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21.
水稻低植酸突变基因和外源铁蛋白基因的聚合 总被引:1,自引:2,他引:1
对所获得的2份低植酸突变系和2份铁蛋白(Fer)转基因富铁系稻米进行了无机磷、种子GUS、叶片PCR、稻米矿质元素含量检测和简单的遗传分析,表明这些材料作为低植酸突变种质和Fer富铁种质具有良好的实用价值。对所配杂种F1进行了花药培养,并对愈伤组织诱导、分化、生根和花培苗倍数性、成活率、结实性等性状进行考察,认为低植酸基因和铁蛋白外源基因对花培过程影响不明显。在大量的花培后代中筛选到31份低植酸基因和外源铁蛋白基因双重表达的花培纯系,经检测,该批材料富铁效果极为显著,是难得的低植酸富铁转基因优良新种质。 相似文献
22.
Carlos García Mata Lorenzo Lamattina Raúl Oscar Cassia 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(5):557-562
This work shows that the infection of potato (Solanum tuberosum) detached leaves by the late blight pathogen Phytophthora infestans, was drastically reduced by adding deferoxamine, an exogenous iron chelator. Reactive oxygen species in leaves inoculated with P. infestans were also reduced after adding deferoxamine. A leaf ferritin cDNA fragment was obtained by PCR and used as probe for screening a tuber cDNA library. A cDNA (named StF1) encoding the iron-storing potato ferritin was cloned. StF1 is 915 bp in length and has an open reading frame of 230 amino acids that contains the information for the mature 28 kDa subunit of potato ferritin. StF1 was used as probe in northern blot hybridizations to analyze expression of the ferritin gene. In leaves, ferritin mRNA accumulated in response to pathogen attack. In tubers, ferritin mRNA increased upon treatment with the elicitor eicosapentaenoic acid. These results suggest that iron plays a role in the potato-P. infestans interaction. 相似文献
23.
Filipe Natalio Stefanie Wiese Norman Friedrich Peter Werner Muhammad Nawaz Tahir 《Marine drugs》2014,12(8):4659-4676
Iron, as inorganic ion or as oxide, is widely used by biological systems in a myriad of biological functions (e.g., enzymatic, gene activation and/or regulation). In particular, marine organisms containing silica structures—diatoms and sponges—grow preferentially in the presence of iron. Using primary sponge cell culture from S. domuncula–primmorphs—as an in vitro model to study the Demospongiae spiculogenesis, we found the presence of agglomerates 50 nm in diameter exclusively inside sponge specialized cells called sclerocytes. A clear phase/material separation is observed between the agglomerates and the initial stages of intracellular spicule formation. STEM-HRTEM-EDX analysis of the agglomerates (30–100 nm) showed that they are composed of pseudohexagonal nanoparticles between 5 and 15 nm in size, displaying lattice parameters corresponding to hematite (Fe2O3) and mixed iron oxide phases typically attributed to ferritin. Further analysis, using western blotting, inductively coupled plasma mass spectrometry (ICP-MS), sequence alignment analysis, immunostaining and magnetic resonance imaging (MRI), of mature spicule filaments confirm the presence of ferritin within these organic structures. We suggest that S. domuncula can be classified as a dual biomineralizating organism, i.e., within the same cellular structure two distinct biomineralizing processes can occur as a result of the same cellular/metabolic function, spiculogenesis. 相似文献
24.
25.
Masafumi HASHIMOTO Yasuo NAMBO Takashi KONDO Kiyotaka WATANABE Koichi ORINO 《Journal of Equine Science》2011,22(1):1-7
In mammal circulation, ferritin-binding proteins (FBPs) are thought to be involved in
clearance of circulating ferritin after complex formation with it through
receptor-mediated uptake. However, there is no report on fetal FBP in fetal circulation.
Although iron concentrations of fetal horse plasma were higher than those of adult horse
plasma, plasma ferritin concentrations and ferritin-binding activities were found to be
significantly lower in fetus than in adult. FBPs were purified from fetal or adult horse
plasma on horse spleen ferritin-Sepharose 4B affinity column. Partially affinity-purified
fetal horse plasma FBPs were mainly separated into 65 and 41 kDa bands in addition to
minor bands with higher molecular masses ranged from 102 to 140 kDa on SDS-PAGE under
reducing condition. The adult horse plasma FBPs were separated into 74, 54 and 28 kDa
bands, and the 74 and 54 kDa bands reacted with antibodies specific for horse IgM and IgG
heavy chains, respectively, by immunoblotting analyses. On the other hand, no antibodies
to horse immunoglobulin classes detected any bands in fetal horse plasma FBPs. The
affinity-purified adult and fetal horse plasma FBPs did not contain fibrinogen as a plasma
specific FBP, probably due to its lower affinity to the ligand ferritin. These results
demonstrate the presence of FBPs which are different from adult horse plasma FBPs
including anti-ferritin autoantibodies in fetal plasma. 相似文献
26.
用放射免疫测定法检测126名足月新生儿脐血红细胞铁蛋白含量的结果表明:126例新生儿脐血红细胞铁蛋白为161.4±67.5ag/cell,脐血红细胞铁蛋白含量与血清铁蛋白及血红蛋白含量均呈显著正相关。红细胞铁蛋白含量与体内铁储备密切相关,它不受其他因素的影响,对评价新生儿铁储备的作用优于血清铁蛋白。 相似文献
27.
Two indica rice (Oryza sativa L.) cultivars, viz. ‘Swarna’ and ‘Kalinga III’ were compared for their response to iron (Fe) stress. The cultivars were raised with four Fe levels viz. 0.05, 1, 5, 10 mg L?1 in hydroponic culture. Plant growth, soluble protein, chlorophyll content and phytoferritin of leaves increased significantly with increase in Fe concentration up to 5 mg L?1, but decreased at 10 mg L?1. In contrast, lipid peroxidation, decreased up to 5 mg L?1 then increased at 10 mg L?1. However, at 10 mg L?1 of Fe these parameters were more adversely affected in ‘Swarna’ than ‘Kalinga III’. The later also accumulated relatively more Fe, zinc (Zn), manganese (Mn), and copper (Cu) from the growing medium. Zinc concentrations of the tissue, on the other hand, exhibited the opposite trend. Iron stress may, thus lead to secondary metallic ion stresses and under such situations cultivars like ‘Kalinga III’ will perform better than ‘Swarna’. 相似文献
28.
The surface binding characteristics and permeability properties of the endothelium of secondary lamellae from the gills of
several species of marine teleosts were investigated by introducing cationized ferritin and native ferritin into the microcirculation
under normal environmental conditions. Neither type of ferritin bound to the luminal surface of the gill endothelium. No transcellular
movement of cationized ferritin was detected, either via transendothelial channels or individual vesicles, nor was passage of cationized ferritin through the tight intercellular
junctions of the endothelium observed. Anionic binding sites in the endothelial basement membranes could not be identified.
Binding and transport of ferritin were unaffected by normal changes in environmental temperatures over the range of -2 to
+ 15°C. These findings suggest that the endothelial cell surface of the gills from these fishes is a primary barrier to the
extracapillary movement of blood borne constituents. 相似文献
29.
Yoshiya Kanno Hiromichi Ohtsuka Yasunaga Yoshikawa Kiyotaka Watanabe Koichi Orino 《Animal Science Journal》2013,84(7):556-561
Anti‐ferritin autoantibody is a ferritin‐binding protein commonly found in mammals; it is thought to form an immune complex with ferritin and thereby mediate the rapid clearance of circulating ferritin. The aim of this study is to determine concentrations of ferritin and anti‐ferritin autoantibodies (immunoglobulin (Ig)M, IgG and IgA) in serum and colostrum of Holstein (H) and Japanese Black (JB) cows within 24 h of normal calving. Blood and colostrum samples were collected from cows of various ages (2–11 years) and calving number (1–8 live births). Mean ferritin concentrations were higher in colostrum than in serum for both breeds, and higher colostrum ferritin concentrations were found in H than JB cows. IgA antibodies in serum and colostrum from both breeds had negligible ferritin‐binding activity. For both breeds, IgM and IgG antibodies had higher ferritin‐binding activity in colostrum than in serum. There was a significant correlation between IgM and IgG ferritin‐binding activities in serum and colostrum of H and JB cows. These results suggest that ferritin and IgM and IgG autoantibodies are actively transferred from the blood stream to the colostrum at prepartum or early lactation. 相似文献
30.