山羊TNNT3基因可变剪切及其对骨骼肌细胞分化的作用

【目的】快速骨骼肌肌钙蛋白T(fast skeletal troponin T3,TNNT3)作为肌钙蛋白(troponin, Tn)家族成员,调节横纹肌收缩、参与骨骼肌的生长发育并影响家畜肉质性状。通过获得山羊TNNT3基因的可变剪切体,分析山羊TNNT3基因可变剪切的表达模式及其在肌细胞分化中的作用,深入解析TNNT3基因在山羊骨骼肌生长发育过程中的作用机制。【方法】基于NCBI已公布山羊TNNT3基因(NM₀01314210.1)和牛TNNT3基因(XM₀10821200)mRNA序列,使用软件Primer Premier6.0设计引物,以简州大耳羊胚胎期和出生后7个阶段骨骼肌为试验材料,克隆测序获得山羊TNNT3基因的CDS区可变剪切体,利用软件ORF Finder、EditSeq、DNAMAN、ClustalW和MEGA_X10.1.8等对序列进行生物信息学分析;进一步设计实时荧光定量(real-time PCR,RT-qPCR)及半定量引物,研究TNNT3基因剪切体在7个不同组织(背最长肌...     

农业科技论文英语摘要翻译的特点与技巧

随着国际农业交流的日益发展，农业科技论文的数量和质量也在日益提高．英语摘要作为论文中不可忽视的部分，需要运用适当的翻译方法和技巧，以保证翻译的质量．分析了农业科技论文英语摘要翻译的特点和技巧，以及应注意的问题．     

谈李白《清平调》三章诗的英译

李白的《清平调》三章诗问世千多年来,注释家蜂起,各家所见不一,是三章难解亦难译的诗。作者较深入地研究了本诗,于文中提出了“花人合一”之说,并将其译成英文。本文阐述了作者这一观点:译诗者必须具有译诗的素质,诗是随着时间的不断推移、人类交际空间的不断缩小、人类相互了解的不断深化而越来越可译。     

论翻译单位的两个阶段——“句本位”和“篇本位”

翻译单位经历了词层、句层和语篇层三个阶段,此过程体现了翻译研究从静态到动态、从形式到功能的发展趋势。笔者从语言学角度探讨翻译单位“句本位”的局限性及“篇本位”的合理性,指出只有从语篇的层面研究翻译,才能全面、忠实地传递原文信息。     

Investigating translated data of the German soil‐quality assessment using pinpoint field validation

In contrast to modern soil‐profile characterization, alternative soil classifications, such as the German soil‐quality assessment (Bodenschätzung), bear a lower degree of scientific quality. However, despite originally created to determine the tax value of arable land and grassland, its high spatial resolution and complete areal coverage makes soil‐quality assessment a valuable tool. To assess its performance in a mountainous setting soil‐layer data of 60 soil pits, recorded in Bavaria (SE Germany) in the course of the soil‐quality assessment, were translated into German soil‐science terminology using the translation program NIBIS®. With regard to soil type and texture the translation was checked using pinpoint field validation based on soil‐science terminology. 57% of soil types and 61% of texture were correctly translated by NIBIS®. To obtain information about probable parameters that can explain the different results readily available parameters such as elapsed time between soil‐quality assessment and validation, altitude, slope, aspect, horizon thickness, lower edge of horizon, as well as weathering surface and silicate‐weathering rate derived from geological maps were used. Differences in topsoil texture were somewhat related to petrographic parameters, those of the lower subsoil showed a weak dependence to topographic parameters. The NIBIS® translation overrated the silt content to the expense of sand. Clay was the best‐matched texture class. The shift towards silty texture classes was the dominant factor for the differences of texture‐related values of the available water capacity and hydraulic conductivity. Both parameters as derived from the NIBIS® translation on the one and from field validation on the other hand were used to evaluate the water‐retention capacity of individual soil profiles. Despite differing input data the soils' water‐retention capacity was rated identical. Thus, a certain degree of disagreement between the texture data obtained from NIBIS® translation and from field validation is tolerable, if the eventual soil‐function evaluation is based on wide classes of texture or of secondary parameters derived from texture.     

目的论翻译原则下电影《狄仁杰之神都龙王》的字幕翻译探究

狄仁杰在武则天统治时期判决积案、疑案,纠正冤案、错案、假案,是我国历史上以廉洁勤政著称的清官.本文以2013年徐克导演的古装动作片《狄仁杰之神都龙王》为案例,探讨目的论翻译三原则下该影片中的字幕翻译.     

论语境因素在机器翻译中的重要作用

语境在机器翻译中扮演非常重要的角色。本文章结合目前机器翻译的现状，运用计算机辅助英语翻译理论，探讨了语境因素在机器翻译中的重要性，并指出了对源语言进行句子和篇章语境分析有助于计算机进行准确的翻译，从而提高机器翻译质量。     

口蹄疫病毒感染机理的研究进展

介绍了口蹄疫病毒感染过程中的吸附、穿透和脱壳步骤。综述了病毒mRNA的翻译机理以及病毒转录和基因复制的机理。简要描述了病毒粒子的成熟过程，包括病毒RNA正链的装配以及VP0裂解成VP4和VP2的过程。     

重视培养学生的英语汉译技能

分析了英语汉译技能培养的重要性和必要性，并具体提出了一些建议，如帮助学生提高语言分析能力以及传授一些必要的翻译技巧等。