水牛犊牛血浆外泌体的分离与鉴定

研究旨在从水牛犊牛血浆中分离外泌体,并对分离的外泌体进行分子生物学特征分析和鉴定。采用差速离心法分离3头水牛犊牛的血浆外泌体,并在透射电子显微镜下观察其形态,使用纳米粒度及Zeta电位仪对提取的外泌体进行粒径大小分析,应用Western blotting方法检测外泌体标记蛋白钙联蛋白（Calnexin）、肿瘤易感基因101（TSG101）、CD9、CD81在3头水牛犊牛血浆外泌体中的表达情况。结果显示,从水牛犊牛血浆中分离的外泌体形态多为圆形和椭圆形,直径在30~150 nm;Western blotting检测结果表明,在水牛血浆外泌体表面,特异性蛋白Calnexin、TSG101和CD81阳性表达,CD9不表达。本研究从形态学和分子生物学特征等方面证实研究获得的提取物为外泌体,由此说明,差速离心法可以成功分离提取水牛犊牛血浆中的外泌体,为水牛外泌体的后续研究提供了重要技术基础和参考。     

鸡呼吸道疾病鉴别诊断与防治

呼吸道疾病是目前威胁鸡养殖产业健康发展的一类重大动物疫病,呼吸道疾病的病原种类多种多样,且造成的临床症状大致相同,诊断难度较大,如果没有进行妥善有效的鉴别、诊断,易造成整体的治疗方案缺乏针对性,难以在短时间内控制病情,错过最佳的防控时期。鸡呼吸道疾病常常混合感染并发感染,2种以上的病原相互叠加表现的临床症状复杂,诊断难度更大。该文主要论述鸡呼吸道疾病的鉴别诊断和防治措施。     

微分响应在降雨误差修正中的应用

误差修正是实时洪水预报研究和应用中的重要内容,从修正模型降雨输入角度出发,提出了一种基于微分响应的降雨误差修正方法,并通过实例论证了该方法修正降雨量的适用性。该方法通过计算降雨所对应模型的微分响应来修正降雨,从而修正出口断面流量过程。将该修正方法结合新安江模型,对闽江建阳流域的19场历史洪水进行了有效性检验,结果表明:此方法对洪量、洪峰的修正效果明显,且能够显著提高洪水预报的合格率,修正后合格率由63.2%提高到94.7%。     

Utilization of digital differential display to identify differentially expressed genes related to rumen development

This study aimed to identify the genes associated with the development of the rumen epithelium by screening for candidate genes by digital differential display (DDD) in silico. Using DDD in NCBI's UniGene database, expressed sequence tag (EST)‐based gene expression profiles were analyzed in rumen, reticulum, omasum, abomasum and other tissues in cattle. One hundred and ten candidate genes with high expression in the rumen were derived from a library of all tissues. The expression levels of 11 genes in all candidate genes were analyzed in the rumen, reticulum, omasum and abomasum of nine Japanese Black male calves (5‐week‐old pre‐weaning: n = 3; 15‐week‐old weaned calves: n = 6). Among the 11 genes, only 3‐hydroxy‐3‐methylglutaryl‐CoA synthase 2 (HMGCS2), aldo‐keto reductase family 1, member C1‐like (AKR1C1), and fatty acid binding protein 3 (FABP3) showed significant changes in the levels of gene expression in the rumen between the pre‐ and post‐weaning of calves. These results indicate that DDD analysis in silico can be useful for screening candidate genes related to rumen development, and that the changes in expression levels of three genes in the rumen may have been caused by weaning, aging or both. © 2015 Japanese Society of Animal Science     

Flexure-torsion Analysis of Variable-curvature-box Girder Considering Non-coincidence of Torsion Center and Gravity Center

In order to consider the effect of non-coincidence of torsion center and gravity center,the rectangular variable-curvature-box girder with vertical symmetrical axis is studied. Considering the influence of shear deflection and non-coincidence of torsion center and gravity center, the elastic equilibrium formulas are established. Based on deformation equations of the girder and the relations of inner forces and deformations,the flexure-torsion differential equations are developed. The equilibrium differential equations and Galerkin methods formulas for clothoid curved box girder are presented.     

Case- carburization Numerical Modeling and Craft Optimization on Steel Parts of Various Shapes

According to the diffusion equation of carburizing process and its boundary conditionand initial condition, various shapes steel parts are modeled under various case carburize craft parameters, concentration distribution curves have been obtained. Various craft parameters are compared, and carburizing temperature has more influence on carbon concentration distribution. Various shapes steel parts' carbon concentration distribution curves have some difference. According to the carbon concentration distribution curve, craft parameters can be adjusted in order to obtain the best carburizing craft.     

The Multiple Solutions for a Class of Quasilinear Elliptic Equations

A three critical point theorem is proved. As its applications, Dirichlet problem for a class of quasilinear elliptic equations cf second order is considered.     

THE BASIC PROPERTIES OF CONDITIONAL EQUATION WITH ACCUMULATIVE STATE

Several aspects of the conditional equation derived from the accumulative equation are discussed in detail. The results indicate that the conditional equation not only is well consistent with the principles of electronegativity and absolute hardness equalization, but also satisfies the bonding of a full -prerequisite condition, e. g. two like electronic charge species repel and unlike attract each other'. In addition these principles are in the accumulative state. Mathematically, some other aspects of the conditional equation are worth studying based on the generalization of this equation.