试论农业高职学院为“三农”服务的文化策略

根据文化人类学对文化的三分传统,农业高职学院作为一个相对独立的文化体系,其社会服务职能具有层次性和渐进性。从＂物质＂、＂制度＂和＂精神＂三维度对农业高职学院服务职能进行探讨,可以总结出农业高职学院为＂三农＂服务的三大文化策略,即＂适应-融入＂策略、＂整合-提升＂策略和＂创新-引领＂策略。     

境外网络游戏的软权力作用分析

境外网络游戏是软权力在文化领域的重要载体之一,正日益凸显其重要地位,大多是通过网络游戏的内容和背景展现出来,意在增加本国经济效益的同时,以达到能够吸引不同国家和地区的更多玩家,灌输和宣传本国的思想和文化,使之成为配合其各国软权力战略实施的双重作用。面对这种新的文化渗透模式,研究如何应对境外网络游戏的隐性影响,是目前研究的主要课题。     

Decreased platelet function in Cavalier King Charles Spaniels with mitral valve regurgitation

With aggregometry, increased platelet activity has been reported in Cavalier King Charles Spaniels (CKCS) without mitral regurgitation (MR). In contrast, dogs with MR have been found to have decreased platelet activity. The purpose of this study was to test an easy bedside test of platelet function (the Platelet Function Analyzer [PFA-100]) to see if it could detect an increase in platelet activity in CKCS without MR and a decrease in platelet activity in CKCS with MR. This study included 101 clinically healthy dogs > 1 year of age: 15 control dogs of different breeds and 86 CKCS. None of the dogs received medication or had a history of bleeding. The PFA-100 evaluates platelet function in anticoagulated whole blood under high shear stress. Results are given as closure times (CT): the time it takes before a platelet plug occludes a hole in a membrane coated by agonists. The CT with collagen and adenosine-diphosphate as agonists was similar in control dogs (median 62 seconds; interquartile interval 55-66 seconds) and CKCS with no or minimal MR (55; 52-64 seconds). The CT was higher in CKCS with mild MR (regurgitant jet occupying 15-50% of the left atrial area) (75; 60-84 seconds; P = .0007) and in CKCS with moderate to severe MR (jet > 50%) (87: 66-102 seconds; P < .0001). CKCS with mild, moderate, and severe, clinically inapparent MR have decreased platelet function. The previous finding of increased platelet reactivity in nonthrombocytopenic CKCS without MR could not be reproduced with the PFA-100 device.     

牛、羊卵母细胞孤雌激活的研究

本试验主要比较了离子霉素、电脉冲两种方法激活牛、羊体外成熟卵母细胞的效率。两种激活方法中。牛胚胎卵裂率无显著差异（90．61％对94．40％，P〉0．05），而离子霉素激活胚胎的囊胚发育率极显著高于电激活方法（12．3％对2．4％，P〈0．01）。两种方法对羊胚胎的研究中，羊胚胎卵裂率无显著差异（72．4％对77．4％，P〉0．05）。但是离子霉素激活胚胎的囊胚发育率显著高于电激活方法（3．67％对10．40％，P〈0．05）。本试验中还比较了用化学激活法（离子霉素）激活牛体外成熟卵母细胞后，用SOFaa体系培养，换液与不换液对孤雌激活胚胎体外发育的影响。结果表明：在第4天不换液的胚胎卵裂率和囊胚率极显著高于换液的胚胎（11．64％对3．49％。P〈0．01）。     

我国文化体制改革的人本原则

我国文化事业体制和机制的僵化导致了一系列的问题。发展我国文化事业必须坚持“以人为本”的原则，即遵循文化企事业单位和文化市场的发展规律，通过人本身的知识、能力和创新技术创造出文化产品和服务来充分满足人的精神文化需求，从而最终达到人的全面发展。     

氮、磷和钾肥施用量对有机生态型无土栽培温室黄瓜产量影响的研究

采用二次回归饱和D-最优试验设计方法研究了固态N、P和K肥施用量对日光温室有机生态型无土栽培春茬黄瓜产量的影响,建立了N、P和K肥施用量与早、中、后期黄瓜产量形成之间的数学模型.试验结果表明:N、P及K对黄瓜的早期产量影响极显著,产量区间为2 400～3 260 g/小区的最佳施肥量(单位:g/株/次)为N 0.83,P2O5 0.37,K2O 0.86;P、K是影响黄瓜中期产量的主要因素,产量区间为6 400～9 200 g/小区的最佳施肥量分别为N 0.66,P2O5 0.33,K2O 0.90;黄瓜生长后期,N肥对黄瓜产量的影响极其显著,产量区间为2 000～2 940 g/小区的最佳施肥量为N 0.61,P2O5 0.40,K2O 0.94.     

Preliminary experiments on mechanical stretch-induced activation of skeletal muscle satellite cells in vivo

We have shown in vitro that mechanical stretch triggers activation of quiescent satellite cells of skeletal muscle to enter the cell cycle through an intracellular cascade of events including nitric oxide (NO) synthesis that results in the release of hepatocyte growth factor (HGF) from its extracellular association and its subsequent presentation to signaling receptors. In order to explore the activation mechanism in vivo, stretch experiments were conducted in the living animal using our suspension model developed. This system used the weight of the hind portion of rats to stretch the inside muscles of the left hind limb suspended for a period of 0.5–2.0 h. At the end of the stretch period, the rats received an intraperitoneal injection of bromodeoxyuridine followed by immunocytochemistry for its incorporation as an index of satellite cell activation in vivo. Depending on the period of stretch, bromodeoxyuridine labeling was increased significantly over the contralateral unstretched leg or control muscle from untreated rats. A stretched muscle extract prepared from the 2 h stretched tissue by incubating it in PBS, showed the active form of HGF as revealed by immunoblotting and it could stimulate the activation of unstretched satellite cells. Also, administering NO synthase inhibitor L‐NAME prior to muscle stretch abolished the stretch activation of satellite cells. Therefore, the results from these experiments demonstrate that stretching muscle triggers NO synthesis and HGF release, which could activate satellite cells in vivo.     

序贯培养法对猪孤雌激活胚胎发育能力的影响

经体外成熟、孤雌激活和培养获得猪胚胎,研究了不同培养体系、共培养体细胞和序贯培养对猪孤雌激活胚胎发育的影响。试验表明:孤雌激活卵母细胞在SOF 10?S培养体系中分裂效果最好,添加胎牛血清的NCSU-23和颗粒细胞对胚胎发育有促进作用,培养6d后发育到桑囊胚的比率增加(P<0.05)。在序贯培养的前3d,SOF培养基(不含葡萄糖)和颗粒细胞对胚胎的发育有促进作用,分裂率(P<0.05)和突破4细胞阻滞的数目显著增加,在培养的后3d,添加胎牛血清的NCSU-23和输卵管上皮细胞能支持较多胚胎发育到桑囊胚,桑囊胚的发育率为(59.5±3.2)%(P<0.05)。结果表明,SOF培养基和颗粒细胞 添加胎牛血清的NCSU-23和输卵管上皮细胞的序贯培养系统能较好的促进胚胎的发育。     

Status of black grass (Alopecurus myosuroides) resistance to acetyl-coenzyme A carboxylase inhibitors in France

We assessed the contributions of target site‐ and non‐target site‐based resistance to herbicides inhibiting acetyl‐coenzyme A carboxylase (ACC) in Alopecurus myosuroides (black grass). A total of 243 A. myosuroides populations collected across France were analysed using herbicide sensitivity bioassay (24 300 seedlings analysed) and ACC genotyping (13 188 seedlings analysed). Seedlings resistant to at least one ACC‐inhibiting herbicide were detected in 99.2% of the populations. Mutant, resistant ACC allele(s) were detected in 56.8% of the populations. Among the five resistant ACC alleles known in A. myosuroides, alleles containing an isoleucine‐to‐leucine substitution at codon 1781 were predominant (59.5% of the plants containing resistant ACC alleles). Comparison of the results from herbicide sensitivity bioassays with genotyping indicated that more than 75% of the plants resistant to ACC‐inhibiting herbicides in France would be resistant via increased herbicide metabolism. Analysis of herbicide application records suggested that in 15.9% of the populations studied, metabolism‐based resistance to ACC‐inhibiting herbicides was mostly selected for by herbicides with other modes of action. Our study revealed the importance of non‐target site‐based resistance in A. myosuroides. Using herbicides with alternative modes of action to control populations resistant to ACC‐inhibiting herbicides, the recommended management approach, may thus be jeopardised by the widespread occurrence of metabolism‐based resistance mechanisms conferring broad‐spectrum cross‐resistance.     

Significance of leaf infection by Botrytis cinerea in stem rotting of tomatoes grown in non-heated greenhouses

The most serious symptom of Botrytis cinerea in tomatoes grown in greenhouses is stem rotting. Lesions on the stem may result from direct infection or from progression of the rot along infected leaves, until infection approaches the stem. In a set of experiments conducted in commercial greenhouses, an experimental greenhouse and growth chambers, the significance of the two types of stem infections was studied. In non-heated greenhouses most of the stem lesions originated from progression of the pathogen along infected petioles. The rate at which B. cinerea had progressed on infected petioles was 0.3–0.5 cm/day, an average of ca. 6 weeks was needed for a leaf infection to approach the stem. Application of Trichoderma harzianum T39 extended this time by 1–2 weeks and application of chemical fungicides by 3 weeks. Influence of the environment on the progression of B. cinerea along infected petioles was then determined. Within range of 5–30 °C, the higher the temperature, the more rapid was the rate of disease progression. The fungus progressed more rapidly on tomato petioles incubated at high vapour pressure deficit (VPD) rather than at low VPD. The source-sink relationship of the plant governed the rate of B. cinerea progression along the petioles as well: it was more rapid when the source was restricted (by shading) and slower when the sink was restricted (by removal of flowers and small fruits). The possibility that sanitation of infected leaflets would reduce the incidence of stem rotting was examined in two experiments. In plots not treated with a fungicide, the sanitation treatment substantially decreased the incidence of stem lesions and this treatment was as effective as weekly application of chemical fungicides.