棉花黄萎病菌毒素ELISA检测方法的建立及其应用

以纯化的棉花黄萎病菌毒素(PLPC)免疫新西兰白兔制备了PLPC特异性抗血清,建立了可特异性检测棉花黄萎病菌毒素的A蛋白双抗体夹心ELISA方法。应用建立的ELISA方法测定了病菌培养滤液、人工接种幼苗和大田病株不同组织中的毒素,结果表明:不同产毒能力菌株(VD 8和VD-5)在25℃下振荡培养3d后就能在培养滤液中检测到毒素,这比生物测定要早2～3d。将VD-8菌株的孢子以灌根方式接种泗棉3号幼苗5d后,就能从接种幼苗的茎杆和叶柄中检测到毒素,这比幼苗自然发病显症要早3～5d。在测定的30份大田病株茎杆、叶柄、叶脉样品中,阳性样品率为100%。这些结果表明建立的ELISA方法可用于菌株产毒能力的测定和大田棉花黄萎病的早期诊断。     

Knowledge-based Intelligent Decision-making System for the Safety of Rockets

With the progress of science and technology, the astronautics has obtained great development and researched in many countries, the problem on rocket safety control is important during the rocket launching proceeding, and is also the focus of every space country. At first, the importance of rocket safety control is introduced and the characteristics of the existing system for the safety of rockets are analyzed. After indicating its disadvantages, the design idea, system framework and method for realization of the knowledge-based intelligent decision-making system are proposed to improve certainty and authenticity of safety decision, containing correspond knowledge and adopting the technology of intelligent decision and reasoning on AI.     

Model of Survivable Architecture

Critical information system in Interact is in face of increasingly security problems. More attention has been focused on the survivability of information system, which is the capability of system to continue its mission even in the presence of damage to the system. The authors first introduce the definition of survivability. According to four function properties of survivability, which are Resistance, Recognition, Recovery, and Adaption, the authors design a model of survivable system structure and then explain survivability requirement, survivability strategy in this model. Additionally, they provide possible technical solutions for the survivability of systems, categorized by different aspects of our survivability strategy.     

ANALYSES FOR DEFECTS OF 18G_2ANb STEEL SPHERICAL TANKS OF NATURAL GAS

Calculations and analyses are made with the CVDA, IIW, JWES standards for the strength and welding defects of two spherical tanks of natural gas in CHONG QING. The results of the analyses indecate that though there are some problems with the welding quality in these tanks, they may still be used with the desingned presure. Now the formal working of these two spherical thanks has verified of the validity the calculations and analyses.     

Wavelet Edge Detection Method Based-on Multi-scale Fuzzy Logic

A novel edge detection algorithm is proposed by implementing fuzzy image enhancement at different scales.The implement of this algorithm can reduce the negative influence bring from the non-maximum suppression algorithm which select only one threshold.The success of the approach depends on the selection of a general fuzzy operator(GFO) for the enhancement of contrast of region after smoothing of image at different scales.Consequently,it is more obvious that coarse intensity changes are obtained at a large scale whereas the fine details of intensity changes are obtained at a small scale.A pretty performance is obtained by used this work.     

MACHINE PARAMETER IDENTIFICATION AND FAULT DETECTION FOR THE ALTERNATOR SYSTEM

In this paper, the machine( physical) parameters of the alternator system are on-line estimated on the basis of the derived system continuous-tims model, using Recursive Least-squares Estimation and Inverse Calculation Method proposed by Authors. Therefore, the estimated machine parameters can effectively track the changes of their true values and accomplish the fault detection for the alternator system. This paper gives a new way for machne parameter estimation and fault detection of the alternator systems.     

Study and Practice of Safety Grade Assessment for Large Convention and Exhibition Centre

This paper takes "how to estimate the complex security of Large Convention and Exhibition Centre effectively" as the research goal.Based on analyzing the difficulties,target system,technical constitution,probing into the architecture,safety science,and using for reference the mathematics,operational research,statistics and modern system theory and method,trying to explore establishing an evaluation methodological system,which is provided with integrated characters on subject.Based on these ways,it exerts "quality to quantity" fuzzy analytical hierarchy process(FAHP) and aims at discussing the safety grade assessment of the Chongqing International Conference Exhibition Center focusing on designing theories.The aim is to enhance the integration of design practice and safe consciousness,strengthen the building safety management,and perfect city public security system.In practice,it is also an efficient tool for favoring values of architectural environmental safety.