Recent advances in molecular genetics of forest trees

The use of molecular markers has greatly enhanced our understanding of the genome structure of forest trees. Conifers, in particular, have a relatively large genome, containing a very high proportion of repeated DNA, consisting of tandemly repetitive and dispersed repetitive DNA sequences. The nature of highly conserved tandemly repetitive rRNA genes has been investigated in a number of tree species, and their sites mapped on specific chromosomes by fluorescent in situ hybridization (FISH). Different families of retrotransposons (IFG, and TPE1) have been isolated and characterized from the dispersed repetitive DNA of pines. Genome maps have been constructed in a number of forest tree genera: Pinus, Picea, Pseudotsuga, Cryptomeria, Taxus, Populus, and Eucalyptus. EST databases have been established from cDNA clones of pines and poplars. The structure and maternal or paternal modes of inheritance of organelle genomes have been investigated in forest trees. Comparative mapping in conifers has shown that gene families are conserved across genera. Due to lack of polyploidy in conifers, the evolution of this group of trees may have occurred primarily by duplication and dispersal of genes, probably by retrotranspositions, to form complex gene families. The evolution of angiosperm tree species has presumably involved both gene duplication as well as genome duplication (polyploidy). Application of genetic engineering has shown that genes from phylogenetically unrelated organisms can be introduced and expressed in trees, thus offering prospects of genetic improvement of forest trees. This revised version was published online in August 2006 with corrections to the Cover Date.     

气力式水稻穴盘成型机气流分配室流场仿真与优化设计

合理的气流分配室结构是气力式水稻穴盘成型机正常工作的前提条件。为了保证气流分配室具有均匀的流场结构,生产出合格的穴盘,以气体控制方程为理论依据,利用FLUENT软件对气流分配室内气流场进行了仿真分析。结合正交试验设计和数值模拟技术,以腔体厚度、通气孔直径和底角为影响因素,速度不均匀系数为评价指标,对气流场进行了单因素和正交模拟仿真试验,结果表明各因素对试验结果影响的主次顺序为配气腔体厚度、通气孔直径、底角;当腔体厚度、通气孔直径和底角分别为110 mm、15 mm和0°时,气流分配室流场均匀性较理想,此时速度不均匀系数为11.37%。试验结果表明,仿真结果与实测结果的平均相对误差为1.59%,双侧相关系数为0.028,存在显著相关关系;穴盘生产成型率为92.3%,穴孔质量和底面厚度的变异系数分别为10.2%和9.81%,满足穴盘生产要求。     

基于改进蚁群算法的植保无人机路径规划方法

为了规划出更加高效的植保无人机路径,提出一种基于改进蚁群算法的植保无人机路径规划方法,该方法适用于多个具有复杂多边形边界与内部障碍物的三维作业区域。采用扫描方式生成水平面内的作业路径,经过离散化处理后,在三维地形曲面上插值,获得三维作业路径。在此基础上,建立作业路径生成算法,以三维作业路径总长度尽量短、作业路径数量尽量少为目标,对植保无人机作业航向进行寻优。改进蚁群算法通过附加记录作业路径进入点的机制,实现对三维作业路径的合理排序,生成总长度较短的转移路径。经过算例检验,针对同一作业区域规划出的三维作业路径与水平面内的作业路径的航向角存在较大差异,相差最大为92°,这说明考虑三维地形的必要性。算例中,将改进的蚁群算法与贪婪算法进行了对比,针对一系列相同的作业起点,改进的蚁群算法所得的转移路径总长度均较短,比贪婪算法所得结果缩短3%~28%;在未选定作业起点情况下,改进的蚁群算法与贪婪算法求得的转移路径总长度最小值分别为1661m与1763m,说明改进的蚁群算法具有良好的寻优能力。实例检验情况与算例所得结论基本一致。算例与实例中的作业区域边界与地形复杂,涵盖情况全面,表明本文提出的路径规划方法具有一定实用性。     

玉米空间分层施肥装置结构优化与试验

针对玉米分层施肥作业中开沟宽度大、回土效果差导致的肥料分层效果不明显、各层肥量难以控制等问题,设计了一种各层肥量可调的空间分层施肥装置,肥料可在土壤中形成半包围种子的分布状态。通过理论分析和设计计算确定了空间分层施肥装置的基本结构参数,明确了影响分层施肥装置内肥料颗粒运动的主要因素。运用离散元法对分层施肥装置工作过程进行仿真分析,选取施肥调节片前端宽度、后端宽度和安装角为试验因素,以上层和中层排肥口出肥量为试验指标,进行二次正交旋转组合仿真试验,建立了试验指标与影响因素的回归模型,仿真结果表明,当施肥调节片前端宽度为3.61mm,后端宽度为21.52mm,安装角为43.23°时,上、中、下3层排肥口施肥量比例为最佳施肥比例3∶3∶4。为验证仿真分析结果,在不同作业速度和不同施肥量条件下,进行了空间分层施肥装置的样机性能试验,试验结果表明,空间分层施肥装置能够实现各层肥量的目标施肥配比,在不同作业速度和不同施肥量下各层施肥量变异系数不大于4.3%,各层肥料深度误差在10mm以内,各层肥料横向距离误差在6mm以内,工作性能稳定。     

基于响应面和遗传算法的尾座式无人机结构参数优化

设计了一种垂直起降尾座式无人机,利用中心组合试验(Central composite design,CCD)对无人机的翼展长、后掠角、小翼高和小翼厚4个结构参数进行设计,构建了25组样本点。利用ANSYS CFX进行升阻比和阻力数值模拟,通过Design-Expert软件建立无人机结构参数与升阻比、阻力的响应面模型,其中升阻比随着翼展长和小翼高的增加而增大,后掠角和小翼高对升阻比的影响较小,当攻角为4°～8°时,升阻比随小翼厚的增加而减小,当攻角为10°～12°时,升阻比随小翼厚的增加而增大;阻力随着翼展长和小翼厚的增加而增大,随小翼高的增加而减小,随后掠角的增加先增大后减小。以升阻比最大和阻力最小为优化目标,采用多目标遗传算法进行结构参数优化,得到最优结构参数为:翼展长1 123 mm、后掠角34°、小翼高39 mm、小翼厚3 mm,与原始样机相比升阻比提高了12. 4%,阻力降低了5. 3%。采用风洞试验对响应面模型进行了验证,其中升阻比和阻力的数值模拟相对误差小于8. 0%,响应面模型相对误差小于3%,表明响应面模型具有较高的精度和良好的通用性,可用于垂直起降尾座式无人机的优化设计。     

3-RRPaR并联机构刚体动力学建模与分析

针对一种具有冗余结构的3-RRPaR并联机构,根据拉格朗日乘子法建立动力学模型,进而对冗余并联机构进行动力学分析。首先,分析了3-RRPaR并联机构的结构特征,确定了冗余结构的结构构型;然后,利用闭环矢量法建立并联机构的逆运动学模型,利用拉格朗日乘子法建立并联机构的动力学模型;最后,利用Matlab分别对并联机构空载和加载时的动力学方程进行数值求解,绘制了动力学响应曲线,根据拉格朗日乘子与约束力矩的关系求解出驱动杆的驱动力矩,将计算结果与ADAMS虚拟仿真结果进行对比分析,验证了动力学建模方法的正确性。结果表明,动平台加载后,驱动杆的驱动力矩也随之增大,但其数值变化规律与空载时基本相同。本研究为该并联机构的动力学控制和机构性能研究奠定了基础,也为其他结构冗余并联机构的刚体动力学建模提供了方法和思路。     

基于YOLO v3与图结构模型的群养猪只头尾辨别方法

在利用视频监控技术对群养猪只进行自动行为监测时,对猪只准确定位并辨别其头尾位置对提高监测水平至关重要,基于此提出一种基于YOLO v3(You only look once v3)模型与图结构模型(Pictorial structure models)的猪只头尾辨别方法。首先,利用基于深度卷积神经网络的YOLO v3目标检测模型,训练猪只整体及其头部和尾部3类目标的检测器,从而在输入图像中获得猪只整体及头尾部所有的检测结果;然后,引入图结构模型,描述猪只的头尾结构特征,对每个猪只整体检测矩形框内的头尾部位组合计算匹配得分,选择最优的部位组合方式;对部分部位漏检的情况,采取阈值分割与前景椭圆拟合的方法,根据椭圆长轴推理出缺失部位。在实际猪场环境下,通过俯拍获得猪舍监控视频,建立了图像数据集,并进行了检测实验。实验结果表明,与直接利用YOLO v3模型相比,本文方法对头尾定位的精确率和召回率均有一定提高。本文方法对猪只头尾辨别精确率达到96.22%,与其他方法相比具有明显优势。     

Effects of AMF inoculation on growth of Panax ginseng C.A. Meyer seedlings and on soil structures in mycorrhizosphere

We aimed to investigate the effects of inoculating Panax ginseng C.A. Meyer seedlings with arbuscular mycorrhizal fungi (AMF) by examining the root colonization, plant nutrition uptake, growth characteristics, and soil aggregation of P. ginseng seedlings inoculated at the time of transplantation. At 16 weeks, the AMF spore density per 30 g of fresh mycorrhizosphere in seedlings inoculated with AMF (AMF+ seedlings) was 256.8 and that in seedlings not inoculated with AMF (AMF− seedlings) was 186.3, respectively. The colonization rate of AMF in the lateral roots of AMF+ seedlings was approximately 19% higher than that in the lateral roots of AMF− seedlings. The patterns of AMF colonization in ginseng roots were similar to those of the Paris-type mycorrhizal association. Plant growth characteristics, such as plant height, root length, leaf area, number of lateral roots, fresh weight of shoots and roots, and chlorophyll content, were significantly enhanced in AMF+ seedlings compared to AMF− seedlings. The macronutrient content (P, K, and Ca) and micronutrient content (Cu, Fe, and Zn) of both shoots and roots were also significantly higher in AMF+ seedlings compared to AMF− seedlings. Furthermore, glomalin content and soil aggregation were significantly enhanced in AMF inoculated areas. Our results indicate that AMF inoculation may enhance the growth of ginseng seedlings by improving the uptake of mineral nutrients and the soil structure in mycorrhizosphere.     

饲料型四倍体刺槐组培苗的解剖学鉴定

利用石蜡切片法,研究NAA和IBA对饲料型四倍体刺槐组培苗营养器官组织结构的影响,发现:除茎以外,组培苗根和叶的解剖结构均出现了一定程度的差异,这些差异说明经NAA处理后的组培苗具有更强的输水、保水和抗逆能力,保持了饲料型四倍体刺槐抗旱性的特点.     

联合收割机割台总成的虚拟装配

研究了基于虚拟现实技术的联合收割机割台总成的虚拟装配.根据产品的装配结构模型,生成了联合收割机割台总成的装配结构树;采用UG建立了割台总成零部件的三维数字模型,导入到数字制造仿真软件,建立了总成各零部件的约束及装配关系,对割台总成进行了虚拟装配.