Characteristic of Inflammatory Airway Disease in Japanese Thoroughbred Racehorses

Inflammatory airway disease (IAD) is a common cause of poor performance, interruption of training and premature retirement in racehorses. It is also reported that up to 80% of horses are affected at some point in the first years of training in UK and Australia. However, no studies with regard to the information on occurrence of IAD in Japanese Thoroughbred racehorses have been reported. To investigate the occurrence and the characteristics of IAD, epidemic research including endoscopic examination of the airway tract and trachea wash was conducted for Thoroughbred racehorses presenting coughs or poor performance which airway tract disease was suspected stalled in training facility managed by Japan Racing Association. Fifty-six out of 76 Thoroughbred racehorses (73.7%) presenting coughing or poor performance were diagnosed as IAD. Mean incidence rate of IAD was 0.3% and it has been confirmed that constant number of IAD exists in Japan. Up to 35.7% of IAD horses showed upper airway abnormalities in some extent. There was a trend for IAD horses to use wood shavings for bedding and fed hay from the ground compared with the control group. Therefore, improvement of stabling environment may aid in preventing IAD. This study demonstrated that Japanese Thoroughbred racehorses are affected by IAD likewise other countries as well as demonstrated the characteristics of IAD which may contribute to the clarification of the pathogenesis of IAD.     

1株野鸭源鸡贫血病病毒的分离鉴定及其编码区基因序列分析

利用MDCC细胞系从东北地区采集的死亡野生鸟类样品中分离获得1株禽类病毒,对该病毒进行特异性PCR、特异性间接免疫荧光法等系统鉴定后,证实该分离株为鸡贫血病病毒(CAV),命名为WDNE110501株.利用PCR方法克隆出其编码区基因片段,测序结果表明,WDNE110501株的编码区全长为1 823 bp,无碱基缺失或插入.并将该基因序列和推导的氨基酸序列与国内外已发表的34个CAV株编码区基因进行同源性和亲缘关系的比对分析,同源性为96.1％～99.8％;氨基酸的同源性为89.8％～99.7％,与国内毒株harbin的差异最小,与国外最近的是美国毒株98D02152.序列比较表明CAV的3个编码基因VP1、VP2和VP3均有一定程度变异,以VP1变异性最大,且在不同毒株间的这3个阅读框的氨基酸序列变异是互不相关的.这是首次在野生鸟类中分离出CAV病毒,提示了我们野生鸟类在鸡传染性贫血病病毒传播和分布中可能起到一定作用.     

饲粮n-6/n-3多不饱和脂肪酸比例对畜禽健康和产品品质的影响

现今人类膳食中n-6多不饱和脂肪酸(PUFA)大多都是过量的,但n-3 PUFA却严重不足.研究表明,n-6/n-3 PUFA比例平衡对畜禽健康和产品品质有着重要意义,n-6/n-3 PUFA比例平衡成为当下最受关注的问题.本文综述了n-6/n-3 PUFA比例平衡的营养生理作用以及对畜禽产品品质的影响及如何改善畜禽产品中的n-6/n-3 PUFA比例.     

蛋鸡淋巴白血病的诊断

2011年某蛋鸡场部分病鸡内脏发生增生性肿瘤性疾病,经流行病学调查、临床诊断及实验室检测,确诊为鸡J亚型淋巴白血病。文章就该病例的诊断过程做了详细阐述,并对禽常见传染性肿瘤病的鉴别、白血病的预防和控制进行了归纳总结。     

凉山草坪病虫害及其防治途径初探

本文在调查研究凉山草坪病虫害的基础上,对草坪的病虫害防治进行了初步探讨和研究,并提出了解决途径.     

锡林郭勒盟草原莱姆病疫源地调查

本项研究于2000年5月—2001年5月从内蒙古锡林郭勒盟地区，不同草原景现带牛、羊体表和草地采集草原血蝉1800只，用直接荧光抗体法检查蜱带菌情况，带菌率为7.5%(6/80)。晴视野显微镜检查蜱带菌情况，带菌率为2%（2/100）；用间接荧光法进行血清流行病学调查，结果证实，调查点的牛和野鼠均存在莱姆病螺旋体感染，其感染率分别为10%和20%，都高于当地人群平均感染率（7.09%)，上述研究证实内蒙古锡林郭勒盟地区人畜及野生动物均有莱姆病螺旋体感染。     

扎布耶盐山羊大肠杆菌分离鉴定及药敏试验

为掌握西藏自治区仲巴县帕江乡扎布耶盐山羊的发病情况,采集发病羊的粪便、血液、肝脏、脾脏、胸腔、腹腔和心包腔内蓄积的浆液性纤维性渗出物等进行病原菌分离鉴定及药敏试验。经病原菌分离鉴定,该地区扎布耶盐山羊羔羊感染的病原菌为大肠杆菌,检出率为97.14%(34/35);药敏试验结果表明,该研究分离得到的大肠杆菌对头孢唑林、青霉素、阿莫西林、头孢他啶、氟苯尼考、头孢拉啶6种药物高度敏感。该调查研究为仲巴县帕江乡扎布耶盐山羊大肠杆菌病的防治提供了诊断方法和用药方案。     

甘肃省肃南县草地治蝗灭鼠工作经验

总结了肃南县１９５５年以来草原治蝗灭鼠为单一人工药物防治发展到生物新技术与新药物相结合的综合治理经验,提出了今后科技力量,开展多渠道,多学科,多部门协作防治的发展方向。     

猪繁殖与呼吸综合征病毒实验感染SPF猪外周血单核细胞和肺泡巨噬细胞早期凋亡细胞的观察

采用Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ／ＰＩ双染色法,用流式细胞仪检测了猪繁殖与呼吸综合征病毒（ＰＲＲＳＶ）实验感染ＳＰＦ猪不同时期外周血单核细胞和肺泡巨噬细胞感染Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群（早期凋亡细胞群）。结果显示,ＰＲＲＳＶ感染猪外周血单核细胞和肺泡巨噬细胞Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群的表达率均明显高于正常对照猪,感染后２４ｈ表达率达最高值。