黄藤材的真菌变色

黄藤(Daemonorops margaritae),是我国热带和南亚热带森林中的主要伴生植物,是我国的优良商品棕榈藤种,为中国特有种(许煌灿等,1994a)。天然分布以海南岛为中心,延伸至23°30'N以南的广     

Equine hospital data as a source for study of prevalence and heritability of osteochondrosis and palmar/plantar osseous fragments of Swedish Warmblood horses

Reasons for performing study: Disturbances in skeletal development, primarily osteochondrosis (OC) and palmar/plantar osseous fragments (POF), have been commonly reported as problems in young horses. However, there are few reports of such findings for epidemiological analyses or breeding purposes. Objectives: To evaluate equine hospital data as a possible source of information for genetic evaluations by estimating prevalence and heritability of OC in the stifle, hock and fetlock joints and of POF in the fetlock. Methods: Data on Swedish Warmblood (SWB) horses were obtained from a large equine hospital in south Sweden. Prevalences were based on radiographic examinations of 879 screened horses, mainly evaluated as part of a prepurchase examination and 3639 horses with a reported history of orthopaedic problems. For the heritability study the 2 data sources were pooled and 3199 examined horses with pedigree information were considered for the linear animal model analyses. Results: The overall prevalence of OC was 13% (stifle 9%, hock 6% and dorsal osseous fragments in fetlock [DOF] 10%) and POF 10%. The overall heritability of OC was 0.05 on the visible binomial scale. The corresponding heritabilities for OC in the stifle were 0.03, hock 0.08, DOF 0.10 and POF 0.13. These values correspond to heritabilities of 0.09–0.38 on the underlying quantitative scale. Conclusions and potential relevance: Obtained prevalences and heritabilities were in accordance with other studies, supporting the hypothesis that data regularly obtained from equine hospitals may be a valuable source in studies of inherited disorders such as OC and POF. There is a need for more standardised documentation of diagnoses and consistent recording of identity of examined horses using passports or breed databases. Compilation of results from major clinics is desired in order to cover most progenies of stallions used in a region or nation.     

茎基腐亚洲镰孢菌侵染抗感小麦品种的组织病理学观察

小麦茎基腐是由多种镰孢菌侵染的世界性土传病害,亚洲镰孢菌(Fusarium asiaticum)是我国冬小麦主产区茎基腐镰孢菌的优势种群,对小麦生产造成巨大损失。本研究利用绿色荧光蛋白报告基因标记亚洲镰孢菌,研究其侵染抗感小麦的病理组织学过程,建立了茎基腐病菌与寄主互作的直观性的研究体系,对病害防治及抗病育种具有重要意义。基于PEG-CaCl_2介导原生质体转化法将gfp导入亚洲镰孢菌株CF0915,对转化子进行荧光表达、PCR验证、遗传稳定性、生长特性及致病力分析,选取与野生型表现相近的转化子进行侵染分析。结果表明,绿色荧光蛋白基因(gfp)与潮霉素基因(hyg)PCR扩增表明gfp已整合入真菌基因组中,转化子菌丝与分生孢子表现强烈绿色荧光信号,gfp能够在转化子中稳定遗传,菌落形态、生长速度及致病力与野生型菌株无显著差异;将gfp标记病菌分生孢子接种感病品种1 d后,大量孢子附着于根毛及根表皮细胞开始萌发,接种2 d后观察到抗性品种分生孢子萌发;感病品种接种3 d后,菌丝直接侵入表皮细胞或沿表皮细胞间层定殖生长,扩展至皮层组织,8 d后菌丝从根部迅速扩展至茎基部,至第10 d大量菌丝充塞根皮层细胞,叶鞘维管束也被菌丝侵染,并产生大量大型分生孢子,植株表现褐色病斑,14 d后根部及茎维管束被大量菌丝体填充,而后产生大量厚垣孢子,至25 d大部分感病品种幼苗萎蔫死亡;与感病品种相比,抗性品种在整个侵染过程中表现时间滞后。本研究对引起茎基腐病的亚洲镰孢菌侵染小麦的组织学过程观察,为病菌致病机理的阐释及抗病资源的利用提供了重要理论依据。     

浅谈桥梁变形成因及观测方法

桥梁变形的原因有:自然条件及其变化、与桥梁本身相联的原因、人为设计及施工不合理.观测方法:大地测量方法、特殊的测量方法、摄影测量方法.     

永定河下游沙地风沙活动典型分析

本研究以永定河河滩流动沙地为对象,通过对永定河河滩流动沙地及周边稀疏草地的风沙流活动、粗糙度、沙丘运移等的定位与半定位试验观测,对永定河河滩流动沙地的风沙流活动规律进行研究,并对该地区风沙流活动的影响因子进行了分析。结果表明,河滩地下垫面风沙流特征存在显著的差异,下垫面覆盖物不同时地表粗糙度也不同。     

Coronaviridae, pathogenetic and clinical aspects: An update

A review is given about pathogenetic and clinical aspects of the well-known as well as of recently detected members of the family Coronaviridae. Special attention is paid to coronavirus infections of domestic cattle and pets, whereas avian, murine, rat and human coronaviruses are summarized briefly.     

PP_(333)对温室葡萄枝条次生木质部细胞解剖结构的影响

为了研究设施栽培条件下葡萄木质部细胞的特征性结构及其受PP_(333)的影响,试验通过对温室葡萄喷施PP_(333)后的枝条次生木质部细胞的解离、观察,经统计分析,结果表明:(1)不同浓度PP333处理对各类型细胞的X形态结构没有明显影响;(2)浓度不同对各类细胞的长度和直径抑制作用不同,喷施1500mg/L浓度的PP_(333)对其营养生长的抑制效果最好。通过该试验的研究,为更好地探索设施栽培条件下果树的生理生态特征和化学调控技术提供了依据。     

特种用途皂荚优良无性系选择研究

为解决皂荚生产上存在的没有工业原料专用良种的问题,在枣庄、邹城、临沂等地现存的3万余株20~300年生皂荚大树中,筛选出优良单株4株,分别在山亭区、峄城区、滕州市、市中区等地进行生长量、产量、适应性、稳定性比较试验。综合分析,选择出工业原料用羊肠子、多刺速生、长荚、早丰等4个优良无性系,其产籽量超过对照48.3%~142.5%,其它指标优于对照。     

黑松引种试验研究

对引种的黑松进行了育苗和多点造林试验,从生长和抗逆性上进行了观测.苗期试验表明,黑松苗期在小陇山林区适应性强,苗期保存率高于油松,苗期生长表现与油松差异不显著,2年生苗木平均地径0.61 cm ,平均苗高16.30 cm.多点造林试验表明,黑松适应小陇山林区的气候、立地条件,抗鼠害能力比油松强,造林成活率和保存率比油松高,幼林生长表现与油松差异不显著.8年生黑松林分平均胸径3.20 cm,平均高2.98 m.引种获得成功,丰富了小陇山林区营造生态公益林的种质资源.