observed in all treated SE, especially with sucrose (90 g l⁻¹) and ABA (20 μM). Only ABA (20 and 4 μM) caused a proliferation rate of the cultures higher than those in the control. Although all the tested compounds increased protein content, ABA (20 μM) was more effective in protein enrichment than arginine and sucrose treatments. The SDS-PAGE protein profiles showed a significant difference between treated and untreated SE. A protein band of 22 kDa, identified as glutelin in a previous work, was accumulated after treatment with 20 μM ABA or 3 mM arginine. These findings may contribute to further understanding of the mechanisms involved in the accumulation of specific storage proteins in several plants.     

鲜干兼用型辣椒新品种‘天椒五号’的选育

‘天椒五号’是从甘肃甘谷线椒98—15中选择优良变异单株98—15—2,经连续多年定向分离、提纯育成的优良新品种。中熟,植株生长势强,抗逆性强,高抗辣椒疫病、炭疽病。果实浅绿色,长线椒形,中辣,商品性好,品质优良。适宜北方早春大棚及露地栽培。     

Egg quality determination based on the shape of the lipid vesicle in common dentex, Dentex dentex

The shape of the lipid vesicle (the ratio between the maximal and the minimal diameter and the shape coefficient) of the eggs of Diplodus puntazzo and Sparus aurata is correlated with larval survival rate (%) and can be used as an egg quality marker as reported previously by Lahnsteiner and Patarnello (2005) . In the present study, this method for egg quality determination has also been validated for common dentex, Dentex dentex, after introducing several modifications in the calculation and evaluation procedure in order to obtain higher sensitivity. In the present study, it could be demonstrated that the shape of the lipid vesicle is not only correlated with the hatching rate but also with the survival rate of 1, 2 and 3 days post hatch larvae and therefore it can also be used as an indicator of larval viability at early stages of development.     

Effects of silvering state on induced maturation and spawning in wild female Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis>

ABSTRACT:   The effects of silvering state of wild female Japanese eels Anguilla japonica on the success of induced maturation and the following spawning were examined. Thirty-eight females, collected in Mikawa Bay, were divided into four stages based on their silvering state: yellow (Y1), late-yellow (Y2), silver (S1) and late silver eels (S2). Despite injections of salmon pituitary extract (SPE) through the standard technique, Y1 and Y2 eels did not respond to the treatment with undeveloped gonad (gonad-somatic index [GSI]: 0.3–0.9), and all these females died by 5 weeks, probably due to an abnormal physiological condition. Most S1 (81%) and S2 eels (100%) matured completely (GSI: 17.8–51.4), and finally spawned successfully (69% for S1, 89% for S2). S2 eels fully matured with oocytes of over 750 μm in diameter by significantly smaller number of injections of SPE (5–6 times) than the case of S1 eels (6–8 times). The amount of eggs released by S2 eels (0.65 ± 0.11 g/fish per body weight [BW]) was significantly larger than those by S1 eels (0.54 ± 0.09 g/fish per BW). There was no difference in fertilization and hatching rates between eggs released by S1 eels and those of S2 eels. These results indicate that the success of induced maturation and spawning in wild female Japanese eels depends on their silvering state, and matured eggs can be obtained efficiently through the use of S2 eels rather than other stages.     

Inter-river, -annual and -seasonal variability in fecundity of Atlantic salmon, Salmo salar L., in rivers in Newfoundland and Labrador, Canada

Abstract  Fecundity is an integral component of the calculation of Atlantic salmon, Salmo salar L., egg depositions in rivers. Fecundity determinations can be time consuming and prohibitively expensive in terms of application on a broad scale. Consequently, where river specific and annual data are not available, default means are used in calculations in Newfoundland and Labrador. It is important therefore to know the extent of variability among rivers, years and seasons and the potential error involved in using default values. Annual fecundity data were available for one river in Labrador and nine rivers in Newfoundland. Fecundity was determined from ovaries collected in the recreational fishery in the summer for all 10 rivers. For three of these rivers, fecundity determined from summer sampling was compared with that obtained from sampling at time of spawning in autumn. There was significant variability in fecundity with length as a covariate among rivers, years and seasons. Mean number of eggs per female decreased between 8.3% and 29.0% from summer to autumn while mean number of eggs per cm decreased from 5.0% to 28.5%. Depending on the measure of relative fecundity used (no. of eggs kg⁻¹ or no. of eggs cm⁻¹), results of simulations showed that estimates of egg deposition incorporating defaults can deviate from those obtained by applying year-specific and river-specific values by 50–75%, without adjusting for the seasonal reduction in fecundity, and by 30–50% with an adjustment. A sensitivity analysis revealed that of three parameters used in the calculation of egg deposition (size, percent female and fecundity), fecundity was the most influential.     

Impact of sampling and storage technique,and duration of storage,on the composition of fresh grass when analysed using near‐infrared reflectance spectroscopy

The objective of this study was to examine the effect of sampling technique (pluck or cut), storage duration (immediate analysis, 24‐h or 48‐h), storage temperature (ambient or chilled) and storage conditions (air present, air excluded or breathable) on the composition of fresh grass sampled from a sward managed to simulate grazing. Treatments were repeated across four sampling dates, with grass samples stored in grip seal bags prior to analysis using near‐infrared reflectance spectroscopy. Grass sampled by ‘pluck’ had a higher crude protein and ME content, and a lower acid detergent fibre (ADF) content, compared to that sampled by ‘cut’. Grass stored for 48 h had a lower water soluble carbohydrate (WSC) and ME content and a higher ADF content than for immediate analysis. Samples stored for 24 h did not differ from immediate analysis. Grass stored at ambient temperature had a lower WSC and ME content compared to immediate analysis. Grass stored under ‘breathable’ conditions had a lower ME content and higher ADF content than immediate analysis or samples stored with air present or air excluded. It is recommended that grass for analysis should be sampled by cutting, stored chilled (4°C) in a sealed bag to minimize exposure to oxygen and analysed within 24 h of harvest.     

基于SWOT分析的我国生鲜电商物流研究

随着网络的普及,网购群体越来越庞大,对生鲜产品的需求也越来越大,但是我国的冷链物流还处于初级阶段,通过SWOT分析法对我国生鲜电商物流的优劣势、面临的机遇和挑战进行分析,并提出相应的对策,为促进我国生鲜电商物流的发展提供参考借鉴。     

鲜食枣树体根部矿质元素含量的年动态变化

为了研究枣树根系年生长周期内矿质元素含量变化的情况,提高枣园的栽培管理技术,为科学合理的施肥提供一定的科学依据。对‘大白铃枣’、‘大瓜枣’、‘梨枣’、‘苹果枣’、‘晋矮3号’果实生长发育时期根系氮、磷、钾、钙、镁含量的变化规律进行研究。结果表明:植株生长初期,根系开始活动,活力不高,植株主要依靠体内贮藏营养物质来提供生长发育所需矿质元素,各枣品种大多数矿质元素的含量有不同程度地减少;植株生长中期通过根系从土壤中吸收大量矿质元素供新器官发育,根系中矿质元素变化较为复杂;在植株生长后期,大多数矿质元素均出现不同程度地增加并趋于稳定,开始为冬季休眠进行营养物质的贮藏。     

基于群体图像识别的生菜鲜重估算方法研究

为提高温室环境最优控制中生菜信息在线反馈精度,通过群体图像识别研究生菜鲜重估算方法;通过生菜群体图像和单株图像,研究群体估算时误差正负相消对整体误差的改善作用,评估生菜遮挡问题对估算精度的影响,并研究能否通过改进深度学习的损失函数以实现对估算精度的进一步提高。结果表明：1)与不存在遮挡问题的单株图像生菜鲜重估算结果相比,基于群体图像裁剪的生菜鲜重估算决定系数(R²)低0.010 8,归一化均方根误差(NRMSE)高2.69%,平均绝对百分误差(MAPE)低2.36%,虽然估算精度略低,但是生菜群体的遮挡问题更能反映生产实际。2)群体估算虽然存在遮挡问题导致裁剪不完整,但根据误差正负相消原理,相比没有遮挡的单株估算结果MAPE仍然低3.49%,因此更适用于生菜产量信息反馈。3)基于更优化MAPE的损失函数平均平方百分误差(MSPE),可以进一步降低群体估算的MAPE至8.46%,满足“软测量”对估算精度的需求。考虑到温室生菜的实际生产情况,群体估算更适合用于温室环境最优控制中生菜产量信息的在线反馈,通过深度学习等方法的优化,可以将生菜产量的估算误差降低至10%以内。     

消费者生鲜水果线上购买迁徙行为及其影响因素

为探索消费者生鲜水果线上购买迁徙行为的影响因素和机制路径,本研究基于PPM理论,构建结构方程模型分析了消费者生鲜水果线上购买迁徙行为及其影响因素,并运用多群组分析不同消费者行为影响因素的差异性。结果表明：1）价格、信息、服务不满意构成正向影响消费者生鲜水果线上购买迁徙行为的推力因素;感知有用性、搜索、消费体验感构成正向影响迁徙行为的拉力因素;程序转移成本构成负向影响迁徙行为的锚定因素。2）锚定因素负向调节推力因素对迁徙行为的作用效果。3）性别、年龄、学历水平三个人口统计特征在不同假设路径中的影响较为显著。其中女性、30岁及以下、学历水平大专以上的群体更愿意在线上渠道购买生鲜水果。结论丰富了生鲜水果领域消费者迁徙行为的研究成果,并为生鲜电商可持续发展提供了启示。