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81.
李玉琴黄新高鹏翔赵小博王慧蒋林树 《动物营养学报》2023,(1):311-322
This experiment was conducted to study the effects of Bacillus amylolyticus on rumen dry matter digestibility, fermentation parameters and metabolites of dairy cows. Experiment 1: six dairy cows with permanent fistula and had the similar parity (2 to 3 fetuses), body weight [(662 ±57) kg], lactation days [(160 ± 22) d] and milk yield [(36.1 ±3.8) kg / d], were randomly divided into 3 groups with 2 cows per group. Using 3 ×3 Latin square design, each group was extra fed 0 (control group), 5 ×109 and 5 ×1010 CFU / d Bacillus amylolyticus, the trial period was 3 periods, 13 days for each period, including 10 days for the pre-trial period and 3 days for the trial period. At the beginning of the trial period, the rumen dry matter digestibility of cows was measured by nylon bag method. Experiment 2: thirty dairy cows with similar parity [(2.5 ±0.3) fetuses], initial body weight [(559.2 ± 7. 4) kg], milk yield [(35. 2 ± 1. 5) kg / d] and lactation days [(99 ± 22) d] were randomly divided into three groups with 10 cows in each group. Cows in the 3 groups were extra fed with 0 (control group), 5 ×109 and 5 ×1010 CFU / d Bacillus amylolyticus, respectively. The pre-trial period was 10 days and the trial period was 42 days. The rumen fluid was collected on the last day of the trial period, and the rumen fermentation parameters and metabolites were determined. The results showed as follows: compared with the control group, 1) supplementary feeding of 5 × 1010 CFU / d Bacillus amylolyticus could significantly improve the rumen dry matter digestibility of dairy cows at 12, 24, 36, 48 and 72 h (P <0.05), supplementary feeding of 5 ×109 CFU / d Bacillus amylolyticus could significantly improve the rumen dry matter digestibility of dairy cows at 36 and 48 h (P <0.05); 2) supplementary feeding of 5 ×109 CFU / d Bacillus amylolyticus could significantly increase the contents of isobutyric acid, butyric acid, isovaleric acid, valeric acid, total volatile fatty acid and microbial protein in the rumen of dairy cows (P < 0. 05), supplementary feeding of 5 × 1010 CFU / d Bacillus amylolyticus could significantly increase the contents of isobutyric acid and isovaleric acid in the rumen of dairy cows and significantly reduce the rumen acetic acid / propionic acid (P < 0. 05), supplementary feeding of 5 ×109 and 5 ×1010 Bacillus amylolyticus had no effects on rumen pH and ammonia nitrogen content (P >0.05); 3) eleven metabolic pathways related to differential metabolites were detected, and ascorbic acid and alginate metabolism were significantly enriched (P < 0. 05) . In conclusion, Bacillus amylolyticus can increase the rumen dry matter digestibility, and improve the rumen fermentation function of dairy cows by affecting the content of rumen metabolites. By comprehensive analysis, supplementary feeding of 5 × 109 CFU / d Bacillus amylolyticus can achieve better effect. © 2023 Authors. All rights reserved. 相似文献
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85.
You lihua Wu Huibi Sun Renbo Wang Daivu 《保鲜与加工》1992,(4):48-53
The basic equations of wall plates of MAN' S dry gas holders are derived in this paper. According to the law of cylindrical bending given by the finite element results .we derive some approximate formula calculating the deflection and stresses of the wall plates from the basic equations. Compared with the known approximate calculating method.the method car. obviously increase the percision. 相似文献
86.
Ye Xiaoming Liu Dongyan 《保鲜与加工》1997,(2):12-20
This paper presents a series of targets which are usefull for engineering.The paper also presents an adaptive weight function according to the fail mechanism of rockmass and a fuzzy aynthetic evaluation medel on rockmass blast ability.The model is applied to evaluate the blast ability of rockmass in an engineering and the result shows that the model is effective. 相似文献
87.
The author introduces a method and experimental procedures that determine dn/dc,molecular weight and its distribution of the hyroxyethyl starch(HES) by means of Gel Permeation Chromatography-Refractive index-Multi-angle laser light scattering detection(GPC-RI-MALLS)techniques.The key experimental technique and results are discussed.The method takes CH3COOH/CH3COONa as mobile phase and solvent.The experiment gets satisfactory results.It can be used as a routine method to determine the hyroxyethyl starch(HES) molecular weight and its distribution. 相似文献
88.
In this paper, a new method of researching; the site dupleface dynamic balance of machine rigid rotator, namely duple test weightmethod is presented. Weight the new method against the existing method,namely single test weight method [1], both the efficiency increasing and theenergy-consumming dicreasing would be forty percent and over, with thebasic points of vibration theory, the formula of calculating weight andposition of the duple face dynamic unbalance of a rotator by means ofapplying the duple test weight is developed in detail. The method in handll-ing distinct bearing form is also discussed. Rightness of the new method isproved out by the living example of the simulant contrast testing carried outn the dynamic balance test machine and the site balance for a rotatorsystem of motor. 相似文献
89.
水稻籽粒中内源多胺及其与籽粒充实和粒重的关系 总被引:22,自引:2,他引:22
以8个水稻品种(含杂交稻组合)为材料,研究了籽粒发育期内源多胺的含量及其与籽粒充实和粒重的关系。结果表明:籽粒中Put(腐胺)、Spd(亚精胺)和Spm(精胺)的含量,强势粒高于弱势糕卫粒充实好的品种(组合)高于籽粒充实差的品种(组合),尤以Spd和Spm更明显;籽闰中Spd和Spm的含量或浓度,以及Spd/Put和Spm/Put的比值与谷粒充实率和千粒軎于极显著正相关在花后3天喷施外源多胺(1m 相似文献
90.
活性多肽提取分离方法研究进展 总被引:3,自引:0,他引:3
综述了近几年来生物活性多肽物质的分离和提纯方法,主要包括色谱、电泳等方法,以及这些方法在肽类研究中的应用现状。 相似文献