草鱼SGLT1/2基因克隆及葡萄糖处理对其mRNA表达的影响

作为重要的转运载体,SGLTs(钠依赖性葡萄糖协同转运蛋白,sodium-glucose co-transporters)在物质的吸收过程中发挥着关键的作用.SGLT1和SGLT2作为SGLTs家族的重要成员,参与调节体内的葡萄糖吸收,从而维持血糖的稳态.为研究SGLT1和SGLT2在草鱼葡萄糖吸收过程中的作用,本实验...     

青海云杉移植苗不同施肥方法生长效益分析

通过不同施肥方法试验,研究有机肥、化肥及有机肥与化肥配施对青海云杉移植苗生长的影响。结果表明,施用化肥、有机肥均能促进青海云杉移植苗生长,而有机肥与N、P、K肥配施,才能获得更高的生长量。     

Preliminary Research on Regional Material Flow Analysis： A Case Study of Chengyang District in Qingdao

The method ＂Material Flow Analysis （MFA）＂ is one of the effective tools to study law and quantification of material flow between economic system and ecological system. On the national level, economy-wide material flow analysis has been published for a number of countries. However, published studies on the regional or local level are still very limited and a standardized method does not exist yet. On the basis of framework of Material Flow Analysis proposed by Eurostat, the paper collected related data and analyzed material input and output for the Chengyang district in Qingdao. The results showed that DMI （Direct Material Input） and TMR （Total Material Requirement） in absolute number increased about 3.6 and 3.9 times, respectively from 1995 to 2004. Fossil fuel and mineral contributed to about 50.3%-76.3% of DMI. Imports of material increased about 5.0 times and became the most important Contributor to DMI, which showed that local economic growth was highly dependent on resources from other regions and countries. DPO （Domestic Processed Output） and TDO （Total Domestic Output） represent slow increasing trend, and DPO contributed to 22.2%-58.1% of TDO, suggesting local hidden flows were of obvious effect on TDO. The biggest component of DPO is CO2, approximately accounting for 90% of DPO. The material productivity increased 57.7% in last decade, reflecting improvement of efficiency of resources utilization in some extent. However, compared to developed countries and regions, material productivity of Chengyang district was relatively low. Therefore, in order to promote the sustainability, it is essential to develop circular economy and enhance materials productivity.     

马尾松银松素合酶基因启动子区的克隆及特征分析

银松素合酶(pinosylvin synthase,PS)是合成银松素类植物抗毒素的关键酶。本文根据前期工作所获得的PS基因序列,设计基因特异引物,利用连接介导的染色体步行技术,从马尾松总基因组中扩增PS基因上游的启动子区;通过PLAN-CARE等分析软件对5′侧翼区近700 bp进行启动子特征分析,预测启动子区调控元件。结果表明,在翻译起始密码子上游167 nt处可能存在TATA-box。此外还发现3个GATA-box(-260 nt、-295 nt、-386 nt和-295 nt)和若干个TGAC-like序列。     

Worldwide rapeseed (Brassica napus L.) research: A bibliometric analysis during 2011–2021

Rapeseed(Brassica napus L.) is the world?s second produced oilseed and accounts for nearly 12% of world major vegetable oil production. For the last 10 years, the production, planting area, and yield of rapeseed have been stable, with improvement of seed quality and especially seed oil content. This paper presented the worldwide rapeseed research by using the bibliometric analysis of papers published during the period 2011–2021. In total,7617 articles and reviews were included in this analysis. ...     

夜蛾科昆虫区系研究Ⅱ.中国各省区夜蛾的分布及相似性分析

中国夜蛾共有20亚科845属3 751个有效种.这些种类有4种区系成分,其中东亚成分占优势,占总数的51.35%,东洋成分占25.51%,古北成分18.45%,广布成分仅2.27%.夜蛾在全国各省区的分布与区系结构各不相同,台湾、云南、西藏、四川、湖北、河南、湖南、浙江、新疆等是夜蛾物种丰富的省区.东亚成分在除东北、西北、华南以外的省区都是优势类群.多元相似性聚类分析结果显示,台湾和华中关系密切于华南;内蒙古和东北区的关系密切于西北;河南、湖北、陕西、甘肃、安徽、江苏6省聚为一个"秦岭淮河区",这个新分布区的特点是,东亚成分占优势;古北成分和东洋成分相等;古北东洋两界的分界线横贯全区.     

农业科技成果转化的制约因素与发展对策研究——以中国农业科学院某研究所为例

在国家提出加强创新驱动战略大背景下,农业科技创新与成果转化是夯实农业基础和战略地位的两大基石。文章针对当前多数农业科研所重视科技创新淡化成果转化问题,以中国农业科学院某研究所为案例并参照成果转化先进所的宝贵经验,通过实地调研、座谈走访、填写问卷相结合方式,运用问卷星统计分析方法进行综合研究,得出当前农业科研所成果转化在制度政策、人才、平台与资金等五个方面的制约。最后,针对这些制约因素,提出了促进成果转化的5条对策建议,以加快农业科技成果转化,促进农业科技创新,从而促进乡村振兴战略加快实施。     

聚类分析在我国果树研究中的应用及问题分析

对近１０年来聚类分析在我国果树研究中的应用情况进行了简要总结。涉及十余种果树及系统聚类和模糊聚类两种聚类分析方法。文中对有关问题进行了分析和讨论。     

甲萘威原药高效液相色谱分析方法

采用带紫外检测器和ODS-C18色谱柱的高效液相色谱仪,在流动相甲醇 水=80 20(V/V)、检测波长278nm、流速1.0mL/min的条件下,对甲萘威含量进行定量分析。结果表明,甲萘威的标准偏差为0.39;变异系数为0.41%;平均回收率为99.64%;线性相关系数为0.9998。