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81.
为研究不同发育时期橡胶树叶片的功能代谢及揭示天然橡胶胶乳的合成调控机制,本研究以巴西橡胶树古铜期幼嫩叶片(幼叶)和稳定期完全成熟叶片(成熟叶)为实验材料,提取蛋白并进行双向电泳分离,结果发现幼叶与成熟叶相比,蛋白表达图谱差异显著,鉴定出的已知蛋白中多数参与了碳代谢及蛋白的翻译后修饰功能。本研究建立了成熟的巴西橡胶树叶片比较蛋白质组学研究的技术体系,获得了高分辨率的叶片蛋白双向电泳参考图谱,鉴定出来的腈裂解酶A链,核酮糖1,5-二磷酸羧化酶和肌动蛋白等在幼叶和成熟叶片中的表达量不同,这些结果为进一步揭示天然橡胶合成调控机制提供了一定的技术支撑与实验数据,对天然橡胶合成机制的研究以及其它热带植物叶片的蛋白质组学研究具有一定的参考意义。  相似文献   
82.
Summary An aspect of cereal science that is becoming increasingly important is comparative genetics. Establishment of the relationship between genomes within polyploids, between species within tribes and between species within families will allow not only the integration of genetic maps but also the knowledge acquired of each of the species. Using a set of homoeologous probes, workers found the relationship between the three wheat genomes to be precisely collinear, after taking a few major translocation events into account. Transfer of the wheat map to rye led to the elucidation of similar relationships between the three wheat genomes and that of rye. Genome collinearity, however, extends even beyond tribes. In a comparison of the genomes of wheat, rice and maize, it was shown that despite the separation of these genomes for possibly 50 million years, gene order was still highly conserved. This collinearity between genomes can be exploited in a number of ways.  相似文献   
83.
云南省热带作物科学研究所高级系比试验19年的观察资料表明,橡胶树新品种云研73-477干胶产量高,早期产胶潜力大,生长快,生势整齐,抗寒能力强,割胶刺激反应为Ⅰ类刺激的优良新品种。可推荐在云南植胶区的轻、中寒害地区试验种植。  相似文献   
84.
焊工工艺学是培养学生全面掌握中级电焊工工艺技术理论知识的专业课程,其涵盖面广,学生学习吃力,教学难度较大。为使学生全面、彻底地掌握焊工工艺学课程的内容,运用比较教学法授课,收到较好的效果。  相似文献   
85.
    
Ovulation rate and prolificacy are the most important reproductive traits that have major impact on the efficiency of lamb meat production. Here, we compared the whole genomes of the Romanov sheep, known as one of the high prolific breeds, and four other sheep breeds namely Assaf, Awassi, Cambridge and British du cher, to identify genetic mechanisms underlying prolificacy in sheep. Selection signature analysis revealed 637 and 477 protein‐coding genes under positive selection from FST and nucleotide diversity (Pi) statistics, respectively. Further analysis showed that several candidate genes including LEPR, PDGFRL and KLF5 genes are involved in sheep prolificacy. The identified candidate genes in the selected regions are novel and provide new insights into the genetic mechanisms underlying prolificacy in sheep and can be useful in sheep breeding programmes to develop improved breeds for high reproductive efficiency.  相似文献   
86.
    
The productivity and financial performance of dairy farms in New Zealand and Japan (Hokkaido) were analyzed. By recomposing the official statistics on the dairy industry relative to Japan, New Zealand has low milk yield productivity per cow, but higher per hectare performance because of their seasonal breeding and pasture grazing systems. In the revenue and expenses analysis, New Zealand had a high cash surplus ratio compared with Hokkaido. In the cost analysis, for expenses on a “per kg of raw milk” basis, New Zealand milk production costs are 29% of those in Hokkaido. More than 60% of the production costs were attributable to feed, interest charges and wages in New Zealand. In Hokkaido, the feed cost alone accounted for 73% of the total cost. There is also a remarkably high interest expenditure in New Zealand caused by non‐subsidized fund procurement from finance organizations. In the financial analysis, New Zealand dairy farms have only approximately 50% of the total assets per cow compared with farms in Hokkaido. But total liabilities per cow in Hokkaido are twofold to threefold greater than in New Zealand. The difference between total assets and total liabilities shows that Hokkaido dairy farmers have an equity that is 50–200% greater than New Zealand dairy farmers. In the management analysis, New Zealand has a low turnover ratio of gross assets caused by the seasonal breeding system. In conclusion, New Zealand has higher per hectare production performance than Hokkaido, but has a low utilization of gross assets caused by a seasonal breeding system based on pasture grazing.  相似文献   
87.
    
Systemic mastocytosis is a rare phenomenon, with limited information regarding prognostic features and effective treatment of canine patients with this disease. The objective of this study is to determine the impact of certain features and treatments on dogs with systemic mastocytosis. The medical records of 40 dogs from 4 northeastern US veterinary hospitals, with evidence of systemic mast cell disease, were evaluated retrospectively. Variables analysed with relation to overall survival and prognostic significance included treatment protocol used, substage, presence of a cutaneous or visceral tumour, presence of multiple cutaneous Mast cell tumours, grade of the primary tumour and metastatic site(s). Dogs with metastatic disease confined to distant lymph nodes lived longer than those with circulating mast cells in the blood (P = .001), and those with metastatic disease evident in more than 2 sites had a worse prognosis than those with disease in a single location (P = .005). Additionally, administration of chemotherapeutic agents led to increased survival over prednisone therapy alone (P = .008), with the combination of lomustine, vinblastine and prednisone prolonging survival over the tyrosine kinase inhibitor, toceranib phosphate (P = .002). Presence of mast cells in the blood and/or evidence of disease in more than 2 sites indicate widespread dissemination suggesting their use as negative prognostic features. Furthermore, a chemotherapy protocol including combination lomustine and vinblastine therapy may be more effective than toceranib phosphate for the treatment of dogs with disseminated mast cell disease. Overall, patients with systemic mastocytosis have a grave prognosis and more effective treatment options are needed.  相似文献   
88.
    
Identifying the action of natural selection from patterns of standing genetic variation has long been of interest to the population genetic community. Thanks to the availability of large single‐nucleotide polymorphism (SNP) data sets for many species and of high‐throughput SNP genotyping methods, whole‐genomic surveys to detect selective sweeps are now possible. Knowing the ancestral allele increases the power to detect selection. We present here a comparative genomic approach to determine the putative ancestral allele of bovine SNPs deposited in public databases. We analysed 19 551 488 SNPs and identified the putative ancestral allele for 14 339 107 SNPs. Our predicted ancestral alleles were in agreement with ancestral alleles detected by genotyping outgroup species for 97% SNPs from the BovineSNP50 BeadChip. This comparison indicates that our comparative genomic‐based approach to identify putative ancestral alleles is reliable.  相似文献   
89.
于2013年进行13个杂交稻品种的品比试验,考察各品种综合表现,结果表明:适宜邵武市作烟后稻种植的主导品种为天优华占、谷优527、闽丰优3301、甬优9号,可推广种植;后备品种为广两优1128,可扩大试种;在稻瘟病轻发区可推广种植米质相对较优的Y两优1号、Y两优2号;在“翠碧一号”烟叶种植区推荐种植竹丰优3446.  相似文献   
90.
长吻鮠(Leiocassis longirostris Giinther)、大鳍鳠(Hemiba-grus macropterus Bleeker)、南方大口鲶(Silurus soldatorvi meridionalisChen).鲶鱼(Silurus asotus Linnaeus)的血清蛋白,经盘状电泳分离,从负极到正极可分为脂蛋白、球蛋白、运铁蛋白、清蛋白和前清蛋白5个组分。电泳图谱具种间差异,亲缘关系越远差异越大。同种鱼雌、雄间的电泳图谱基本相似,但不完全相同,鲶鱼具有雌性特异蛋白。  相似文献   
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