利用分子标记辅助选育白菜薹复等位基因型雄性不育系

为解决白菜薹杂种优势利用中的杂交制种手段问题,以大白菜复等位基因型雄性不育系为不育源,设计定向转育方案,采用连续回交的方法转育不育性和农艺性状,同时利用分子标记辅助选择目标基因型植株,向白菜薹自交系中转育不育基因,创制白菜薹雄性不育系。通过26对SSR引物的筛选鉴定,获得与显性雄性不育基因Ms紧密连锁、且同样可以标记同一位点恢复基因Msf和可育基因ms的分子标记GSSR1。经过3个世代的回交转育,创制出了具有100%不育度和100%不育株率的白菜薹复等位基因型雄性不育系BGMS-3。以BGMS-3为母本,与6个白菜薹自交系杂交,筛选出1个强优势组合C3。     

Availability of Cadmium in some Phosphorus Fertilizers to Field-Grown Lettuce

Although evaluations of the availability of cadmium (Cd) contaminants in phosphate fertilizers have been made, few have examined the transfer efficiency of Cd from fertilizers to plants, especially under field conditions. This 2-year field study determined the transfer of added Cd to lettuce (Lactuca sativa L.) (Royal Green) from a western phosphate rock (PR) and a triple superphosphate (TSP) as affected by liming and rate of fertilizer (or Cd) input. A readily soluble Cd salt, CdCl₂, was included in the study for comparison. The cumulative amounts of Cd added from the fertilizers and CdCl₂ over the 2-year period ranged from 0 to 1440 g ha^–1. Lettuce yield increased with increasing TSP rates, but was unaffected by PR. Significant (P < 0.01) effects of Cd source and rate, lime, and year were found on Cd accumulation by lettuce. The transfer of the added Cd was consistently higher for CdCl₂ than for the fertilizers regardless of lime rate. A contrasting year effect was also found between the two P fertilizers. In the second year of application, the Cd transfer efficiency increased in the soil treated with the PR, but decreased in the soil treated with the TSP. The Cd transfer efficiency for the plant was better measured with DTPA–Cd (r ²= 0.78 – 0.80) or CaCl₂–Cd (r ²= 0.57 – 0.76) than with soil total Cd (r ²= 0.39 to 0.54) across all Cd sources and lime rates. This is because DTPA–Cd or CaCl₂–Cd reflected the influences of the amount of Cd added, Cd source, and lime rate on Cd accumulation by the plant better than did the soil total Cd. Of the amount of Cd added from the fertilizers an average of 1.0% or less was accumulated in the harvested lettuce tissue. Applications of the fertilizers at high rates could result in increased Cd accumulation in the soil over time.     

黄河小浪底水库蓄水前后库周土地覆被变化研究

选取黄河小浪底水库库周1993年和2000年TM遥感影像进行土地覆被变化分析,绘制出蓄水前后的土地利用现状图,计算出土地覆被变化的转移矩阵和景观结构的控制指标,分别从土地利用类型、空间景观结构特征及土地变化过程等方面进行了研究.研究结果表明:小浪底水库的施工和蓄水对库周的土地利用变化和空间景观结构的改变没有太大影响;由于林地向耕地和其它土地覆被类型转变,使得1993～2000年的模地类型由有林地向耕地转化     

Study on Analysis Methods for Rock Slope FoundationStability of the Highrise Building

Based on the practical project a study on analysis methods for rock slope foundation stability of highrise-building project criterion transfer coefficient method, rotation limit equilibrium method (Sweden slices method) and finite element method was carried out.The authors found that safety factor of criterion transfer coefficient method is larger than that of rotation limit equilibrium method, but is similar to the results of finite element method. So, when rockslope foundation stability of highrise building is to be analysed,the rotation limit equilibrium method is suggested to use.     

羊胚胎移植B超监测技术及其影像的研究

利用AMI-900型兽用B超,对羊胚胎移植进行了全程监测．主要包括供、受体羊选择前的普查,供体超排效果的监测,受体妊娠及胚胎发育监测,子宫、卵巢恢复状况监测等．根据所得资料和经验,总结出了羊胚胎移植中的B超监测技术及其声像图表现．     

影响胚胎移植效益的因素分析

利用系统工程层次分析的方法，将影响胚胎移植效益的各因素给以定量化的研究，结果表明，技术因素对胚胎移植效益影响最大，占６６．９４％。在技术因素中，超排效果和移植成功率影响最大，对总效益的影响分别为３２．９９％和１５．６２％。     

优质早稻高产综合配套栽培技术研究

根据江西省试种和推广的优质早籼稻品种的生育特性，分别围绕育秧技术、施肥技术、无公害生产以及栽培条件对品质影响等方面进行了研究。并根据研究结果和已有推广的技术，编制了“优质早稻高产简化栽培技术模式图”，边试验、边推广，取得了显著的经济和社会效益。     

地域文化在城市公园设计中的应用

本文针对当前国内的城市公园设计中地域文化缺失的问题,以地域文化和城市公园关系为研究对象,通过查阅大量文献,分析了地域文化在城市公园设计中的选择方法和表达途径。在设计过程中,只有把能够体现城市历史文化、再现风土人情和民俗习惯的地域文化融合于城市公园景观中,才能体现一个城市区别于其它城市的风格。     

A data transfer method for improving seed identification of maize (Zea mays) haploid breeding based on genetic similarity

Maize haploid breeding technology is able to identify haploid seeds non‐destructively, rapidly and at low cost with the help of Near‐infrared (NIR) spectral analysis. However, due to the hybridization of numerous parents and the low production rate of haploid, the haploid data collection becomes a burden for engineering this technology. Biologically, there are considerable similarities between the progeny of the same female parent and different male parents. Based on this advantage, similar spectral data can be transferred when the NIR technology is employed. A revised method of Transfer adaptive boost (TrAdaBoost) is proposed to improve identifying for the backpropagation neural network (BPNN) classifier. To avoid the negative transfer, a screening thresh is used to select out similar data, and the amount of these data are limited to join current training. The results show that the identification performances are improved significantly when the data amount is small. This method shows a high ability to make the seed identification more convenient for engineering maize haploid breeding.