In-field management of corn cob and residue mix: Effect on soil greenhouse gas emissions

In-field management practices of corn cob and residue mix (CRM) as a feedstock source for ethanol production can have potential effects on soil greenhouse gas (GHG) emissions. The objective of this study was to investigate the effects of CRM piles, storage in-field, and subsequent removal on soil CO₂ and N₂O emissions. The study was conducted in 2010–2012 at the Iowa State University, Agronomy Research Farm located near Ames, Iowa (42.0°′N; 93.8°′W). The soil type at the site is Canisteo silty clay loam (fine-loamy, mixed, superactive, calcareous, mesic Typic Endoaquolls). The treatments for CRM consisted of control (no CRM applied and no residue removed after harvest), early spring complete removal (CR) of CRM after application of 7.5 cm depth of CRM in the fall, 2.5 cm, and 7.5 cm depth of CRM over two tillage systems of no-till (NT) and conventional tillage (CT) and three N rates (0, 180, and 270 kg N ha⁻¹) of 32% liquid UAN (NH₄NO₃) in a randomized complete block design with split–split arrangements. The findings of the study suggest that soil CO₂ and N₂O emissions were affected by tillage, CRM treatments, and N rates. Most N₂O and CO₂ emissions peaks occurred as soil moisture or temperature increased with increase precipitation or air temperature. However, soil CO₂ emissions were increased as the CRM amount increased. On the other hand, soil N₂O emissions increased with high level of CRM as N rate increased. Also, it was observed that NT with 7.5 cm CRM produced higher CO₂ emissions in drought condition as compared to CT. Additionally, no differences in N₂O emissions were observed due to tillage system. In general, dry soil conditions caused a reduction in both CO₂ and N₂O emissions across all tillage, CRM treatments, and N rates.     

日光温室中荷8号黄瓜育苗嫁接及管理技术

保护地生产已经成为喀左县农民增收的重要产业,本文详细总结了日光温室栽培中荷8号黄瓜的育苗、嫁接及管理技术,供同类地区参考与借鉴。     

重组猪抑制素蛋白质中毒素残留检测及其理化稳定性

为探索纯化的重组猪抑制素蛋白质毒素残留情况以及重组猪抑制素蛋白质稳定性,本研究利用鲎试剂、气相色谱和SDS-PAGE方法对重组猪抑制素蛋白质中的细菌内毒素、β-巯基乙醇的残留量及其蛋白质在不同温度、极端p H值和反复冻融条件下的稳定性进行检测。结果显示,经过3次等电点沉淀洗涤后,重组猪抑制素蛋白质中细菌内毒素和β-巯基乙醇的残留量大幅减少。通过在不同温度下的处理发现,重组猪抑制素蛋白质在4℃环境下较稳定,可以长期保存;但在37℃时,稳定性快速下降,随着温度上升而降解加速。通过极端p H值和反复冻融试验发现,重组猪抑制素蛋白质对极端p H值和反复冻融具有较好的耐受性。     

中国和欧盟动物源性产品中药物残留限量差异性分析(一)

将中国和欧盟动物源性产品中药物残留标准进行了对比,同时结合TBT/SPS预警数据库,找出二者差异性,并提出了改进建议。     

供氮方式对冬马铃薯氮肥利用效率及氮素去向的影响

以马铃薯费乌瑞它为试材,采用田间微区~(15)N示踪技术,研究施N量160kg·hm~(-2)全部基施(T1)、55%基施+45%在齐苗期追施(T2)、55%基施+30%在齐苗期追施+15%在现蕾期追施(T3)3种方式,对冬马铃薯氮肥利用效率及去向的影响。结果表明:马铃薯吸收的N约46%~52%来源于当季施用的氮肥,48%~54%来自土壤和种薯;肥料N利用率为35.16%~39.99%,残留率为47.71%~51.78%,损失率为8.23%~15.50%。3种施氮方式下,肥料N主要残留在0~15cm土层。随施氮时间后移,肥料N残留在0~15cm土层呈上升趋势,在15~45cm土层呈下降趋势。施氮方式对马铃薯干物质积累总量和块茎干物质积累量影响不明显,但T3肥料N利用率、肥料N残留率明显大于T1、T2。因此,综合经济效益和环境效益,T3施氮方式的效果较为理想。本研究为马铃薯氮素养分的有效管理提供了指导依据。     

Effects of cover crop growth and decomposition on the distribution of aggregate size fractions and soil microbial carbon dynamics

Although the effects of cover crops (CC) on various soil parameters have been fully investigated, less is known about the impacts at different stages in CC cultivation. The objective of this study was to quantify the influence of CC cultivation stages and residue placement on aggregates and microbial carbon (C_mic). Additionally, the influence of residue location and crop species on CO₂ emissions and leached mineralized nitrogen (N_min) during the plant degradation period was also investigated. Within an incubation experiment, four CC species were sown in soil columns, with additional columns being kept plant‐free. After plant growth, the columns were frozen (as occurs in winter under field conditions) and then incubated with the plant material either incorporated or surface‐applied. With CC, concentrations of large and medium macroaggregates were twice that of the fallow, confirming positive effects of root growth. Freezing led to a decrease in these aggregate size classes. In the subsequent incubation, the large macroaggregates decreased far more in the samples with CC than in the fallow, leading to similar aggregate size distributions. No difference in C_mic concentration was found among the CC cultivation stages. CO₂ emissions were roughly equivalent to the carbon amounts added as plant residues. Comparison of columns with incorporated or surface‐applied residues indicated no consistent pattern of aggregate distribution, CO₂ emission or C_mic and N_min concentrations. Our results suggest that positive effects of CC cultivation are only short term and that a large amount of organic material in the soil could have a greater influence than CC cultivation.     

刺参生殖腺发育的生物学零度和有效积温研究

[目的]为刺参的繁殖以及工厂化人工育苗提供理论基础。[方法]以渤海湾地区刺参为试验材料,研究渤海湾地区刺参生殖腺发育生物学零度及其性成熟的有效积温。[结果]渤海湾地区刺参生殖腺发育的生物学零度为6.14℃,而有效积温为800.19℃·d。[结论]该研究在渤海湾地区刺参育苗生产中具有重要的参考价值。     

黄瓜低霜霉威残留性相关基因SDH克隆及表达

通过Solexa技术和比较基因组学方法鉴定霜霉威胁迫下黄瓜果实差异表达基因,筛选黄瓜低霜霉威残留性相关基因SDH。研究利用PCR克隆获得SDH基因全长,命名为Cs SDH。构建亚细胞定位表达载体,利用农杆菌浸润法注射烟草叶片后激光共聚焦显微镜下观察;分别用荧光定量RT-PCR方法和酶联免疫法研究高、低农残品系D9320和D0351中Cs SDH基因在农药霜霉威处理后,不同时间点、不同组织部位时空表达特征和琥珀酸脱氢酶活性。结果表明,Cs SDH基因在烟草叶片细胞中被定位在细胞膜上。黄瓜低农残品种D0351中,Cs SDH基因在果实受霜霉威胁迫后反应迅速,主要在处理前期响应表达强烈,琥珀酸脱氢酶活性显著增加,且该基因表达量显著高于其在高农残品种D9320中表达量。Cs SDH果、叶中表达量较茎中高,且各组织部位表达量高低顺序稳定,为叶果茎。黄瓜高农残品种D9320中,Cs SDH基因表达量在0.5、1、3、12 h呈现上调表达,6、9 h基因表达量差异不显著,48 h出现上调表达高峰。说明克隆得到的Cs SDH基因属农药处理前期响应基因,且该基因可能在降低黄瓜农药残留过程中发挥重要作用。试验通过研究黄瓜琥珀酸脱氢酶基因(SDH)在霜霉威胁迫下表达情况,为挖掘黄瓜低农药残留性功能基因以及探明其作用分子机制奠定基础。     

Do combined applications of crop residues and inorganic fertilizer lower emission of N2O from soil?

Emissions of N₂O were measured following addition of ¹⁵N‐labelled residues of tropical plant species [Vigna unguiculata (cowpea), Mucuna pruriens and Leucaena leucocephala] to a Ferric Luvisol from Ghana at a rate of 100 mg N/kg soil under controlled environment conditions. Residues were also applied in different ratio combinations with inorganic N fertilizer, at a total rate of 100 mg N/kg soil. N₂O emissions were increased after addition of residues, and further increased with combined (ratio) applications of residues and inorganic N fertilizer. However, ¹⁵N‐N₂O production was low and short‐lived in all treatments, suggesting that most of the measured N₂O‐N was derived from the applied fertilizer or native soil mineral N pools. There was no consistent trend in magnitude of emissions with increasing proportion of inorganic fertilizer in the application. The positive interactive effect between residue‐ and fertilizer‐N sources was most pronounced in the 25:75 Leucaena:fertilizer and cowpea:fertilizer treatments where 1082 and 1130 mg N₂O‐N/g residue were emitted over 30 days. N₂O (log_e) emission from all residue amended treatments was positively correlated with the residue C:N ratio, and negatively correlated with residue polyphenol content, polyphenol:N ratio and (lignin + polyphenol):N ratio, indicating the role of residue chemical composition in regulating emissions even when combined with inorganic fertilizer. The positive interactive effect in our treatments suggests that it is unlikely that combined applications of residues and inorganic fertilizer can lower N₂O emissions unless the residue is of very low quality promoting strong immobilisation of soil mineral N.     

凉茶渣的黑曲霉发酵工艺和抗氧化活性研究

为促进凉茶渣在畜牧业中的资源化利用,本研究以黑曲霉为菌种固态发酵凉茶渣,首先在单因素试验条件下考察了时间、温度、含水量、浸泡液pH、氮源和碳源对凉茶渣降解率和产物pH的影响;再根据单因素试验结果和规模化生产实际需要,以4%硫酸铵为氮源,2%葡萄糖为碳源,以降解率为考察指标,通过正交试验优化发酵工艺;并通过检测超氧自由基清除率、羟基自由基清除率和1,1-二苯基-2-三硝基苯肼（DPPH）自由基清除率,评价凉茶渣在最优工艺条件下发酵前后抗氧化活性的变化。结果发现,含水量为60%,浸泡液pH为9.0,31℃发酵168 h是凉茶渣的最佳发酵工艺参数。最佳工艺条件下凉茶渣的降解率为25.23%,发酵产物pH为4.53。当凉茶渣发酵前水提液浓度为24 mg/mL时,超氧自由基清除率为43.56%,羟基自由基清除率为47.06%,DPPH自由基清除率为90.71%;当最优条件下发酵产物水提液浓度24 mg/mL时,超氧自由基清除率为30.77%,羟基自由基清除率为95.63%,DPPH自由基清除率为87.36%。本试验结果表明,黑曲霉是适宜的凉茶渣发酵菌种,且凉茶渣经过黑曲霉发酵后具有良好的抗氧化活性。