Bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae, changes its host range by duplication and deletion of the small domain in the deduced tail fiber gene

The entire genome of bacteriophage OP₁, lytic for Xanthomonas oryzae pv. oryzae causing bacterial leaf blight of rice, and the partial genomes of related phages were sequenced and analyzed. The OP₁ genome comprises double-stranded, 4785-bp long DNA with 51.1% G + C content. Fifty-nine open reading frames (ORFs) were detected. ORF25 had similarity with the tail fiber gene of phages, whose product is related to host specificity. The ORF25 regions were amplified from four host-range mutants (OP_1h, OP_1hC, OP_1h2, and OP_1h2C) by polymerase chain reaction, and their deduced amino acid sequences were compared. Three mutants (OP_1hC, OP_1h2, OP_1h2C) had duplications of a small domain in the N-terminal portion, although there were slight differences in the position of the duplicated sequences. One mutant OP_1h had substituted amino acids in the duplication region. New mutants isolated in the laboratory (OP_1hC and OP_1h2C from OP₁ and OP_1h2) acquired the ability to lyse strain N5874 belonging to phagovar (lysotype) C. However, they rapidly lost this lytic ability when incubated with other phagovars. This loss was always accompanied by a loss of the characteristic repeats, suggesting that the host range of OP₁-related phages changed mainly through duplication and deletion of a small domain in ORF25. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AP008979, AB214312 to AB214316     

The effects of potato pulp and feeding level of supplements on digestibility, in situ forage degradation and ruminal fermentation in beef steers

Six ruminally cannulated Wagyu (Japanese Black) steers (average initial bodyweight (BW) 387 ± 29 kg) were used in a split‐plot design experiment, comprising a 3 × 3 Latin square design (whole plot) and a randomized block design (subplot). The whole plot treatments were three different feeding levels of supplemental diet, fed at 0.2, 0.4 and 0.6% of BW, on a dry matter (DM) basis. Subplot treatments were two different supplemental diets: a potato pulp silage‐based diet (PPS) and a grain‐based diet (GRAIN). Chopped, medium‐quality cool‐season grass hay (predominately Timothy, Phleum pratense L) was fed daily at 0.7% BW (on a DM basis) as the basal diet. Each period consisted of 21 d, which included 11 d of adaptation to the diets and 10 d of the collection period. Chromium oxide was used as an indigestible marker. In situ forage degradation was measured using the nylon bag technique. The dry matter intake increased (linear; P < 0.01) as the feeding level increased and was not affected by the diet. Digestibility was not affected by any treatments. The GRAIN diet tended to decrease the rate of in situ forage degradation as the feeding level increased, but this trend was not found in the steers fed the PPS diet. Steers fed the GRAIN diet had a lower (P < 0.1) ruminal pH compared with steers fed the PPS diet. Ruminal pH was not significantly affected by feeding level; however, it was numerically higher for steers supplemented at 0.2% per BW than that for the steers supplemented above 0.4% per BW due probably to the higher starch intake. The total volatile fatty acids concentration numerically increased as the feeding level increased and was not affected by the diet. Increasing the feeding level decreased (linear, P < 0.01) the proportion of acetate. Neither diet nor the feeding level had any effects on the proportion of ruminal propionate. The results suggested that, for steers fed the PPS diet, there are not adverse effects on forage digestion in the rumen that occur as the feeding level is increased.     

不同品种肉鸡肌肉的组织学特性研究

采用冰冻切片法、借助TIGER细胞图像分析仪对南海麻黄鸡、江西鸡、黄鸡M系、黄鸡N系、黄鸡K系的胸部肌肉的肌纤维直径和肌纤维密度进行了测定。结果表明:①肌纤维直径由小到大依次为江西鸡(30.1±3.1)μm、南海麻黄鸡(35.3±7.8)μm、黄鸡N系(36.7±1.9)μm、黄鸡K系(37.2±0.7)μm、黄鸡M系(38.1±3.1)μm,江西鸡与黄鸡N系、黄鸡K系之间的差异均达到极显著水平(P<0.01)。②肌纤维密度由小到大依次为黄鸡K系(424.5±39.3)根/mm2、M系(465.5±91.9)根/mm2、N系(471.5±63.0)根/mm2、南海麻黄鸡(568.1±59.7)根/mm2和江西鸡(655.5±182.6)根/mm2。③优质肉鸡的肌纤维直径和密度与肌肉品质之间有密切关系。     

落叶松KP浆碱抽提段强化漂白研究

重点研究了落叶松KP浆CEH三段漂白碱抽提段用H2O2强化的工艺，并对添加螯合剂EDTA的效果进行了研究。     

Effect of Planting Density and Fertilizer Application on Fiber Yield of Ramie （ Boehmeria nivea ）

As the most important cultural practices for ramie (Boehmeria nivea) production,the single effects of plant density and nitrogen (N),phosphorus (P),potassium (K) fertilization on yield are well documen...     

后期热固化处理对竹基纤维复合材料性能的影响

对竹基纤维复合材料进行两种条件(80℃处理8h和50℃处理24 h)的热固化处理后,观测其表观形貌,并进行拉伸、压缩、水平剪切性能以及疲劳性能检测.结果表明:经过热固化处理后,竹基纤维复合材料表面有不连续的微小裂纹产生,其拉伸、压缩、水平剪切性能略有提高,疲劳性能则无显著变化.     

玻璃纤维增强连续塑料复合管道的应用性能

玻璃纤维增强连续塑料复合管是一种多层结构高性能柔性复合管,具有质量轻、抗腐蚀能力强、承压能力强、制作长度长、可弯曲、施工简易、维护方便、节能环保、使用安全系数高的优点,因此整体使用成本较低,有望逐步取代钢制管道,用于石油天然气的开采和输送.建模分析了玻璃纤维增强连续塑料复合管的力学性能,根据不同要求设计了6种玻璃纤维增强连续塑料复合管道,并实施爆破试验,结果表明:实际爆破压力与理论设计压力基本相同,压力范围为5.5～25 MPa,可以满足实际生产需求.     

发酵柑桔渣取代麦麸饲育肥猪试验

柑桔渣经微生物发酵后烘干，以１０％～１５％比例取代麦麸添加到猪日粮中 饲喂育肥猪。饲料适口性良好，对猪的采食和增重均无影响。猪只经屠宰解剖表明，发酵 柑桔渣作猪饲料无任何毒副作用。具有明显的经济效益和社会效益。     

国内外麻类收获机械的现状、问题与对策

回顾了国内外麻类收获机械的研究历程和现状，分析了存在的问题及原因．提出了今后我国麻类收获机械的发展对策。     

云南发展亚麻产业的思路及对策

本文从分析云南亚麻产业发展的可能性和必要性及产业发展现状和存在问题入手，提出产业发展思路及对策，为云南省纤维亚麻的发展提供依据。