甘蓝夜蛾人工饲料的研究

文章报道了以甘蓝叶、白菜叶、灰菜叶、大豆粉、菜豆粉、玉米粉、麦麸为主要原料配制的5种人工饲料对甘蓝夜蛾的饲养效果。结果表明,以甘蓝叶和大豆粉为主要原料配制的配方—人工饲料对甘蓝夜蛾饲养效果最好,甘蓝夜蛾不仅能正常生长发育,而且在产卵量、蛹重、幼虫存活率方面均优于天然饲料,用该人工饲料连续饲养4代甘蓝夜蛾,未发现饲养后代生命力减退现象,表明该人工饲料可用来室内大量连续饲养甘蓝夜蛾。     

扇贝加工废弃物蛋白酶解及其酶解产物分子量分布的研究

对用复合酶酶解扇贝加工废弃物制备水解蛋白的工艺进行了研究,通过正交试验方法确定了用两种复合酶酶解的最适水解条件。结果表明,用枯草杆菌中性蛋白酶和风味酶复合酶酶解的最佳酶解参数为:加酶量比为2∶1,温度为65℃,pH为7.0,时间为5 h,水解液氨基酸态氮含量为50.3%,蛋白质水解度为95.5%。用木瓜蛋白酶和风味酶复合酶酶解的最佳酶解参数为:加酶量比为2∶1,温度为65℃,pH为7.0,时间为5 h,水解液氨基酸态氮含量为43.2%,蛋白质水解度为85.0%。用SephadexG-15葡聚糖凝胶柱层析法对水解产物的分析结果表明,两种混合酶的酶解产物为蛋白肽和游离氨基酸,其相对分子质量均集中分布在585和150左右。     

一株马铃薯干腐病生防菌的鉴定及其抑菌活性研究

镰孢菌引起的马铃薯干腐病是影响马铃薯贮存期间产量与品质的重要病害之一。寻找高效低毒的防治马铃薯干腐病的生防药剂对提高马铃薯的产量与质量具有重要意义。本研究以腐皮镰孢Fusarium solani为病原菌, 测定分离自马铃薯块茎的菌株2-1-9-CJK-2的抑菌活性。该菌株发酵液、无菌发酵液、菌悬液、胞内组分以及挥发性有机物对腐皮镰孢均具有抑菌活性, 抑制率分别为93.37%、61.92%、80.82%、44.01%和49.16%。结合形态学、生理生化特征和多基因系统发育树构建鉴定其为贝莱斯芽胞杆菌Bacillus velezensis。马铃薯切片试验和马铃薯块茎试验表明, 当菌株发酵液浓度为1×109 cfu/mL时, 对病原菌的抑制效果最好。除此之外, 菌株可以分泌蛋白酶、淀粉酶、纤维素酶, 具有溶磷能力和生物膜形成能力, 但不具有溶血性, 并且对供试的其他6种病原真菌有较强的抑菌活性。研究表明贝莱斯芽胞杆菌2-1-9-CJK-2抑菌谱广, 具有生物安全性, 对腐皮镰孢有较强的抑菌活性, 在植物病害防治中具有一定的应用潜力, 可为马铃薯干腐病生防制剂的开发奠定基础。     

茭虾共作对土壤氮磷含量和茭白干物质积累及氮磷钾吸收分配的影响

为探究茭白种养模式的施肥策略,设置茭虾共作（ZC）、茭白单作（Z） 2个处理,研究茭虾共作对土壤氮磷含量、茭白产量和茭白干物质积累及氮磷钾吸收分配的影响。2021-2022年的研究结果显示,茭虾共作模式较茭白单作模式采茭期土壤中全氮、全磷、速效磷的含量分别提高了10.85%~38.89%、5.45%~13.79%、6.19%~61.79%,净茭产量提高了5.10%~19.54%,采茭期茭白茎、叶、净茭的干物质积累量分别增加14.41%~16.58%、10.28%~15.15%、10.57%~21.45%。2021年茭虾共作处理较茭白单作处理在采茭期叶的氮含量显著增加了25.74%,但在孕茭期茎中磷含量显著减少了16.76%;2022年氮肥减量、减量投喂下,茭虾共作处理较茭白单作处理在孕茭期茎的氮含量显著减少了23.75%,在分蘖期叶中磷含量显著减少了12.08%,2 a全生育期同一器官的钾含量在2个处理间差异不显著。对于养分积累,2021年茭虾共作处理在分蘖期叶的氮、磷、钾积累量较茭白单作处理分别减少26.04%、27.27%、30.11%,但在采茭期茎的氮、钾积累量和叶的氮、磷积累量较茭白单作处理分别增加18.80%、38.41%和44.77%、35.71%;2022年在氮肥减量、饲料减量投喂下,茭虾共作处理在采茭期叶的磷积累量和茎的钾积累量较茭白单作处理显著增加30.0%和30.77%。关于养分分配,2种处理的茭白在孕茭期和采茭期氮素的分配均以茎、叶为主,而磷、钾元素的分配均以茎为主,茎分配比例在42%~66%。对于养分转运,2021年2种处理茭白的根、茎、叶在氮、磷转运上大多为表现为“库”器官,而在钾转运上大多表现为“源”器官;2022年氮肥减量、减量投喂下,茭白的根、茎、叶在氮、磷、钾转运量上大多表现为“源”器官。因此,在茭虾共作模式中适当减少肥料、饲料的投入,能促进形成合理的源库关系,有利于茭白产量的形成。     

油菜抗除草剂不育系的抗性效应研究

为探明转育获得的抗除草剂不育系的抗性效应,苗期用不同浓度草甘膦处理抗草甘膦油菜不育系TA6,TA7。结果表明:随着喷药浓度增加,对叶片、茎秆和根的生长表现为不同程度的抑制作用,其中对叶片生长的影响较早且最严重,对茎秆生长的影响较迟,程度相对较轻,对根部生长的抑制作用最小,表现为先抑后扬,但随着时间推移,影响程度逐渐减轻,至喷药60 d后,油菜苗生长基本恢复正常。花期育性调查和套袋自交结果表明,抗性不育系喷施草甘膦除草剂后,育性未受任何影响,TA6,TA7的不育率和不育度均表现正常。     

移栽期气象条件对大棚草莓的影响

通过对8月29日、9月5日、9月12日移栽的大棚草莓生育期跟踪观测,分析草莓各生育期叶面积、器官干物质含量、单棵产量、总产量和气象条件的关系,研究移栽期气象条件对大棚草莓生长发育的影响。第1批移栽的草莓受连阴雨天气影响时间短,生长状况最好,叶面积指数高,各时期干物质积累均多于第2、3批;第2批受连阴雨影响时间较长,植株生长发育一般,产量也一般;而第3批受连阴雨天气影响时间最长,植株生长受严重影响,株高明显矮于前2批,生长状况也较差,前期营养积累少,果实相对较小。移栽期气象条件对大棚草莓后期生长发育和产量及品质影响较大,尤其是连阴雨天气的影响至关重要。     

Effects of post-silking water deficit on the leaf photosynthesis and senescence of waxy maize

Waxy maize is widely cultivated under rainfed conditions and frequently suffers water shortage during the late growth stage. In this study, a pot trial was conducted to examine the effects of post-silking drought on leaf photosynthesis and senescence and its influence on grain yield. Two waxy maize hybrids, Suyunuo 5(SYN5) and Yunuo 7(YN7), were grown under the control and drought(soil moisture content was 70–80% and 50–60%, respectively) conditions after silking in 2016 and 2017. The decrease in yield was 11.1 and 15.4% for YN7 and SYN5, respectively, owing to the decreased grain weight and number. Post-silking dry matter accumulation was reduced by 27.2% in YN7 and 26.3% in SYN5. The contribution rate of pre-silking photoassimilates transferred to grain yield was increased by 15.6% in YN7 and 10.2% in SYN5, respectively. Post-silking drought increased the malondialdehyde content, but decreased the contents of water, soluble protein, chlorophyll, and carotenoid in the leaves. The weakened activities of enzymes involved in photosynthesis(ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase) and antioxidant system(catalase, superoxide dismutase and peroxidase) reduced the photosynthetic rate(P_n) and accelerated leaf senescence. The correlation results indicated that reduced Pn and catalase activity and increased malondialdehyde content under drought conditions induced the decrease of post-silking photoassimilates deposition, ultimately resulted in the grain yield loss.     

干鲜两用辣椒新品种‘安椒12’的选育

‘安椒12’是以‘m 99-6’为母本‘、538’为父本配制而成的杂交1代辣椒新品种。该品种生育期185 d左右,中早熟,始花节位8~10节;果实线形,果长22~25 cm,横径1.8~2.0 cm,平均单果质量21 g,最大单果质量36 g,连续坐果性强,最多可连续不间断坐果136个;早春栽培前期平均667 m^2产量900~1 200 kg,667 m^2总产量3 500~4 500 kg;产量高,抗性强,嫩果绿色,老果红色,适合鲜食和加工。适宜在河南、河北等省春季保护地或春季露地种植。2010年被评为河南省科技进步三等奖;2018年通过了非主要农作物品种登记。     

Cultivation effects on soil texture and fertility in an arid desert region of northwestern China

In arid desert regions of northwestern China, reclamation and subsequent irrigated cultivation have become effective ways to prevent desertification, expand arable croplands, and develop sustainable agricultural production. Improvement in soil texture and fertility is crucial to high soil quality and stable crop yield. However, knowledge on the long-term effects of the conversion of desert lands into arable croplands is very limited. To address this problem, we conducted this study in an arid desert region of northwestern China to understand the changes in soil physical-chemical properties after 0, 2, 5, 10, 17, and 24 years of cultivation. Our results showed that silt and clay contents at the 17-year-old sites increased 17.5 and 152.3 folds, respectively, compared with that at the 0-year-old sites. The soil aggregate size fraction and its stability exhibited an exponential growth trend with increasing cultivation ages, but no significant change was found for the proportion of soil macroaggregates (>5.00 mm) during the 17 years of cultivation. The soil organic carbon (SOC) content at the 24-year-old sites was 6.86 g/kg and increased 8.8 folds compared with that at the 0-year-old sites. The total (or available) nitrogen, phosphorus, and potassium contents showed significant increasing trends and reached higher values after 17 (or 24) years of cultivation. Changes in soil physical-chemical properties successively experienced slow, rapid, and stable development stages, but some key properties (such as soil aggregate stability and SOC) were still too low to meet the sustainable agricultural production. The results of this long-term study indicated that reasonable agricultural management, such as expanding no-tillage land area, returning straw to the fields, applying organic fertilizer, reducing chemical fertilizer application, and carrying out soil testing for formula fertilization, is urgently needed in arid desert regions.     

新疆春小麦育成品种耐热性评价

为筛选出耐热性好的小麦种质材料,通过开花期至成熟期人工模拟高温胁迫环境,以千粒重热感指数为主要评价指标,评价了新疆近30年来审定的春小麦品种的相对耐热性,并分析了高温胁迫对小麦籽粒蛋白质、湿面筋含量的影响。结果表明,与自然生长相比,高温胁迫条件下新疆春小麦育成品种的千粒重、籽粒宽度的变化均达到了极显著水平（P<0.01）,籽粒长度变化不显著(P>0.05),不同品种间耐热性存在很大差异。综合三年千粒重热感指数（HSI）分析,耐热性相对较好的品种有17个,连续三年HSI＜1的品种有11个,其中新春37号、新春2号、新春38号在高温胁迫条下千粒重变化较小,产量较稳定,为强耐热品种;对高温敏感品种有26个,连续三年HSI≥1的品种有13个,其中新春13号、新春18号、新春33号耐热性相对较弱。高温胁迫影响小麦籽粒的品质,其中13.95%的品种蛋白质含量降低,6.98%的品种湿面筋含量降低,其他品种高温胁迫后籽粒蛋白质、湿面筋含量均较自然生长有所提高。