基于田间空气中病菌孢子浓度的小麦白粉病病情估计模型研究

2014和2015两年度利用Burkard定容式孢子捕捉器监测了高感小麦白粉病品种京双16种植区和中感品种众麦2号种植区田间空气中的病菌孢子浓度变化动态,同时利用小型气象站监测了田间的气象因子,通过分析京双16和众麦2号种植区空气中孢子浓度与空气温度、湿度、降雨、风速和太阳辐射率的相关性,发现空气中的孢子浓度主要与空气温度呈显著的正相关性(r0.348 3,P0.05)。在此基础上,分别分析了田间病情与调查日期前累积孢子浓度、一周前累积孢子浓度、前一周累积孢子浓度和当周累积孢子浓度的关系,结果表明,中感品种众麦2号田间病情与累积孢子浓度的关系均呈指数关系,其中田间病情与调查日期前累积孢子浓度或一周前累积孢子浓度的拟合效果最好,而感病品种京双16的田间病情与累积孢子浓度多呈对数关系,其中病情指数与一周前累积孢子浓度的拟合效果最好。     

The Influence of Carbon Dioxide or Ozone Concentration on Growth and Assimilate Partitioning in Seedlings of Nine Conifers

Abstract

Seedlings of nine different conifers were exposed to 355 and 730 μmol mol-1 CO₂, or low (> 15 nmol mol^?1) and elevated 0₃ concentration (70 nmol mol^?1) for 81–116 days. The experiments were conducted in growth chambers placed in a greenhouse. Increased CO₂ concentration enhanced the mean relative growth rate (RGR) and total plant dry weight by 4 and 33% in Larix leptolepis, by 4 and 38% in Larix sibirica, by 7 and 47% in Picea glauca and by 3 and 16% in Picea sitchensis, respectively. The growth rates and dry weights of Pimis contorta, Pinus mugo and Pseudotsuga menziesii were not significantly affected. Carbon dioxide enrichment enhanced RGR of two provenances of Picea abies by 4 and 6%, respectively, while a third provenance was unaffected. In Pimis sylvestris, only the RGR of one of three provenances was stimulated by CO₂ enrichment (4%).

After two growth seasons CO₂ enrichment enhanced RGR and total plant dry weight by 11 and 35% in Picea abies and by 12 and 36% in Pinus sylvestris, respectively. Elevated CO₂ decreased the shoot:root ratio in Larix leptolepis, and decreased the needlerstem ratio in Picea glauca, but increased it in Pseudotsuga menziesii.

Elevated O₃ significantly decreased the plant dry weight in Picea sitchensis, Pseudotsuga menziesii and in one of three provenances of Pinus sylvestris, while the other species and provenances were unaffected. Increased O₃ concentration increased the shoot:root dry weight ratio in one of three Picea abies provenances, in all three Pinus sylvestris provenances and in Pinus contorta. The needle:stem ratio was enhanced by O₃ in seven of the nine species. The O₃ exposure caused chlorosis of needles in all species except Pseudotsuga menziesii.     

Effects of different dl‐selenomethionine and sodium selenite levels on growth performance,immune functions and serum thyroid hormones concentrations in broilers

This trial was conducted in a 2 × 3 + 1 factorial arrangement based on a completely randomized design to evaluate the effects of different dl ‐selenomethionine (dl ‐Se‐Met) and sodium selenite (SS) levels on growth performance, immune functions and serum thyroid hormones concentrations in broilers. A total of 840 Ross 308 broilers (7 days old) were allocated by body weight to seven treatments (three replicates of 40 birds each treatment) including (1) basal diet (containing 0.04 mg of selenium (Se)/kg; control) without supplementary Se; (2, 3 and 4) basal diet + 0.05, 0.15 or 0.25 mg/kg Se as SS; (5, 6 and 7) basal diet + 0.05, 0.15 or 0.25 mg/kg Se as dl ‐Se‐Met. The experiment lasted 42 days. The results revealed that dietary Se supplementation improved (p < 0.05) average daily gain, feed efficiency, immune organ index, serum immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM) and triiodothyronine (T₃) concentrations and decreased (p < 0.01) thyroxine (T₄)/T₃ ratio in serum compared with the control. Broilers receiving the dl ‐Se‐Met‐supplemented diets had higher (p < 0.05) feed efficiency, thymus index, the amounts of IgA, IgG, IgM and T₃ as well as lower (p < 0.05) serum T₄ concentrations and T₄/T₃ ratio than those consuming the SS‐supplemented diets. Serum IgA and IgM levels of broilers fed 0.15 mg Se/kg were significantly higher (p < 0.05) than those of broilers fed 0.05 or 0.25 mg Se/kg. In summary, we concluded that dl ‐Se‐Met is more effective than SS in increasing immunity and promoting conversion of T₄ to T₃, thus providing an effective way to improve the growth performance of broilers. Besides, based on a consideration of all experiment indices, 0.15 mg Se/kg was suggested to be the optimal level of Se supplementation under the conditions of this study.     

Comparative pharmacokinetics of marbofloxacin in healthy and Mannheimia haemolytica infected calves

The pharmacokinetics of marbofloxacin were investigated in healthy (n=8) and Mannheimia haemolytica naturally infected (n=8) Simmental ruminant calves following intravenous (i.v.) and intramuscular (i.m.) administration of 2 mg kg(-1) body weight. The concentration of marbofloxacin in plasma was measured using high performance liquid chromatography with ultraviolet detection. Following i.v. administration of the drug, the elimination half-life (t(1/2 beta)) and mean residence time (MRT) were significantly longer in diseased calves (8.2h; 11.13 h) than in healthy ones (4.6 h; 6.1 h), respectively. The value of total body clearance (CL(B)) was larger in healthy calves (3 ml min(-1) kg(-1)) than in diseased ones (1.3 ml min(-1) kg(-1)). After single intramuscular (i.m.) administration of the drug, the elimination half-life, mean residence time (MRT) and maximum plasma concentration (C(max)) were higher in diseased calves (8.0, 12 h, 2.32 microg ml(-1)) than in healthy ones (4.7, 7.4 h, 1.4 microg ml(-1)), respectively. The plasma concentrations and AUC following administration of the drug by both routes were significantly higher in diseased calves than in healthy ones. Protein binding of Marbofloxacin was not significantly different in healthy and diseased calves. The mean value for MIC of marbofloxacin for M. haemolytica was 0.1+/-0.06 microg ml(-1). The C(max)/MIC and AUC(24)/MIC ratios were significantly higher in diseased calves (13.0-64.4 and 125-618 h) than in healthy calves (8-38.33 and 66.34-328 h). The obtained results for surrogate markers of antimicrobial activity (C(max)/MIC, AUC/MIC and T > or = MIC) indicate the excellent pharmacodynamic characteristics of the drug in diseased calves with M. haemolytica, which can be expected to optimize the clinical efficacy and minimize the development of resistance.     

CO2浓度增加对半干旱区马铃薯生长动态及产量、品质的影响

为了解CO₂浓度升高条件下马铃薯生长动态及其产量、品质的变化特征,在典型半干旱区（甘肃定西）利用开顶式气室(OTC)试验平台,以马铃薯‘新大坪’为供试品种,设对照(390 μmol·mol^-1)和590 μmol·mol^-1共2个CO₂浓度梯度,开展CO₂浓度增加模拟试验。结果表明：CO₂浓度增加至590 μmol·mol^-1时,马铃薯发育加快,生育期提前,全生育期天数缩短3 d;CO₂浓度升高对马铃薯株高、叶面积指数、叶绿素含量和叶片水势有明显促进作用。随着生育进程的推移,马铃薯地上部干物质生产对CO₂浓度的响应值呈现先变大再变小的趋势。CO₂浓度增加促进了马铃薯块茎产量的提高,单株结薯数和单株薯块质量也有所增加。与对照相比,590 μmol·mol^-1CO₂处理马铃薯块茎水分、蛋白质、维生素C含量以及铁、锌、铜元素含量分别下降3.43%、11.78%、13.09%、25.58%、31.94%和9.76%,而粗淀粉、粗脂肪、粗纤维和还原糖含量分别增加10.56%、240.00%、14.28%、106.38%。     

Evaluation of transpulmonary ultrasound dilution cardiac output in piglets: accuracy,precision and trending ability with room temperature injectate

ObjectiveTranspulmonary ultrasound dilution (TPUD) is a minimally invasive technique to measure cardiac output (CO) using a 1 mL kg^–1 isotonic 37 °C saline injectate indicator. The objective was to evaluate the performance of TPUD using a room temperature saline injectate.Study designProspective experimental trial.AnimalsA total of seven anesthetized male Yorkshire piglets.MethodsPiglets aged 1 month and weighing 7.7–9.0 kg were anesthetized with detomidine–ketamine–hydromorphone–isoflurane and a pulmonary artery flow probe (PAFP) placed via a median sternotomy. The thoracic cavity remained open during measurement of CO by PAFP and TPUD. The TPUD indicators of 1 mL kg^–1 0.9% saline at 37 °C and 20 °C were compared during infusions of phenylephrine and dobutamine, blood withdrawal and replacement. Bias, limits of agreement (LoAs) and percentage error (PE) between each iteration of PAFP and TPUD were measured with Bland–Altman plots. Trending ability via concordance, angular bias and radial LoA were compared.ResultsBland–Altman plots showed negligible bias with varying LoAs. PEs of 22% and 38% were found for 37 °C and 20 °C saline injectates, respectively. In the four-quadrant plots, the concordance rate was 94% and 100% for measurements obtained with 37 °C and 20 °C saline injectates, respectively. Angular bias for both were < ±5 °, with radial LoA < ±7 °.ConclusionsTPUD was accurate when using 1 mL kg^–1 of isotonic saline at 37 °C in a range of CO within 0.2–0.8 L minute^–1, and it reliably tracked positive and negative changes in CO. Room temperature (20 °C) indicator was less accurate but equally able to track direction of changes in CO.Clinical relevanceThe use of room temperature injectates allows an easy, readily available clinical application of TPUD CO monitoring while preserving the trending ability of the monitor.