阿月浑子总酚含量与褐变关系研究

对阿月浑子不同器官、同一器官不同季节以及组培苗不同继代时期,植物体内总酚含量变化的研究。结果表明,阿月浑子富含多酚物质,叶片总酚含量最高,不适合组培试验;茎段含量较少,较适宜组培且春季取材最佳。组培苗随着继代次数的增加酚类物质总量也有所增加但是幅度不大。通过对褐化抑制剂的筛选,确定适合阿月浑子组培实验的抑制剂为PVP,其剂量以1.3~1.5 g/L较好。     

无机盐质量浓度及激素配比对四倍体刺槐组织培养苗生根的影响

在四倍体刺槐试管苗的生根培养阶段,诱导愈伤组织形成根原基,主要取决于6-BA和NAA的质量浓度配比。试验结果表明,在生根培养过程中,单加NAA0．3mg／L,可使组织培养苗发根早,根系发达,须根多,最终能形成完整的健壮植株;当培养基采用低无机盐质量浓度的Ms培养基即l／2MS时,试管苗发根早,且根多而粗壮。     

Changes in myoglobin content and proximate compositions of the dorsal ordinary muscles of full-cycle cultured Pacific bluefin tuna Thunnus orientalis (Temminck et Schlegel) with body size

We investigated the changes in myoglobin (Mb) content and proximate compositions of the cephalad parts of the dorsal ordinary muscles (Ce‐DOM) of full‐cycle cultured (FC) Pacific bluefin tuna [body length: 42.6–115.4 cm, body weight (BW): 1.7–38.4 kg, killed from July 2004 to October 2005]. The Mb content of Ce‐DOM increased (P<0.01) until a BW of 7.0 kg was reached. However, the Mb content of Ce‐DOM had a wide variety above a BW of 7.0 kg. On the other hand, the moisture, protein and ash contents of Ce‐DOM of FC tuna decreased (P<0.05 and 0.01) with an increase in BW. However, the lipid content of Ce‐DOM increased gradually above about a BW of 20.0 kg. Furthermore, the Mb content in protein of the Ce‐DOM of FC tuna increased with an increase in BW. These results indicated that the increase in Mb content of Ce‐DOM of FC tuna was not related to changes in proximate composition between a BW of 1.7 and 38.4 kg.     

大黄鱼一年两次性成熟发育特征的研究

选择春季产过卵的2^ 龄大黄鱼Pseudosciaena crocea(Richardson)在网箱中进行隔离培育，秋季从中挑选当年再次性成熟的亲鱼进行人工催产，比较春、秋两季的人工繁殖与仔、稚鱼培育效果，并定期观察卵巢及卵细胞的发育情况。实验表明，春季产过卵的同一批大黄鱼雌鱼，有72．1％的个体当年秋季可再次成熟，有58．8％的个体可以再次产卵，证实养殖大黄鱼具有一年“两熟”的性腺发育特征。     

规格与盐度对凡纳滨对虾肌肉营养成分的影响

分析测定了海水池塘养殖不同规格(8.3、12.5、16.7g.尾-1)凡纳滨对虾(Litopenaeus vannamei)及土池淡水养殖规格12.5g.尾-1凡纳滨对虾肌肉中水分、蛋白质、脂肪、灰分、脂肪酸和氨基酸组成,并进行品质评价。结果显示,海水养殖对虾肌肉的水分、脂肪和虾味氨基酸含量随着对虾规格的增大而减少,蛋白质、灰分、氨基酸、必需氨基酸、半必需氨基酸和鲜味氨基酸总量随着对虾规格的增大而增加;相同规格的凡纳滨对虾中,淡水养殖对虾的含水量、脂肪含量和不饱和脂肪酸含量较高,蛋白质、灰分、鲜味氨基酸总量和虾味氨基酸偏低。综合指标表明,海水养殖凡纳滨对虾的营养价值随着规格的增大而提高;在蛋白质、灰分、鲜味氨基酸和虾味氨基酸含量上,海水养殖对虾高于同一规格的淡水养殖对虾。     

低胆红素培植牛黄的免疫药理作用研究

本文通过免疫药理作用的实验研究证明，低胆红素培植牛黄（胆红素含量为８．７％），按１ｇ／ｋｇｐｏ给药对小鼠溶菌酶活力，单核巨噬细胞系统的吞噬功能，ＤＮＣＢ所致的迟发型过敏反应以及Ｂ淋巴细胞功能均有显著的抑制作用。     

培植羊黄的扫描电镜观察

对培植羊黄进行了扫描电镜观察,并与培植牛黄,天然牛黄进行比较。三种黄的超微结构相似,分层明显,都具有丝网状结构,黄的基质部分为硫酸化多糖粘蛋白所构成。     

不同苗龄“威廉斯B6”香蕉组培生根苗生产同批假植苗的研究

结合生根培养苗龄、假植基质等因素,研究了不同假植基质对不同苗龄香蕉组培生根苗假植成活率以及对假植苗假茎高度、叶片数、根系数、根系长度等生长指标的影响;同时研究了不同苗龄的瓶苗于不同季节在椰糠+珍珠岩中假植成活率的变化情况。结果表明:相同苗龄的香蕉组培生根苗在不同基质中假植成活率有显著差异,在椰糠+珍珠岩的混合基质中假植成活率最高;不同苗龄也是如此;结合假茎高度、叶片数、根系数、根系长度等生长指标认为椰糠+珍珠岩为最优假植基质,在这一基质中假植成活率可达92%～99%,能够满足不同苗龄香蕉组培生根苗在不同季节对假植基质的要求。     

Timing of spawning in cultured and wild Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) in the River Vosso, Norway

Spawning time and size of wild and nonnative cultured female Atlantic salmon (Salmo salar L.) were studied in the River Vosso, Norway. Cultured immigrants were smaller, and peak spawning occurred 21 and 26 days earlier relative to wild salmon in 1991 and 1992, respectively. It was calculated that hatching and initial feeding of offspring of cultured females peaked 12 and 9 days earlier in spring 1992 and 19 and 8 days earlier in spring 1993 relative to offspring of wild females. Spawning time of the cultured salmon is similar to most Norwegian salmon populations and to cultured fish in other streams. In the River Vosso it also overlaps with that of brown trout (Salmo trutta L.), which may increase hybridization. The early time of breeding in nonnative fish indicates local adaptation in this trait in wild salmon. The consequences of early breeding of cultured fish are unpredictable in relation to spring temperatures but probably reduce their relative breeding success. The smaller size may also reduce the success of cultured females.