A Reliable Method for Testing the Sensitivity of Botryotinia fuckeliana to Anilinopyrimidines In Vitro

Cyprodinil is a representative of the new class of broad-spectrum anilinopyrimidine fungicides. The effect of cyprodinil on mycelial growth of Botryotinia fuckeliana on solid agar medium depends on the composition of the medium and on the age of the mycelium to be used for the bioassay. An in-vitro method was developed to study the sensitivity distribution to cyprodinil in two wild-type populations of B. fuckeliana from fruits of strawberry with grey mould. To validate the applicability of the method, sensitivities to cyprodinil, mepanipyrim and pyrimethanil were monitored in populations of B. fuckeliana from grapes with grey mould from different vineyards, including one trial vineyard where reduced performance of cyprodinil had been encountered. The monitoring procedure was based on the inhibition of the mycelial growth on a synthetic medium containing L -asparagine (asp-agar) amended with the active ingredients. The mycelium was grown on asp-agar discs, starting from a spore suspension, for 17 h prior to the beginning of the test. This procedure proved to be efficient. The two wild-type populations from different sampling sites showed similar distributions of the sensitivity to cyprodinil. Some isolates from the trial site with reduced performance of anilinopyrimidines showed reduced sensitivities to cyprodinil, mepanipyrim and pyrimethanil, demonstrating cross-resistance between these anilinopyrimidines.     

湖南柑橘大实蝇成虫生物学研究

对柑橘大实蝇成虫羽化期、田间消长规律及活动习性的研究,结果表明：1、三个观察点成虫羽化始见日至羽化高峰的时间都在5-10天左右,羽化期为16-19天,2011年麻阳的成虫始见日和羽化高峰期比2010年提前了5-8天,2011年长沙的羽化始见日和羽化高峰期都比麻阳稍早2-4天。2、成虫从羽化至基本死亡共持续84天。成虫在田间的始见日和高峰期分别处在5月下旬和6月中下旬,2010年成虫的田间始见日和高峰期都比2011年迟5至7天。3、成虫在交配产卵前,很少飞往柑橘树上,而在橘园及周围杂草或灌木丛中觅食、栖息;当气温超过30℃,成虫飞往橘园周边高大乔木或草丛中避暑,下午16∶00以后飞往柑橘树上产卵。     

高压电晕电场诱变野生草原黑蘑原生质体的初步研究

摘要：为提高草原黑蘑菌丝体生长速度,采用高压电晕电场诱变技术处理草原黑蘑原生质体,通过设置4、5、6、7 kV的电压,分别进行30、60、90、120、150、180、210、240、270 s的处理,统计致死率,结合拮抗试验,初步筛选快速生长的新型突变菌株。结果显示,电压为6、7 kV时致死率均为100%,电压为4、5 kV时可获得原生质体再生菌落,致死率为8.45%～81.69%,其中4 kV处理150 s时致死率最高,为81.69%,是获得突变菌株的最佳致死条件;通过再生菌落的形态观察及拮抗试验,获得14株突变菌株,经生长量及生长速率的测量,发现诱变菌株生长后期4 kV的电压较5 kV更有利于促进菌丝体的生长量,在4 kV和5 kV处理条件下获得了生长速度较CK提高的11株正向突变菌株。高压电晕电场诱变技术 可为草原黑蘑菌种的新品种选育提供参考。     

无公害农产品生产基地环境质量评价研究

以中牟县为例,从大气、农田灌溉水和土壤3个方面,运用单项污染指数法和综合污染指数法,较为系统的评价了其作为无公害农产品生产基地的环境质量现状,并对环境质量监测及评价方法等进行了探讨。     

基于STM32单片机的方形喷洒域装置的设计与试验

针对已有方形喷洒域喷头存在喷灌均匀系数低、方形程度差、转动过程精度不高等问题,设计了一种适用于方形喷洒的新型喷灌装置.该装置主要由控制模块、工作模块和喷头组成;通过滚子与空间凸轮的接触,调节喷头仰角,改变射程,实现方形区域的喷洒;采用单片机控制装置的旋转速度,以提高喷灌均匀度.在工作压力0.20 MPa、流量1.30 ...     

广西某食蟹猴规模化养殖场饮用水和青饲料卫生质量监测

为了对广西某规模化食蟹猴养殖场饮用水和青饲料卫生质量情况进行监测,对饮用水和青饲料进行连续抽样监测,按照《生活饮用水卫生标准》《生活饮用水卫生标准检验方法》和《国家禁用和限用的农药名单》进行检测与评价.结果显示:抽取6份饮用水样检测26项理化指标,有3份不合格,超标率达50.0％,超标指标有浑浊度、肉眼可见物;抽取14...     

基于EDEM小麦收获机清选损失监测试验装置设计

针对小麦损失监测传感器结构复杂、成本较高的问题,对收获机清选排杂口不同物料运动特性进行研究,揭示小麦与秸秆撞击敏感板的作用规律,从清选抛出物冲击敏感板的力学特性出发,设计一种小麦收获机清选损失监测试验装置。通过离散元分析软件EDEM分析小麦籽粒、50mm秸秆、100mm秸秆撞击敏感板产生的作用力,分析接触力变化曲线,证明可通过判断物料撞击敏感板产生的信号进行损失监测。为增强信号采集准确率,采用两片压电传感器串联的方式,增大损失信号。设计损失监测试验台机械结构及控制系统,使监测装置模拟收获机清选排杂过程且可以实时监测信号,有效提高监测效率。最后,通过不同高度物料运动损失监测试验,得出300mm高度下传感器识别较为精准,对小麦籽粒的识别率达到98.4%,整体监测误差小于5%,损失监测试验装置能够达到设计目的和要求。     

Novel soil quality indicators for the evaluation of agricultural management practices: a biological perspective

Developments in soil biology and in methods to characterize soil organic carbon can potentially deliver novel soil quality indicators that can help identify management practices able to sustain soil productivity and environmental resilience. This work aimed at synthesizing results regarding the suitability of a range of soil biological and biochemical properties as novel soil quality indicators for agricultural management. The soil properties, selected through a published literature review, comprised different labile organic carbon fractions [hydrophilic dissolved organic carbon, dissolved organic carbon, permanganate oxidizable carbon (POXC), hot water extractable carbon and particulate organic matter carbon], soil disease suppressiveness measured using a Pythium-Lepidium bioassay, nematode communities characterized by amplicon sequencing and qPCR, and microbial community level physiological profiling measured with MicroResp^TM. Prior studies tested the sensitivity of each of the novel indicators to tillage and organic matter addition in ten European long-term field experiments (LTEs) and assessed their relationships with pre-existing soil quality indicators of soil functioning. Here, the results of these previous studies are brought together and interpreted relative to each other and to the broader body of literature on soil quality assessment. Reduced tillage increased carbon availability, disease suppressiveness, nematode richness and diversity, the stability and maturity of the food web, and microbial activity and functional diversity. Organic matter addition played a weaker role in enhancing soil quality, possibly due to the range of composition of the organic matter inputs used in the LTEs. POXC was the indicator that discriminated best between soil management practices, followed by nematode indices based on functional characteristics. Structural equation modeling shows that POXC has a central role in nutrient retention/supply, carbon sequestration, biodiversity conservation, erosion control and disease regulation/suppression. The novel indicators proposed here have great potential to improve existing soil quality assessment schemes. Their feasibility of application is discussed and needs for future research are outlined.     

基于物联网的日光温室环境监测在线校准

[目的]温室环境条件特别是温度对于作物生长和发育具有十分显著的影响。日光温室调控的主要环境因子之一是温度。然而,自然环境下的光照对温度产生作用,影响空气温度的监测精度。大多温度传感要求将传感器置于避光处,然而实际应用中难以保证。[方法]采用机器学习中的支持向量机算法(SVM),对日光温室内的温度智能监测算法进行了研究,根据光照情况对实时监测的温度数据进行校准。[结果]通过与实验测量的数据进行对比分析,结果表明,所提出的监测方法可以较为准确地实时监测空气温度,从而无需使用隔热材料或者遮阳处理,就可以基于监测的数据更精确地对相应的环境因素进行调节。[结论]基于该方法,可采用常用的工业设备实现温室大棚内实时温度数据的监测,既可以节约设备和人力成本,又可以为温室控制提供准确的数据。     

一种橡胶小蠹虫自制粘捕装置的研制及其田间诱捕参数筛选

橡胶小蠹虫为害重,防治困难。为了探索更加高效的田间防治方法,本研究通过将“牛皮纸+粘虫胶+诱集剂”组配,研究集成了一种橡胶小蠹虫粘捕装置,对其诱捕参数（粘捕装置材质、诱集剂药袋悬挂数量、药液暴露面积以及粘捕装置的间距、悬挂高度）进行了筛选,并与常规诱捕器在重量、成本和诱捕效果方面进行比较。结果表明,在月桂烯、2-甲基-3-丁烯-2-醇、S-(-)-柠檬烯、莰烯、顺式-马鞭草烯醇5种诱集剂的诱集下,自制粘捕带对橡胶小蠹虫的粘捕总数分别为：554、445、341、381、367头;普通粘虫带对橡胶小蠹虫的粘捕总数分别为：260、230、179、202、199头。其中月桂烯、2-甲基-3-丁烯-2-醇、S-(-)-柠檬烯,自制粘捕带的粘捕数量均显著高于普通粘虫带,表现出较好的粘捕效果。采用挂袋法的最佳药袋数为2袋,诱集剂的最佳药液暴露面积为6.6 mm²,粘捕装置的最佳相互间距为50 m,最佳悬挂高度建议在2.5 m。自制粘捕装置在重量、成本以及诱集数量上均优于常规诱捕器,对橡胶小蠹虫防控具有高效成本低、环保可降解、简单易操作等特点。