竞争性RT-PCR检测铜对Ctr1 mRNA表达的影响

为了应用竞争 RT- PCR法检测铜对猪传代肾细胞 (PK15 )中特异性铜转运蛋白 Ctr1基因 m RNA表达水平的影响 ,经 2次克隆 ,将筛选出一段 4 0 0 bp的核苷酸序列 ,插入 RT- PCR扩增出的 5 30 bp Ctr1目的片段中 ,构建了插入突变型 Ctr1- c DNA竞争模板。再将竞争模板与各试验组 c DNA同时进行 PCR扩增。结果 ,猪肾 PK15细胞 Ctr1基因m RNA表达量在 7.8～ 31.2 μmol/ L Cu范围内随铜添加浓度的升高减少 ,当铜添加浓度达到 6 2 .5 μmol/ L 时 ,其表达量又有所回升 ,呈双相反应。     

高铜对雏鸭肝影响的病理学观察

将360只1日龄天府肉鸭随机分为6组，分别喂以对照日粮（Cu8mg／kg）和高铜日粮（I组：Cu100mg／kg；Ⅱ组：Cu200mg／kg；Ⅲ组：Cu400mg／kg；IV组：Cu600mg／kg；V组：Cu800mg／kg）6周，研究日粮铜添加水平对雏鸭肝及其相关生化指标的影响。结果显示，Ⅱ组、Ⅲ组、Ⅳ组、V组雏鸭肝出现不同程度的病理损伤，肝细胞颗粒变性、空泡变性。Ⅳ组、V组肝铜含量和血清铜含量均显著高于对照组（P〈0．01）。Ⅲ组、Ⅳ组和V组血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶活性显著升高（P〈0．01）。结果表明，日粮铜含量达400～800mg／kg即可引起肝的病理损伤。     

日粮铜对猪生长性能及血清GH、INS、IGF-Ⅰ、IGFBP_3水平的影响

选用 6 0头断乳“军牧 1号”白猪 ,分成 6组 ,每组 10头 ,分别饲以添加 0、10 0、15 0、2 0 0、2 5 0、30 0 mg/ kg铜 (硫酸铜 )的日粮 ,进行为期 80 d的饲养试验 ,定期称重、采血 ,观测铜对仔猪生长性能及血液 GH、INS、IGF- 、IGFBP3 的影响。结果表明 :基础日粮中添加 10 0～ 30 0 m g/ kg铜均能促进仔猪的生长 ,降低饲料消耗 ,其中以 2 5 0 m g/ kg铜和第40～ 6 0天最为显著 (P<0 .0 1)。高剂量铜能显著提高循环血液中 GH、INS、IGF- 、IGFBP3 的水平 ,其中以添加 2 5 0mg/ kg铜水平 ,于试验期第 2 0～ 40天最为明显 (P<0 .0 1)。高铜促生长作用显然是通过促进 GH、INS、IGF- 的合成和分泌而实现的。     

实验性皮肤角化不全症仔猪被毛及各组织中锌、铜含量测定

以钙：锌＝３３０：１与５１６：１的日粮饲喂健康断乳仔猪１２０天，复制了仔猪皮肤角化不全症。患病仔猪被毛中锌、铜含量呈逐渐下降趋势，锌含量极显著低于正常组（Ｐ＜０．０１），而铜含量则与正常组差异不显著（Ｐ＞０．０５）。患病仔猪肝、十二指肠、肠系脱淋巴结中锌含量极显著低于正常组（Ｐ＜０．０１），心、肾、脑中锌含量著低于正常组（Ｐ＜０．０５），脾、肺、左侧最后肋骨、背最长肌、胰中锌含量与正常组差异不显著（Ｐ＞０．０５），肝、背最长肌中铜含量极显著高于正常组（Ｐ＜０．０１），脾中铜含量显著高于正常用（ｐ＜０．０５），脑、心中铜含量极显著低于正常组（Ｐ＜０．０１），肾中铜含量显著低于正常用（Ｐ＜０．０５），其余组织中铜含量与正常组差异不显著（Ｐ＞０．０５）。本试验测定了正常仔猪和用化不全症仔猪各组织中锌、铜的含量，报告了角化不全症仔猪心、肺、肠系膜淋巴结中锌、钢含量及被毛中锌、钢含量的动态变化。     

太原市儿童血清铜、铁、锌与血红蛋白的测定及其相关分析

作者经对太原市北城区和北郊区两个幼儿园118名3～7周岁学龄前儿童进行血清中微量元素钢、铁、锌和血紅蛋白水平测定,并进行相关分析。结果表明:学龄前儿童低锌和低铁与血紅蛋白的关系及低锌与低铁的关系,经单侧相关分析,得出均呈正相关关系,低铁与低锌呈—致性降低;而低铁与低铜无相关关系。     

日粮硒、铜水平对大鼠体内有关的抗氧化物酶活性及脂质过氧化产物的影响

本试验以大鼠为动物模型研究口粮中微量元素硒、铜对体组织中抗氧化酶活性及脂质过氧化物等的影响。试验按2×4随机设计,口粮含2个水平钢分别为0．54和6．05mg·kg-1,4个水平硒分别为0．021,0．301,0．605和1．102mg·kg-1。结果表明：组织GSH－Px活性随组织硒水平的升高而显著上升（P＜0．01）,缺硒时组织GST－Px活性下降（P＞0．05）。缺铜时大鼠组织Cu,Zn－SOD、血浆铜蓝蛋白活性极显著地下降（P<0.01）和肝脏等组织中GSH－PX活性也显著下降（P<0．05）。组织中脂质过氧化产物MDA丙二醛在硒和铜缺乏时显著上升（P＜0．05）。肝中GSH－Px活性不仅受硒水平影响,缺铜也下降了。试验认为：硒、银通过影响有关酶的活性而影响到机体的抗氧化能力。     

Remediation of a sandy soil artificially contaminated with copper using a polyacrylate polymer

Abstract. We investigated whether a Na-K polyacrylate polymer could be used to remediate a sandy soil artificially contaminated with copper. An experiment, carried out in solution culture, showed that ionic copper was rapidly trapped within the polymer to a maximum content of c. 190mg Cu g ^-1 dry polymer, the proportion needed for chelation of each copper ion by four carboxylic groups present in the polymer chains. Cu-EDTA was not retained by the polymer. Growth of perennial rygrass in 10 kg pots was stimulated in the gel-amended soil, and even in the pots with the highest levels of copper, growth was much less impaired than in pots without polymer. Copper concentrations of the shoot.; were smaller in the plants cultivated in the amended soil. Water extractable copper was considerably reduced in the contaminated gel-amended soil and polymer particles removed from the soil were shown to contain high levels of copper.     

AF-7500原子荧光光度计测定汞检出限的不确定度评估

在橡胶树割面涂施不同浓度硫酸铜，分析其对胶乳产量及生理参数的影响。结果表明：割面涂施硫酸铜对干胶含量、无机磷含量无明显影响，但在一定程度上提高了胶乳的蔗糖含量、硫醇含量和蔗糖转化酶活性，降低镁离子含量和黄色体破裂指数，提高橡胶树的干胶产量。     

Influence of Mineral Nutrients on Strawberry Fruit Quality and Their Accumulation in Plant Organs

Abstract

This review is based partly on complete articles and partly on abstracts. Three of the 60 articles deal with the total uptake of elements in strawberry plant organs in two different strawberry production systems, both considered as optimal concerning amount and balance of elements. The effect on fruit quality may be dramatic if the level of a particular element is outside this range, but there may also be effects initiated by differences within the optimal range of elements. Most articles refer to product oriented quality, but some focus on consumer oriented quality, as discussed by Shewfelt (1999). The discussion here is on a general basis, so one should keep in mind that there are cultivar differences and that specification of nutrition ideally should mirror the needs of a single cultivar, or a group of cultivars with similar requirements. Also, to get a complete understanding of the subject future reviews should embrace a broader access of information including the effect on plant development of individual elements, such as the role of calcium in fruit firmness and its importance in cell wall structure. However, the intention here is to narrow the information to results that suggest a direct connection between nutrient uptake and fruit quality.     

Examination of a practical method for copper enrichment of euryhaline rotifers (Brachionus plicatilis) as diet of Eriocheir sinensis zoea larvae

In this study, an effective method to enrich the rotifer Brachionus plicatilis with copper was developed as a feed for the Chinese mitten crab Eriocheir sinensis zoea larvae. Changes in the concentrations of other minerals in rotifers were also examined when copper was added for rotifer enrichment. The ability of Chlorella to absorb waterborne copper is much higher than that of rotifers, and hence, copper was preaccumulated in Chlorella before its ingestion by rotifers. The copper content in rotifers was comparable to the dietary copper requirement of the crab larvae when the rotifers were enriched with 0.1 mg Cu g⁻¹ Chlorella for 12 h. Further enrichment in rotifers with Cu‐enriched Chlorella and lipid emulsions did not significantly change the profile of major fatty acids and mineral composition in the rotifers. Evidence shows the feasibility of copper enrichment in rotifers using microalgae that can accumulate copper. This study indicates that copper in rotifers can be enriched by feeding copper‐enriched algae at a concentration of 0.1–0.2 mg Cu g⁻¹ Chlorella. The developmental rates of E. sinensis can be improved by feeding zoea larvae with copper‐enriched rotifers, but survival rates were not affected by dietary copper enrichment.