张掖市牛产业发展现状及几点建议

牛产业做为张掖支柱产业,近年来,为助力脱贫攻坚和乡村振兴发挥了非常重要的作用,但随着产业的发展,暴露出很多短板弱项问题,对此,必须结合出现的问题,提出针对性的意见建议,保障以后产业的高质量发展,为进一步产业增效、农牧民增收发挥积极的贡献。     

牦牛MYL3基因的克隆及组织表达分析

【目的】研究牦牛MYL3基因的结构和表达特征,探究其生物学功能及影响牦牛肌肉生长发育的机制。【方法】以美仁牦牛cDNA为模板,PCR扩增MYL3基因编码区序列(CDS),利用MEGA11软件构建系统进化树,利用生物信息学软件对其编码蛋白进行分析。通过实时荧光定量PCR(RT-qPCR)检测MYL3基因在心脏、肝脏、脾脏、肺脏（参照）、睾丸、肌肉和脂肪组织中的相对表达量。【结果】牦牛MYL3基因CDS区长600 bp,共编码199个氨基酸,存在1个碱基突变位点,位于第165位(A>T)。系统进化分析结果显示,牦牛与普通牛的亲缘关系最近,与单峰驼的亲缘关系最远。生物信息学分析结果显示,牦牛MYL3蛋白为不稳定的亲水性蛋白,无信号肽,不存在跨膜结构,主要存在于细胞质内,有8个磷酸化位点和2个N 糖基化位点,蛋白的二级结构以α-螺旋为主,主要与调控肌肉生长发育的相关蛋白相互作用。RT-qPCR结果表明,MYL3基因在心脏中的表达量最高,极显著高于其他组织;肌肉中次之,与除心脏外的其他组织存在极显著差异。【结论】MYL3基因可能与牦牛心脏发育有关,对肌肉的生长发育和肉质有一定影响。     

农产品冷链物流高质量发展水平评价及障碍度分析

推动冷链物流高质量发展,对减少农产品产后损失、扩大消费品优质供给、保障食品安全有重要作用。基于我国2012-2021年统计数据,构建了包括经济基础、物流发展、冷链运营、开放创新、绿色协调5个一级指标及21个二级指标的农产品冷链物流高质量发展评价指标体系,利用熵值法确定各指标的权重,综合评价指数确定得分水平,借助障碍度模型分析发展障碍因子。结果显示：我国农产品冷链物流高质量发展水平总体呈上升趋势且增速较快,到2021年水平值达到0.85,是初始状态的近13倍,其中开放创新水平的贡献度最高;阻碍高质量发展的障碍因素在2017年之前为开放创新水平,之后则是绿色协调水平。为进一步提高我国农产品冷链物流高质量发展水平,需要充分利用国内外两个市场、促进产业协同、发展绿色低碳物流。     

牛角瓜果实发育规律及纤维产量

为引进优质种质资源打下基础,阐明牛角瓜果实发育的规律,制定栽培管理措施,以实现品质的调控和果实产量的稳定。因此本试验通过对巴基斯坦瓜达尔港的牛角瓜果实外部特征、内部纤维发育过程的观察。确定了牛角瓜果实外部大小与内部纤维长度呈正相关,即果实越大,纤维长度越长。在座果后第1天至第6天为缓慢增长期,此时应采取保果措施;第7天至第19天为果实增长和纤维快速增长期,此时应给予足够的养分;在第20天至第30天为缓慢增长期,此时应侧重于病虫害的防治。     

东台市工厂化育苗产业的发展现状及建议

东台地区顺应市场农业现代化发展需求,支持和推进瓜果蔬菜育苗产业化,已形成一定规模和技术体系,但也存在企业运营水平差异、缺乏专业技术培训、产业经营淡旺不均等问题;因此,要探讨加快工厂化育苗产业健康发展的途径和思路,尤其要构建质量与风险管控机制、创建个性化品牌、提升售后服务水平,旨在促进瓜果蔬菜育苗产业化经营的标准化、现代化。     

ISO质量管理体系在湖南水文的建立

陈述了ISO9000质量管理体系在湖南省水文水资源勘测局的建立过程及带来的管理观念转变,分析了质量管理体系对本单位的利与弊。     

Penetration, Development and Emigration of Juveniles of the Nematode Meloidogyne arenaria in Myrobalan Plum (Prunus cerasifera) Clones Bearing the Ma Resistance Genes

Penetration, development and emigration of M. arenaria in the roots of three Myrobalan plum (Prunus cerasifera) clones genetically characterized for their resistance to root-knot nematodes (RKN) were studied during the 10 (penetration) and 15 (emigration) days following the date of inoculation (D) of 2500 juveniles (J2s) per plant into the soil. Miniaturized tests were conducted on the two resistant clones P.2175 (Ma1 gene) and P.1079 (Ma2 gene) and the susceptible clone P.2032 (recessive for both genes), obtained from micropropagated plantlets and grown in mini-containers under controlled conditions at 25°C in a growth chamber. For penetration and development studies, nematodes in the roots were recovered by the acid fuchsin-lactophenol staining technique. Equivalent numbers of J2s were recovered in all the clones at D+1 and D+2. Subsequently, the numbers increased rapidly in P.2032 and were significantly different from those in P.1079 and P.2175 that remained at a low level. No swollen larvae were observed in the resistant clones. In P.2032, the first swollen larvae were observed at D+4, the first females were observed at D+12, whereas the first females with attached egg sacs and the first new-generation J2s were obtained between D+21 and D+28. Our data suggest that the resistance phenomenon does not act on the very early nematode penetration but acts later by preventing feeding-site induction and development into the third-stage. For emigration studies, plants in which J2s had been allowed to penetrate for two days (from D to D+2) were washed free of soil, repotted and then, after various periods of growth, soil-free roots were placed under a mistifier to evaluate the numbers of emigrating individuals. Emigration of J2s from the roots occured mainly from D+2 to D+4 in all the genotypes and was very limited from D+4 to D+10. There was no significant differences in the number of emigrated juveniles between the resistant and susceptible clones, indicating that emigration cannot explain the difference in the numbers of nematodes recovered in the roots.     

天山北坡经济带人口分布与经济发展研究

人口分布是影响经济发展的重要因素。人口分布与经济分布的最基本属性是不均衡性,人口与经济的空间集中化是区域发展的内在规律。本文从人口与经济发展的关系出发,通过对天山北坡经济带人口数量、人口密度、人口分布等因素与经济发展相关关系及动态演进的多方面分析,为天山北坡经济带进一步实施人口与经济空间集中化战略,建立起人口、经济与环境协调发展的区域格局提供一些理论依据与对策。     

包头市城市环境与经济协调发展分析与评价

运用层次分析法从综合环境效益和综合经济效益两个方面构建指标体系,建立研究模型,定性与定量相结合,从包头市城市环境与经济发展的协调度方面,确定包头市环境与经济协调发展的类型,对2001年-2005年以来包头市的城市环境与经济协调发展状况进行了分析与评价。     

淮安红椒产业现状及发展对策

结合淮安红椒产业发展现状、特色以及存在的问题,提出成立淮安红椒产业发展办公室、培植壮大龙头企业、建设规模的淮安红椒标准园、成立淮安红椒研发中心、发展淮安红椒文化产业等相应对策.