食品级过氧化氢对染菌虾仁杀菌效果研究

就新型杀菌技术-过氧化氢杀菌在虾仁加工过程中的应用进行了初步探讨。结果表明:过氧化氢对虾仁的适宜作用条件为:有效过氧化氢浓度1·27g·L-1,作用时间为2min,浸泡液与样品的比例为2:1或浸没样品。过氧化氢溶液受温度影响较大,溶液于低温下放置,随放置时间的延长,有效过氧化氢浓度逐渐降低,操作过程宜在低温条件下进行。过氧化氢溶液处理后的样品宜进行适度的水洗处理,以便去除大量残留在虾仁表面的过氧化氢,同时可以阻止残留过氧化氢对虾仁有机质成分的进一步氧化。     

加快创意农业发展的实践与思考——以浙江德清青虾产业为例

通过分析浙江德清县青虾产业的创意发展历程,得出了德清青虾产业发展的几点启示,并提出了把创意农业提上政府工作日程、加强创意农业文化建设、提高创意农业知名度、丰富农业品牌文化内涵等对策建议。     

垂直流-水平潜流一体化人工湿地对菜地废水的净化效果

广州市番禺区东升农场菜地废水未经处理直接排入水生植物塘,最终流入附近河流,由于菜地废水水质较差,对其造成了严重的污染,严重威胁了附近居民的饮水安全。为了提高菜地出水水质,减轻对河流的污染,通过构建垂直流-水平潜流一体化人工湿地,对菜地废水进行净化处理。湿地经过9个月的运行,结果显示,湿地对COD、NH4+-N和TP的平均去除率分别达56.40%、79.09%和79.79%以上,COD、NH4+-N和TP出水平均浓度分别为9.45、0.47mg.L-1和0.06mg.L-1,均达到《地表水环境质量标准》(GB3838—2002)Ⅱ类水质标准。湿地对TN的平均去除率较低,仅为35.08%,通过补充甘蔗叶作为碳源,湿地对TN的平均去除率显著提高,但随着碳源投加量的增加,去除率逐渐降低。本实验碳源投加量以1.62kg.m-3为宜,TN的平均去除率最高,达80.85%,出水TN平均浓度从3.06mg.L-1降低至0.90mg.L-1。     

明轮驱动虾塘自主导航投饵船设计与可靠性试验

基于明轮驱动的虾塘投饵船能够适应养殖池塘复杂的环境、满足全塘抛撒的要求,可靠性是其进行推广的关键。采用滚塑工艺设计了全封闭投饵船体,利用免油脂润滑不锈钢链轮和明轮作为驱动机构,以避免对水体的污染,螺旋输送饵料装置可满足船载投饵过程中重心位置稳定的要求,通过GPS+电子罗盘的方式实现了自主导航定位和姿态控制需求。根据虾塘投饵和控制性能要求,进行投饵船直线运动和转弯运动模型的构建,采用PID航向、航速运动控制算法进行巡航路径控制,池塘测试平均速度为0.72m/s,直行和转弯最大偏航量分别为0.8m和0.5m。40d的养殖塘现场试验结果表明,自主导航投饵船在复杂路径下运行平稳,可满足虾塘饵料投喂要求,同时对强风、大雨等恶劣环境进行了可靠性测试,发现并解决了相关问题。     

Effects of Rice-Fish Co-culture on Oxygen Consumption in Intensive Aquaculture Pond

Rice-fish co-culture has gained increasing attention to remediate the negative environmental impacts induced by intensive aquaculture. However, the effect of rice-fish co-culture on oxygen depletion has rarely been investigated. We constructed a rice-fish co-culture system in yellow catfish (Pelteobagrus fulvidraco) and freshwater shrimp (Macrobrachium nipponense) ponds using a new high-stalk rice variety, and conducted a field experiment to investigate the effect of rice-fish co-culture on water parameters and oxygen consumption. The results showed that rice-fish co-culture reduced the nutrients (total nitrogen, ammonia-N, total phosphorous and potassium) and the dissolved oxygen content in fish and shrimp ponds. However, they showed similar seasonal change of dissolved oxygen in the water of fish and shrimp ponds. Rice-fish co-culture reduced the total amount of oxygen consumption and optimized the oxygen consumption structure in pond. The respiration rates in water and sediment were significantly reduced by 66.1% and 31.7% in the catfish pond, and 64.4% and 38.7% in the shrimp pond, respectively, by additional rice cultivation. Rice-fish co-culture decreased the proportions of respiration in sediment and water, and increased the proportion of fish respiration. These results suggest that rice-fish co-culture is an efficient way to reduce hypoxia in intensive culture pond.     

热泵干燥北极虾的物理和感观特性研究

对分别在(-2～0)℃和20℃两种温度下热泵干燥北极虾(整虾、去头北极虾、去壳北极虾)的物理和感官特性进行了研究.结果显示,当热泵干燥温度由(-2～0)℃增加到20℃时,干燥虾的收缩率和所需的剪切力增大.干燥温度对干燥虾的色泽影响不显著.所有热泵干燥虾均具有高的复水能力和水分保持能力(WHC).研究结果还显示,虾的处理状态(有壳或无壳、有头或无头、解冻处理)对其物理和感官特性有着显著影响.解冻处理不利于虾色泽的保持并使之收缩增加.在所有的干燥虾样品中,在20℃下干燥的冷冻去头虾(B-2)具有最好的综合表现.与热风干燥相比,利用热泵干燥虾可获得高质量干燥产品.     

基于计算机视觉和XGBoost的虾体活力检测

以南美白对虾为研究对象,提出一种基于计算机视觉和XGBoost的虾体活力检测方法：跟踪对虾应激前后的运动轨迹,提取运动行为特征参数;根据应激性红体现象提取对虾的颜色特征,通过灰度共生矩阵(GLCM)提取虾体应激形成水面波动的纹理特征;运用XGBoost 算法筛选出评价因子,通过加权融合确定评价因子的最佳权重;根据融合后特征对虾体活力强度进行检测。结果表明,提出的方法决定系数为0.905 6,识别准确率为98.61%,较单一颜色、单一纹理以及光流与纹理相结合的方法,识别准确率分别提高6.63%、2.05%和1.61%。     

养殖池塘底泥中恩诺沙星对氮转化的影响

为了研究水产养殖池塘底泥中抗生素残留对氮赋存形态及氮转化功能微生物的影响。选取养殖环境中常见的恩诺沙星（ENR）为目标污染物,设置0、1.0、2.0、5.0 mg/kg 4 个梯度开展模拟试验。结果显示：底泥中NH⁺₄-N和NO^-₃-N含量随试验开展呈下降趋势,添加ENR后,底泥中NO^-₂-N含量增加 7.55%~15.30%;底泥氮转化相关基因中,anammox相对丰度最高,占比60.0%~78.4%。厌氧氨氧化是底泥中氮转化的主要途径,底泥中NH⁺₄-N含量与anammox丰度呈极度负相关;底泥中低含量ENR（1.0 mg/kg）会促进nxrA基因的生长或繁殖,但含量超过2.0 mg/kg时对反硝化基因nirK有明显抑制作用。     

Dietary Photoprotective Compounds Ameliorate UV Tolerance in Shrimp (Pleoticus muelleri) through Induction of Antioxidant Activity

The aim of this study was to determine the effects of ultraviolet radiation (UVR) on bioaccumulation of UV‐absorbing compounds acquired through the diet, in larvae and postlarvae of Pleoticus muelleri, and to assess tissue antioxidant activity, survival, and development. Mysis stage I were exposed to two artificial radiation treatments: M‐PAR (photosynthetically active radiation, range = 400–700 nm) and M‐PAR + UVR (280–700 nm). The experimental larvae received a mixed dietary treatment of Artemia persimilis and the microalga Pavlova lutheri, reared under two radiation regimes: PAR (D‐PAR) and PAR + UVR (D‐PAR + UVR). Shrimp from all treatments reached 8 d postlarval stage (PL8), except those under M‐PAR + UVR treatment fed the D‐PAR‐cultured algae, which had 0% survival. Larvae in M‐PAR + UVR and M‐PAR treatments fed with D‐PAR + UVR diet presented the highest survival rates (70 and 75%, respectively), with 37 and 41% increase in PL size. UV‐absorbing compounds were detected in microalgae and PL subject to PAR + UVR treatments. Antioxidant activity, quantified by measuring the free‐radical 1,1‐diphenyl‐2‐picrylhydrazyl in homogenates of PL8, decayed drastically under radiation treatment M‐PAR + UVR fed with algae of the D‐PAR + UVR treatment. It is concluded that the bioaccumulation of UV‐absorbing compounds and the highest antioxidant activity in PL could improve the biochemical and photophysiological responses of shrimp under UVR stress.     

Performance of Mixed Species and Mono‐specific Algal Diets for Culture of Larval Western School Prawns,Metapenaeus dalli

The effect of three mono‐specific and four combinations of the diatoms Chaetoceros muelleri, Chaetoceros calcitrans, and the chlorophyte Tetraselmis suecica on survival, development, and dry weight of the western school prawn, Metapenaeus dalli, was assessed from protozoea I until mysis I. The development and dry weight of larvae were significantly greater when fed diets comprising C. muelleri and/or T. suecica. A fourth diet, consisting of all three microalgal species also performed just as well. Survival alone was a poor measure of the performance of the various diets. Larvae fed with C. calcitrans, either alone or in a mixed diet with either C. muelleri or T. suecica, had significantly slower development and lower dry weight. Overall performance, assessed using the normalized biomass development index, determined that both mono‐specific and mixed diets containing C. muelleri and T. suecica were among the best for M. dalli larvae. These results for M. dalli are consistent with those found for commercially grown penaeid prawns. This study enhances the limited knowledge on the feeding requirements of metapenaeid prawn larvae. Moreover, the results will help improve hatchery methods for the aquaculture‐based enhancement of M. dalli in the Swan‐Canning Estuary and potentially increase the abundance of this iconic recreational species.