丽格海棠的离体快繁研究

组培快繁技术研究结果表明:适宜丽格海棠叶片诱导芽分化的培养基为MS 6 BA0 50mg/L NAA1 00mg/L;适宜丛生芽继代增殖的培养基为MS 6 BA0 25mg/L NAA1 00mg/L;适宜丽格海棠试管苗生根的培养基为1/2MS,其生根率为100%。     

信阳板栗低产林综合改造技术的研究

信阳市板栗生产中普遍存在产量低,品质差问题,本文通过对板栗低产原因的分析,为开发板栗资源,提出了优质高产栽培的重要途径和措施。     

Somatic embryo culture for propagation,artificial seed production,and conservation of sawara cypress (<Emphasis Type="Italic">Chamaecyparis pisifera</Emphasis> Sieb. et Zucc.)

 Somatic embryogenesis in Chamaecyparis pisifera Sieb. et Zucc. was initiated from immature seeds collected from the end of June to early July. Mass propagation through adventitious shoot bud production from somatic embryo culture on Woody Plant (WP) medium and artificial seed production using sodium alginate was achieved. A high bud forming index value (25.8) was obtained on medium supplemented with 1 μM 6-benzylaminopurine. The conversion rates from artificial seeds under aseptic and nonaseptic conditions were 60%–100% and 10%–12%, respectively. For germplasm conservation, somatic embryos and embryogenic cells were successfully stored at 4°C (medium-term storage) and in liquid nitrogen for long-term storage. Received: December 21, 2001 / Accepted: August 1, 2002 Acknowledgments This work was supported in part by the Japan Science and Technology Corporation and in part by a Grant for Research for the Future Program from the Japan Society for the Promotion of Science. Correspondence to:E. Maruyama     

Liquid culture and transformation of hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.)

Hinoki cypress (Chamaecyparis obtusa) is one of the most important timber resource forest trees in Japan. Because seed production from a seed orchard of hinoki cypress is not constant every year, micropropagation from a limited amount of material is useful. Up to now, the conventional tissue culture method using solid medium has been used. Here a new method using liquid culture in tubes rotated vertically is described. Shoot primordium of hinoki cypress was inoculated in Campbell and Durzan’s (CD) liquid medium containing different cytokinins (6-benzylaminopurine (BAP), Zeatin, thidiazurone (TDZ)), and the container tubes were rotated vertically around the axis at 2 times / min. Culture room temperature was 25°C and light condition was 16 h photoperiod per day of fluorescent lamps. Zeatin at 1μM concentration was the best for maintaining the shoot primordium production and TDZ induced callus on the surface of the shoot primordia. After shoot primordium multiplication in the liquid culture, they were transplanted to agar medium for shoot elongation. A high concentration of agar (up to 16 g/L) or AVF (anti vitrification factor from Dr. Nairn, 1995) was effective to prevent vitrification of the shoots. Transformation of shoot primordium was done using particle bombardment with vectors containingβ-glucuronidase (GUS) gene or herbicide resistance gene (bar). Positive result for transient transformation was observed with the histo-chemical study for transformation with GUS. Integration of a useful herbicidebar gene into the shoot primordium culture system was also tried and stably transformed plants were obtained. This is the first report of stable transformation of Japanese conifer using practically useful gene. The generous supply of AVF-B from Dr. B.J. Nairn, Tasman Forestry, NZ is also appreciated.     

榉树茎尖的培养

选用成年榉树 Schneideriana 的当年生嫩枝作为外植体,经常规消毒后,接种于附加不同质量浓度的激素BA、NAA、2,4-D的MS培养基上,诱导茎尖萌芽.结果表明:诱导芽萌发的最适宜的培养基为MS BA( 1.0 mg·L-1),继代培养后,在每个芽的基部又可长出3～7个小芽;小芽在MS BA(0.5 mg·L-1) CH(500 mg·L-1)培养基上培养30～40 d,即可长成小植株;在 1/2MS IBA(0.5 mg·L-1)的生根培养基上培养3～4周后可形成正常植株.     

云南楤木组织培养快速繁殖试验

利用组织培养技术对云南木进行了组培快繁技术研究。筛选出适合云南木组培各阶段的适宜激素浓度和配比,分别为:启动培养基MS+6-BA 0.18mg/L+IBA 0.4mg/L;增殖培养基MS+6-BA1.2 mg/L+IBA 0.5mg/L;生根培养基1/2MS+IBA 3.0mg/L。并阐明了继代次数与增殖系数和生根率的关系,提出云南木继代次数以8代左右为宜,8代之后应进行生根培养。     

紫叶黄栌观赏特性及快繁技术研究

以紫叶黄栌的当年生嫩枝茎段为外植体进行簇生芽诱导,研究结果表明,诱导簇生芽时,在MS 6-BA2．0 mg／L NAA0．2 mg／L培养基上的诱导效果较好,分化率达100％;继代培养时,以MS 6-BA1．0 mg／L KT 0．5 mg／L NAA0．1 mg／L培养基增殖倍数较高,达到11．8倍;再生苗在1/2MS NAA0．1 mg／L的培养基上生长良好;在炼苗中,移栽基质以蛭石 森林土较好,可促进小苗后期生长。     

山西省的绿篱植物资源与利用

对山西省现有23种主要绿篱植物，分别从形态、习性、繁殖、利用等方面作了介绍，并就一般绿篱的分类，及通用的栽培方法作了简要介绍，以便为绿篱植物资源的开发利用和城乡环境的绿化、美化提供依据。     

改进马尾松育苗方法的试验

对马尾松采用控制苗木密度、芽苗截根移栽和接种菌根菌等技术进行育苗试验，结果表明：苗木密度应控制在１５０株／ｍ２，芽苗截根以保留主根长度１／２为宜；采用厚环乳牛肝菌进行人工接种效果较好。     

Slash pine (Pinus elliottii Engelm.) somatic embryogenesis II. Maturation of somatic embryos and plant regeneration

Maturation of slash pine (Pinus elliottii Engelm.) somatic embryos was achieved using two protocols, each starting with a different agar incubation step to deplete plant growth regulators (PGRs) used in previous cultural steps. Strength of maturation medium (single vs. double) was found important in the first protocol to develop normal, mature embryos. In the second protocol, abscisic acid (ABA) concentrations (0, 15 and 30 M) and carbohydrate sources were tested for embryo maturation. Thirty M ABA and 6% maltose were deemed the best combination. Embryo germination was accomplished in a continuously lighted environment and embryos receiving a cold pretreatment (4 °C in darkness for 16 days) germinated better than embryos which did not receive cold pretreatment. With a survival rate of 33% after acclimation in a mist system, more than 25 plants from somatic embryos have been established in a greenhouse. Incompletely germinated embryos (lacking roots) were rooted via adventitious rooting techniques and subsequently established in the greenhouse. All established plants obtained from somatic embryogenesis appear normal in morphology.