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为选择提取岷山红三叶中总异黄酮的最佳方法,本文研究了用回流法、冷浸法和索式提取法提取岷山红三叶中总异黄酮的效果.通过紫外分光光度法测定提取液中的总黄酮含量,结果表明:回流提取法提取效果优于冷浸法和索式提取法.具有安全、简便和提取率高的优点. 相似文献
114.
采用迅速烘干、太阳晒干和缓慢烘干3种处理对云南温带21个牧草品种的鲜草进行干燥处理,分别测定干草的营养成分。结果表明:以干草的粗蛋白含量为指标,干燥方法以迅速烘干为佳,其次是太阳晒干,最差是缓慢烘干;从干草的粗脂肪考虑,其变化无规律,因牧草品种的不同而不同;其余指标变化不大。 相似文献
115.
L.G. Hou H.L. Xiao J.H. Si S.C. Xiao M.X. Zhou Y.G. Yang 《Agricultural Water Management》2010,97(2):351-356
Based on successive observation, fifteen-day evapotranspiration (ETc) of Populus euphratica Oliv forest, in the extreme arid region northwest China, was estimated by application of Bowen ratio-energy balance method (BREB) during the growing season in 2005. During the growing season in 2005, total ETc was 446.96 mm. From the beginning of growing season, the ETc increased gradually, and reached its maximum value of 6.724 mm d−1 in the last fifteen days of June. Hereafter the ETc dropped rapidly, and reached its minimum value of 1.215 mm d−1 at the end of growing season. The variation pattern of crop coefficient (Kc) was similar to that of ETc. From the beginning of growing season, the Kc value increased rapidly, and reached its maximum value of 0.623 in the last fifteen days of June. Afterward, with slowing growth of P. euphratica, the value dropped rapidly to the end of growing season. According to this study, the ETc of P. euphratica forest is affected not only by meteorological factors, but by water content in soil. 相似文献
116.
Evolution of resistance to herbicides in weeds is becoming an increasing problem worldwide. To develop effective strategies for weed control, a thorough knowledge of the basis of resistance is required. Although non‐target‐site‐based resistance is widespread, target site resistance, often caused by a single nucleotide change in the gene encoding the target enzyme, is also a common factor affecting the efficacies of key herbicides. Therefore, fast and relatively simple high‐throughput screening methods to detect target site resistance mutations will represent important tools for monitoring the distribution and evolution of resistant alleles within weed populations. Here, we present a simple and quick method that can be used to simultaneously screen for up to 10 mutations from several target site resistance‐associated codons in a single reaction. As a proof of concept, this SNaPshot multiplex method was successfully applied to the genotyping of nine variable nucleotide positions in the CT domain of the chloroplastic ACCase gene from Lolium multiflorum plants from 54 populations. A total of 10 nucleotide substitutions at seven of these nine positions (namely codons 1781, 1999, 2027, 2041 2078, 2088 and 2096) are known to confer resistance to ACCase‐inhibiting herbicides. This assay has several advantages when compared with other methods currently in use in weed science. It can discriminate between different nucleotide changes at a single locus, as well as screening for SNPs from different target sites by pooling multiple PCR products within a single reaction. The method is scalable, allowing reactions to be carried out in either 96‐ or 384‐well plate formats, thus reducing work time and cost. 相似文献
117.
采用小区试验研究了不同灌溉方式对樱桃番茄结果期根系特性与产量的影响。试验设置了6个灌溉方式处理,即:处理Ⅰ,常规沟灌,种植行和操作行同时灌溉;处理Ⅱ,交替沟灌,种植行和操作行交替灌溉;处理Ⅲ,固定灌种植行;处理Ⅳ,固定灌操作行;处理Ⅴ,前期常规沟灌,结果期交替沟灌;处理Ⅵ,前期交替沟灌,结果期常规沟灌。结果表明,不同灌溉方式之间樱桃番茄根系的特征值差异达到显著水平,处理Ⅵ根体积显著高于其余5种处理,根系伤流量为22.37 mg/min,根系活力高达0.50 mg/(g.h)。灌溉方式对樱桃番茄单果重、产量有显著影响,前期交替沟灌结果期常规沟灌(处理Ⅵ)的单果重与产量均高于其他处理。因此,前期交替沟灌结果期常规沟灌有利于樱桃番茄根系的发育与产量的提高。 相似文献
118.
以新疆生产建设兵团第十二师中型灌区为研究对象,采用首尾法测算2018年和2019年4个样点灌区的灌溉水利用系数,在采用主成分分析法对指标体系降维处理的基础上,用Copula函数构建PCA-Copula评价分析方法,对灌溉水利用系数各影响因素的影响程度和影响规律进行计算分析。结果表明:两年测算数据表明,4个样点灌区灌溉水利用系数都在0.63以上,且最大值达到0.668,分别高于同期全疆、全国平均水平5.05%、10.15%;主成分分析得出,渠道衬砌率(0.944)、滴灌灌溉面积比(0.746)、作物需水量(0.635)、实际灌溉面积(0.734)等都具有超过60%的正贡献率,而葡萄种植比(-0.586)和灌区毛灌溉用水量(-0.645)等具有超过58%的负贡献率。利用PCA-Copula分析评估方法得出,作物种植比例和节水灌溉工程状况对十二师中型灌区灌溉水有效利用系数影响显著(P<0.05),其中葡萄净灌溉定额和灌区毛灌溉用水量对灌溉水有效利用系数的影响极其显著(P<0.01),相关系数分别为0.875、0.742,同时利用Spearman等级相关系数检验法和线型回归来检验PCA-Copula评价法与熵值法的密切程度,检验结果其相关系数分别为 0.87(P<0.05)和0. 52(P<0.001),表明PCA-Copula评价方法适用于研究灌溉水利用系数影响因素。 相似文献
119.
Assessment of inoculation methods for screening black alder resistance to Phytophthora ×alni 下载免费PDF全文
Identification of resistance to Phytophthora ×alni could provide the basis for a management strategy against alder decline in riparian ecosystems in Europe. This study aimed to test methods to evaluate the resistance of riparian alders to the disease, and to screen alder genotypes for resistance. Phytophthora ×alni isolates were compared for their aggressiveness (lesion length on stem) and sporulation capacity (sporangia). While no difference in lesion lengths was found between isolates, sporangia production was dependent on isolate, highlighting the need for careful selection of isolates used for zoospore inoculation methods. Inoculation tests carried out at different periods of the year revealed a seasonal change in susceptibility to the disease, with the period from June to September being the most efficient for inoculation tests. Stem‐wounded inoculations, carried out on excised shoots, were unreliable for evaluating the level of resistance of alder genotypes to P. ×alni infection, with divergent results between two successive years or between two inoculation periods during the same year. In contrast, a method that mimics the natural conditions of infection, based on flooding of rooted cuttings in artificially infected river water, was found promising. Another method, based on the inoculation of foliated terminal shoots with zoospore suspensions, was found to be repeatable and could be used for high‐throughput analyses. Altogether, the results show a continuous resistance response from highly susceptible to moderately resistant genotypes. This suggests that breeding might be a useful strategy to manage alder decline caused by P. ×alni. 相似文献
120.
P. S. Wilson E. O. Ketola P. M. Ahvenniemi M. J. Lehtonen J. P. T. Valkonen 《Plant pathology》2008,57(1):152-161
Stem canker and black scurf are diseases of potato caused by the fungus Rhizoctonia solani . Spatiotemporal experimentation and empirical modelling were applied for the first time to investigate the effect of antagonistic Trichoderma harzianum on the dynamics of soilborne R. solani on individual potato plants. Trichoderma harzianum reduced the severity of symptoms, expressed as 'rhizoctonia stem lesion index' (RSI), during the first 7 days post-inoculation when the inoculum of R. solani was placed at certain distances (30–60 mm) from the host. For example, with inoculum at 40 mm from the host, RSI was 6 and 40 with and without T. harzianum , respectively. At later observation times, the antagonistic effect was overcome. Trichoderma harzianum reduced the severity of black scurf on progeny tubers. Furthermore, the mean number of progeny tubers per potato plant was reduced by the biocontrol treatment (means of 6·5 ± 1·1 and 9·9 ± 2·7 tubers per plant with and without T. harzianum , respectively), as was the proportion of small (0·1–20·0 g) tubers (48% and 66% with and without T. harzianum , respectively). Additionally, there were fewer malformed and green-coloured tubers in pots treated with T. harzianum than in those without T. harzianum . 相似文献