宁夏枸杞叶片组培快繁技术研究

以宁夏枸杞的叶片为外植体,进行愈伤组织诱导、不定芽诱导、分化、试管苗生根、移栽的研究。结果表明:MS+NAA 0.2mg/L+6-BA 1.0mg/L是叶片愈伤组织和不定芽诱导的最佳培养基;MS+6-BA 0.3mg/L+IAA 0.8mg/L是试管苗分化的最佳培养基;1/2MS+IBA0.6mg/L+NAA 0.2mg/L是试管苗生根的最佳培养基。     

根际加温对无土栽培非洲菊冬季产花的影响

 试验结果表明, 非洲菊7 个切花品种冬季无土栽培根际加温可促进其产花。根温为20 ℃时显著提高了切花的产量与质量, 单株产量、花茎长、茎粗、花重较对照分别提高了71. 34 %、19. 14 %、8. 5 %、23. 72 % , 但对花径的增大作用不显著。根温为15 ℃时可显著提高切花产量, 较对照切花增产33. 21 %; 对花茎长、茎粗生长也有促进作用, 但对花径、花重无显著影响。根际加温对不同品种增加产量与提高质量效果不同, 根际加温至20 ℃时, 品种‘日’、‘签证’与‘莫尔’产量较高, 质量优良: 冬季84 d 产量可达9. 90、9. 56 与9. 30 支/株, 花径为10. 21、9. 92 与10. 05 cm , 花茎长为55. 10、53. 61 与54. 82 cm , 茎粗为0. 65、0. 63与0. 61 cm , 花重为21. 52、22. 68 与22. 50 g。     

非洲紫罗兰的组织培养

以非洲紫罗兰叶片切块为外植体,用MS作基本培养基,添加不同浓度的6-BA、NAA等进行诱导分化、增殖、生根培养和过渡炼苗。其中以MS 6-BA0．5—1．0mg／L NAA0．2培养基作诱导增殖培养基较好;生根则采用MS培养基;过渡移栽基质为3：2的腐叶土和珍珠岩,成活率可达90％以上。     

Intensification of redclaw crayfish Cherax quadricarinatus culture: II. Growout in a separate cell system

In the process of exploring ways to intensify crayfish culture, a growout system of individual cages (cells) was designed to determine the effects of gender and cell size on the growth of the red claw crayfish Cherax quadricarinatus. Cells of three different diameters—large (25 cm), medium (20 cm) and small (16 cm)—were used. When crayfish were stocked at a mean weight of approximately 10 g, growth rate of males was significantly higher than that of females. The growth rate of the males in the large cells was 0.31±0.14 g/day, while that of the females was 0.18±0.09 g/day. The size of the cell had significant influence on the weight of males. Male crayfish in the large and medium cells grew better than those in the small cells. When males were stocked at a higher mean weight (about 23 g), their mean weight after 206 days was higher in the large cells (69.28±15.72 g) than in the small cells (58.11±12.66 g), suggesting that the growth of large males was also affected by cell size. Regardless of cell size, male animals of this species grew faster than females under conditions of individual cells. This intensive culture method appears to present a powerful improvement in yields, by as much as two orders of magnitude, in comparison with communal cultures.     

竹荪液体培养适期营养需求的研究

通过单因子试验分析 ,研究了几种碳源、氮源、无机盐对液体培养竹荪菌丝生长的影响和菌丝体生长规律 ,并测定了菌丝和子实体主要营养成分及氨基酸含量。结果表明 ,液体培养 9d ,菌丝生物量最高。菌丝的最适碳源和氮源为蔗糖与黄豆粉 ,无机盐MgSO4 、CaCl2 、ZnSO4 可显著促进菌丝生长。菌丝体与子实体营养成分种类相似 ,但菌丝体蛋白质和氨基酸含量却显著高于子实体     

昌红苹果离体叶片不定芽的诱导及植株再生

 以昌红苹果的组培苗为试材,研究建立叶片的离体再生体系。用30~40 d继代的新梢顶部1~3节新展开的叶片作为外植体,分别接种在分化的培养基上,黑暗处理15 d, 昌红苹果叶片不定芽的最高再生率为100%。在6号培养基上,昌红苹果叶片的再生能力最高; 诱导叶片不定芽适宜的培养基为MS+TDZ 1.0 mg /L+NAA 0.5 mg/L+蔗糖 30 g/L+ 琼脂 5.5 g/L。     

紫薇幼胚培养及植株再生初探

[目的]对紫薇幼胚培养进行尝试性研究,探讨其适宜的生长分化条件。[方法]以紫薇(Lagerstroemia indica)幼胚为外植体,诱导其萌发并建立无菌系。在此基础上探讨了不同激素水平和培养方式对紫薇幼胚培养的影响。[结果]紫薇幼胚去种皮后易于萌发,其萌发率可达100%;启动培养基为MS+BA0.5+NAA0.5+sucrose3.0%+agar0.7%;丛生芽诱导培养基为MS+BA0.5+NAA0.1+sucrose3.0%+agar0.7%+椰乳10%;生根培养基为MS+BA0.5+IBA0.1+sucrose3.0%+agar0.7%+椰乳10%。[结论]该研究可为紫薇的后续优化育种提供技术参考。     

组织培养中青霉素对细菌污染的抑制作用

针对银白杨组织培养细菌污染严重的问题,进行了防治对比试验。结果表明：７～１４℃的温度条件控制细菌污染有一定效果。其污染率为５１％;采用４００或６００万单位／Ｌ的青霉素无菌水溶液处理细菌污染苗,分别浸泡６０ｍｉｎ或４０ｍｉｎ,可有效控制银白杨组织培养细菌污染,其污染率均为０。     

八仙花组织培养繁殖技术

以八仙花的茎段、茎尖、叶片为外植体,在MS培养基中加入不同质量分数的6-苄基腺嘌呤（6-BA）和吲哚丁酸（IBA）进行增殖培养.结果表明：适宜八仙花外植体表面灭菌的方法是利用0.1%氯化汞（HgC l2）灭菌7 m in;不同类型的八仙花外植体在初代培养时的增殖效果不同,茎尖在培养过程中首先伸长生长,然后陆续长出侧芽,表明八仙花的茎尖是比较适合用来进行增殖培养的外植体;继代培养以MS＋6-BA 1.0 mg.L-1＋IBA0.12 mg.L-1为较适宜的增殖培养基;侧芽在1/2 MS＋IBA 0.2 mg.L-1培养基中生根率和单苗根数量均较高,说明1/2 MS＋IBA 0.2 mg.L-1为较好的不定根诱导培养基.     

檫木组培快繁试验

以树龄20 a以上的檫木基部萌条为外植体,对其进行诱导试验,增殖培养及生根培养试验,结果表明,诱导培养MS＋6-BA 0.5～1.0 mg/L＋IBA 0.1 mg/L,诱导率78%,增殖培养MS＋6-BA 0.5～0.8 mg/L＋IBA 0.1 mg/L,增殖倍数4,生根培养1/2 MS＋IAA 0.1～0.2 m...