胶陀螺人工培养特性的研究

本文论述了胶陀螺在不同培养基上，以及温度、光照、湿度等不同条件下的人工培养状况，并可以通过液体繁殖、扩大，完全可以省去人工栽培生产过程。     

华南忍冬组培苗与扦插苗植株绿原酸比较分析

【目的】研究华南忍冬组培苗植株的主要药用有效成分含量,为其组培苗开发利用及规模化生产提供参考。【方法】采用超声波法提取、紫外分光光度法对华南忍冬组培苗1年生植株和扦插苗1.5年生植株的不同部位绿原酸含量进行测定。【结果】组培苗植株各发育时期花的绿原酸含量为2.06%~3.89%,以幼蕾期的含量最高,金花期最低,均符合国家规定的药用金银花商品的标准要求。组培苗1年生植株、扦插苗1.5年生植株各部位的绿原酸含量均表现为花＞叶＞枝条,各部位之间含量差异较明显。扦插苗1.5年生植株花蕾、叶片和枝条的绿原酸含量略高于组培苗1年生植株,但两者在白蕾期花蕾的绿原酸含量无显著差异,而叶片和枝条的绿原酸含量差异达显著或极显著水平。【结论】华南忍冬组培苗花蕾的绿原酸含量虽低但与扦插苗差异不显著,根据组培苗在苗木移栽、植株营养生长、花蕾产量等方面的生长优势,可在生产中推广种植山银花组培苗。     

Role of Cl- in regulatory volume decrease of nasopharyngeal carcinoma cells

AIM:To clarify the role of Cl^- in regulatory volume decrease (RVD) of nasopharyngeal carcinoma cells (CNE-2Z).METHODS:Analysis of living cell images was used to detect the volume changes following exposure to hypotonic solution. Iron replacement and block of iron channels were also applied in the present study. RESULTS:Extracelluar hypotonic treatment made the cells swell and induced RVD. The RVD was correlated positively to the swelling in the range of 160-230 mOsmol/L. Substitution of gluconate for Cl^- in perfusing solutions markedly increased RVD. Depletion of cellular Cl^- abolished, and chloride channel blockers inhibited RVD. CONCLUSION:Cl^- is the key iron to establish the RVD in CNE-2Z cells. Activation of Cl^- channels and Cl^- efflux are the major mechanisms of RVD.     

闽东主要贝类养殖区养殖贝类卫生质量状况调查

2004-2006年对福建闽东主要贝类养殖区共计40批次经济贝类开展卫生质量状况调查。抽查品种包括太平洋牡蛎、褶牡蛎及缢蛏等,调查项目包括重金属、石油烃、微生物及贝毒等。调查结果表明,除部分贝类产品受到粪大肠菌群及石油烃污染外,其他调查指标结果较为良好。     

养殖牡蛎的敌害

论述了养殖牡蛎面临的敌害问题,所涉及的敌害包括绿蛎病、瘤、敌害生物、赤潮、环境压力和综合性因素的恶化等,相信中国的相关研究将走向深广。     

Effects of lipopolysaccharide and interleukin 1 receptor antagonist on proliferation and nitric oxide synthesis of mesangial cells

AIM: To investigate the effects of lipopolysaccharide (LPS) and interleukin-1 receptor antagonist (IL-1ra) on mesangial cells proliferation and nitric oxide synthesis. METHODS: Glomerular mesangial cells from SD rats were cultured. The first and second passages of cultured cells were used for the experiment. LPS and LPS plus IL-1ra were added in cell cultures, respectively. By using chemical method the nitrite in supernatants was measured, [³H]-TdR incorporation was determined to evaluate the GMC proliferation. Northern and slot hybridizations were performed to detect the expression of iNOS mRNA. RESULTS: There were expression of iNOS mRNA, more production of nitrite (0.64±0.25 vs 0.12±0.06 nmol/10⁴ cell) in supernatants and GMC proliferation (3735±1177.9 vs 1785±280.6) in LPS group compared to the control. While compared with LPS group, in LPS+IL-1ra GMC group, expression of iNOS mRNA decreased by 40%, nitrite increased (3.28±0.33nmol/10⁴ cell), proliferation of GMC decreased (818±77.27). CONCLUSION: LPS could activate the GMC to express iNOS mRNA and produce more nitrite. IL-1ra could partially inhibit the effects of LPS on the expression of iNOS mRNA in GMC, but not nitrite. There is no synchronous correlation between NO production and GMC proliferation.     

养殖水环境中亚硝酸盐对鱼类的危害及防治的研究

调查和监测60口优质鱼类养殖池塘中水质理化因子和生物因子共31项,统计结果,鱼类患病的池塘亚硝酸盐含量比未患病的池塘高,其含量均值分别为0．176mg／1和0．095mg／l,以彭泽鲫鱼为实验材料,测定亚硝酸盐对血红蛋白的毒性影响,结果表明,鲫鱼血液中的MHB随NO2－N浓度的增加呈指数递增关系,据实验结果统计分析,1．8mg／l和0．8mg／l分别为48h和96h毒性影响的临界值。养殖水体增加足量溶氧,适量的氧化剂,氯离子和硝化细菌,可降低亚硝酸盐的含量。     

海洋养殖生物体中石油烃含量分布及变化情况研究

根据2005～2007年三年来福建沿海养殖生物体中的石油烃(TPH)检测数据,对鱼类、贝类、甲壳类删含量的分布和变化进行研究.海洋养殖生物体Ⅱ'H含量范围在(1.00～179.71)×10-6湿重,总平均值为10.60×10-6湿重.TPH总体平均含量年份变化:2006年>2007年>2005年;海洋养殖生物体部分类别差异:贝类>甲壳类>鱼类;贝类中缢蛏含量最高,鲍含量最低;贝类体中胃含物>肌肉组织>体液.平均TPH污染指数<1,总体属轻度污染.