全文获取类型
收费全文 | 1700篇 |
免费 | 66篇 |
国内免费 | 182篇 |
专业分类
林业 | 155篇 |
农学 | 176篇 |
基础科学 | 107篇 |
438篇 | |
综合类 | 719篇 |
农作物 | 74篇 |
水产渔业 | 32篇 |
畜牧兽医 | 177篇 |
园艺 | 24篇 |
植物保护 | 46篇 |
出版年
2024年 | 6篇 |
2023年 | 35篇 |
2022年 | 28篇 |
2021年 | 48篇 |
2020年 | 66篇 |
2019年 | 65篇 |
2018年 | 43篇 |
2017年 | 97篇 |
2016年 | 110篇 |
2015年 | 84篇 |
2014年 | 94篇 |
2013年 | 133篇 |
2012年 | 159篇 |
2011年 | 165篇 |
2010年 | 114篇 |
2009年 | 102篇 |
2008年 | 71篇 |
2007年 | 76篇 |
2006年 | 82篇 |
2005年 | 49篇 |
2004年 | 43篇 |
2003年 | 35篇 |
2002年 | 35篇 |
2001年 | 30篇 |
2000年 | 30篇 |
1999年 | 30篇 |
1998年 | 15篇 |
1997年 | 23篇 |
1996年 | 13篇 |
1995年 | 14篇 |
1994年 | 12篇 |
1993年 | 5篇 |
1992年 | 9篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1984年 | 2篇 |
1981年 | 1篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1977年 | 2篇 |
排序方式: 共有1948条查询结果,搜索用时 640 毫秒
141.
RAPD and SCAR markers linked to the sex expression locus M in asparagus 总被引:13,自引:0,他引:13
Bulk segregant analysis (BSA), random amplified polymorphic DNA (RAPD) and sequence characterized amplified region (SCAR)
methods were used to map molecular markers to the sex locus M of asparagus. Two parents, A19 (male, Mm) and MW25 (female,
mm), and 63 progeny were used for the study. Two DNA bulks, one male and one female, were made by pooling equal amounts of
DNA from 10 randomly selected progeny of each sex type. A total of 760 arbitrary decamer oligonucleotide primers were used
for RAPD analysis. Primer OPC15 produced two RAPD markers, OPC15-98 and OPC15-30, both of which were linked to the M locus
at a distance of 1.6 cM. Subsequently, amplified RAPD fragment OPC15-98 was cloned and sequenced. The sequence was then used
to design flanking 24-mer oligonucleotide SCAR primers SCC15-1 and SCC15-2. Both of these SCAR primers amplified a single
980 bp fragment; the same size as the cloned RAPD fragment. However, the SCAR marker was dominant as was the original OPC15-98
band from which it was derived. These RAPD and SCAR markers could be used for scoring male and female progeny in the mapping
population, but were not found to be applicable to other asparagus germplasm studied.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
142.
S. Ceccarelli 《Euphytica》1987,36(1):265-273
Summary Using the traditional approach (selection for grain yield) it has been found that F3 families derived from F2's selected under unfavourable conditions were more vigorous in the early stages of growth, taller, earlier in heading and with larger yields than F3 families derived from F2's selected under favourable conditions. A high and negative correlation coefficient was found between the drought susceptibility index and grain yield at the driest site, whereas at the wettest site the correlation coefficients were lower and in some cases positive, indicating the existence of traits which are desirable under drought and undesirable under favourable conditions, or vice versa.Expected responses to selection for grain yield using different selection criteria indicated that selection under stress conditions is expected to be more efficient than selection under favourable conditions when dry areas is the target environment.Expected responses to selection for grain yield using different selection criteria indicated that selection under stress conditions is expected to be more efficient than selection under favourable conditions when dry areas is the target environment.Part of this work was supported by Opec Fund for International Development. 相似文献
143.
Summary The effects of bulk breeding on yield and its components in two high-yeilding and two low-yielding bean populations when grown at crop densities are interpreted in terms of gene action and selection. There was considerable additive variation in the crosses, dominance effects were variable but tended to be negative. The results were compared with results obtained at low density by another worker with the same populations, and this showed that interpretations of gene action in hybrid vigour depend on the test environment used. The interpretations were also compared with plant breeding interpretations made on the same material (Hamblin, 1977), and it was found that a single pattern of response to natural selection, in plant breeding terms, may be explained by more than one genetic interpretation and, conversely, a single genetic interpretation may result in different patterns of response in plant breeding terms. The value of composite crosses in plant breeding are discussed and it is concluded that there is a greater chance of producing improved lines from a large number of simple crosses than from a smaller number of composite crosses. 相似文献
144.
The objectives of this study were to optimize RAPD and AFLP techniques in B. catharticus, and to determine the genetic variability of populations and commercial prairie grass cultivars with the aforementioned molecular
markers. Two populations with contrasting morphological characteristics were evaluated from individual and bulked DNA samples
using RAPD markers. Both analyses showed a similar information about inter population variability. Each accession was sampled
by a single leaf bulk of 10 plants. Accession similarities were established with 276 RAPD and 714 AFLP bands using Jaccard
similarity coefficient. The dendrogram of the accessions using RAPD markers showed that they shared high similarity values
(>94%). A similar result was obtained with AFLP markers (similarity values >98%), revealing a narrow genetic basis in the
analyzed accessions. Consequently, molecular characterization of germplasm should be considered in addition to morphological
criteria, to choose the parental genotypes for breeding programs of this forage crop. The AFLP technique was more efficient
to detect DNA polymorphism in our experiments and unique fingerprints were detected for all the accessions. RAPD is a simple
and non expensive technique, suitable to estimate genetic similarity.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
145.
Global wheat (Triticum aestivum L.) production must increase 2% annually until 2020 to meet future demands. Breeding wheat cultivars with increased grain
yield potential, enhanced water-use efficiency, heat tolerance, end-use quality, and durable resistance to important diseases
and pests can contribute to meet at least half of the desired production increases. The remaining half must come through better
agronomic and soil management practices and incentive policies. Analyses of the recent International Yield Trials indicate
that grain yields of the best new entries were usually 10% higher than the local checks globally, as well as within a country
across sites. Variation in yield across sites within a country/region underline the role of genotype × environment (GE) interaction
and provides opportunities to select for stable genotypes, which is not often done. The lack of proper analysis undermines
proper utilization of germplasm with high yield potential and stability in the national wheat breeding programs. Some of the
best performers in irrigated areas were amongst the best in semiarid environments, reinforcing the fact that high yield potential
and drought tolerance can be improved simultaneously. The best performing lines often had genotypic base of widely adapted
genotypes Kauz, Attila, Baviacora, and Pastor, with genetic contributions from other parents including synthetic wheat. We
recommend within country multilocation analysis of trial performance for a crop season to identify lines suiting particular
or different locations within a country. The immediate feedback on GE interaction will also help in breeding lines for countries
having substantial variation across locations and years. 相似文献
146.
Wide mapping of a T-DNA insertion site in oilseed rape using bulk segregant analysis and comparative mapping 总被引:3,自引:0,他引:3
A. Barancer R. Delourme N. Foisset F. Eber P. Barret P. Dupuis M. Renard A. M. Chevre 《Plant Breeding》1997,116(6):553-560
Doubled haploid oilseed rape lines segregating for a transgene inducing herbicide resistance (bar gene) were investigated for the wide mapping of the T-DNA insertion site. Bulk segregant analysis using presence/absence and intensity polymorphisms between the bulks, as well as comparative mapping with a linkage group deriving from another cross, led to the identification of 11 random amplified polymorphic DNA (RAPD) markers tightly or loosely linked to the bar gene. Ten RAPD loci out of 11 were located on the same side of the bar locus, strongly suggesting that the T-DNA integrated in a telomeric or subtelomeric position. The eleventh RAPD marker exhibited a strong segregation distortion, which could be the result of a heteroduplex formation. Comparison of the linkage groups obtained from the two crosses showed different recombination rates between markers, possibly reflecting differences in parental genetic backgrounds. Consequences and potential applications in transgene dispersal safety assessment studies are discussed. 相似文献
147.
Detection of molecular markers linked to the durable adult plant stripe rust resistance gene Yr18 in bread wheat (Triticum aestivum L.) 总被引:2,自引:0,他引:2
Stripe rust of wheat caused by Puccinia striiformis West. f. sp. tritici presents a serious problem for wheat production worldwide, and identification and deployment of resistance sources to it are key objectives for many wheat breeders. Here we report the detection of simple sequence repeat (SSR) markers linked to the durable adult plant resistance of cv. ‘Otane’, which has conferred this resistance since its release in New Zealand in 1984. A double haploid population from a cross between ‘Otane’ and the susceptible cv. Tiritea’ was visually assessed for adult plant infection types (IT) in the glasshouse and field, and for final disease severity in the field against stripe rust pathotype 106E139A+. At least three resistance loci controlled adult plant resistance to stripe rust in this population. Quantitative trait loci (QTL) mapping results revealed that two of these, one on chromosome 7DS corresponds to the durable adult plant resistance gene Yr18 and other on chromosome 5DL were contributed from ‘Otane’; while the remaining one on chromosome 7BL, was contributed from the susceptible ‘Tiritea’. Interval mapping placed the ‘Otane’‐resistant segment near the centromere of chromosome 7DS at a distance of 7 cM from the SSR marker gwm44. The stability of QTL in the two environments is discussed. SSR gwm44 is potentially a candidate marker for identifying the durable resistance gene Yr18 in breeding programmes. 相似文献
148.
江西省是我国13个国家粮食生产基地之一,是保证50年不断为国家提供商品粮的重要省份。为进一步发挥江西省粮食生产潜力,向国家提供更多的商品粮,本文根据多年的研究结果,针对江西省粮食生产的战略地位,建设优质粮仓的资源潜力与关键问题,提出建设优质粮仓的有关建议,供有关方面参考。 相似文献
149.
Rhizophere and bulk soil chemistry were investigated in a Norway spruce stand in SW Sweden. The rhizosphere and bulk soil chemistry in water extracts in control plots (C) and plots repeatedly treated with ammonium sulphate (NS) were compared. Treatment regime was started in 1988. Cylindrical core samples of the LFH-layer and mineral soil layers were collected in 1992 and used for water extract analyses. Samples of soil from LFH-layer and mineral soil layers were taken in 1991 and 1993 for determination of CEC and base saturation. Soil pH and NH4-N, NO3-N and SO4-S, Al, Ca, K and Mg concentrations in water extracts were measured for rhizosphere and bulk soils. The pH-values of bulk and rhizosphere soils in NS plots decreased compared with those in control plots, whereas concentrations of NH4-N, NO3-N, SO4-S, base cations and Al in water extract increased. In both bulk and rhizosphere soils the concentration of NH4-N was much higher than that of NO3-N. A significant difference in the pH and Mg concentration of bulk and rhizosphere soil between the treated and control plots was found only in the 0–10 cm layer. For all layers, there was a significant difference in NH4-N concentrations in the bulk and rhizosphere soil between the NS treatment and control plots. Concentrations of exchangeable base cations and the base saturation level in the LFH-layer decreased in the NS plots. The concentration of extractable SO4-S increased in the NS plots. The NS treatment enhanced the amount of litter in L-layer, owing to increases in needle biomass and litterfall but led to losses of base cations, mainly K and Mg, from LFH-layer. It was concluded that the NS treatment displaced cations from exchangeable sites in the LFH-layer leading to higher concentrations of these elements in both rhizosphere and bulk soil. 相似文献
150.