我国蔬菜清洗技术的研究现状与展望

蔬菜清洗是蔬菜加工过程中的重要的环节,它直接影响蔬菜加工的质量。随着人们生活水平的提高,蔬菜需求量不断增加,推动了蔬菜清洗技术的发展。探讨了蔬菜加工中应用的各种清洗技术,简要阐述了我国蔬菜清洗技术的未来发展方向。     

Evaluation of fertilizing capacity of cryopreserved rainbow trout sperm

ABSTRACT:   Experimental insemination was performed using artificially produced low-motility sperm. A mathematical model was applied to the results of the insemination in order to clarify the relationship between sperm motility, the density of sperm and the fertilization rate of eggs. In the model, the probability of fertilization by individual spermatozoa was a function of sperm density in the insemination solution. The results showed that the probability of fertilization clearly decreased with increased sperm density, and the maximum possible fertilizing rate by increasing the sperm density was constrained by the proportion of motile sperm (% motility). The model was also applied to the results of insemination tests of cryopreserved sperm in order to evaluate the fertilizing capacity of cryopreserved sperm. It was proven that cryopreserved sperm needed a higher density to obtain the maximum fertilization rate compared with fresh sperm, and it was anticipated that the ratio of the motile inseminated cryopreserved sperm should be more than 5.0% to achieve an egg fertilization rate greater than 90%.     

宽行窄幅错位密播种植方式对夏玉米光合特性及产量的影响

以登海618为试材,常规等行距种植方式为对照,设置不同种植密度、宽窄行种植、行间错位,比较研究宽行窄幅错位密播种植方式对夏玉米光合特性及产量的影响。结果表明,宽行窄幅错位密播种植方式下夏玉米叶面积指数和群体干物质积累量随着种植密度的增加而增加,叶片净光合速率逐渐降低;与对照相比,密度为86 625、101 055株/hm~2时叶片后期衰老缓慢,维持较高净光合速率的时间较长,花后群体干物质积累较多,各器官干物质运转率和对子粒贡献率较高。Richards模型解析表明,宽行窄幅错位密播种植方式下密度为86 625、101 055株/hm~2时达最大灌浆速率的时间较早,起始势和最大灌浆速率较高,子粒灌浆活跃期和灌浆速率最大时的生长量均偏低,植株空秆率较低,分别比对照显著增产5.61%、12.29%。密度为101 055株/hm~2时穗长、穗粗、行粒数、百粒重、穗粒重均比对照偏低,较高的有效收获穗数足以弥补劣势,使子粒获得高产。     

Alteration in important quality traits and antioxidant activities in Brassica oleracea with Ogura cybrid cytoplasm

In vegetable brassicas, cytoplasmic male sterility (CMS) system is the most preferred mechanism for hybrid seed production and Ogura cybrid cytoplasm is the only source used widely. Effects of the alien cybrid cytoplasm on important quality traits need to be understood for their effective use in breeding programme. In analysing 38 Ogura cybrid cytoplasm‐based cauliflower CMS lines, it was revealed that introgression of Ogura cytoplasm resulted in significant alteration in important quality traits. Cupric reducing antioxidant activity and ferric reducing ability of plasma values were increased up to 5–10 times in different genotypes. Among 38 analysed CMS lines, concentration of plant pigments such as anthocyanin, total chlorophylls and ascorbic acid was reduced in 21, 17 and 32 genotypes, respectively. However, the concentration of total carotenoids and β‐carotene was elevated in most of the CMS lines and total carotenoid was increased up to 20 times in the CMS line, Ogu13‐85‐2A. The results indicate the role of nuclear–cytoplasmic interaction and mitochondrial genome in determining concentration of different quality traits.     

Sorghum extrusion process combined with biofortified sweet potato contributed for high iron bioavailability in Wistar rats

This study aimed to evaluate the effect of sorghum and sweet potato on the bioavailability of iron, gene expression of proteins involved in iron metabolism and the plasma antioxidant capacity in animals fed with whole sorghum grains processed by dry heat or extrusion, combined or not with sweet potato flour with high content of carotenoids. Five experimental groups were tested (n = 7): dry heat sorghum flour (DS); extruded sorghum flour (ES); whole sorghum flour + sweet potato flour (DS + SP); extruded sorghum flour + sweet potato flour (ES + SP) and positive control (FS). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency, gene expression of divalente metal transporter 1 (DMT-1), duodenal citochroma B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The ES + SP group showed higher (p < 0.05) expression of DcytB, ferroportin and hephaestin when compared to the control group. The DS group showed high (p < 0.05) expression of DMT-1 and the ES showed high mRNA expression of transferrin and ferritin. The changes in the sorghum physicochemical properties from extrusion process reduced the iron and phytate content, and increased the gene expression of proteins involved in iron metabolism, improving iron bioavailability. The combination of sweet potato and sorghum flour (dry or extruded) improved the iron capture and total antioxidant capacity, probably due to the presence of β-carotene and antioxidant compounds.     

Combined mutations in five wheat STARCH BRANCHING ENZYME II genes improve resistant starch but affect grain yield and bread-making quality

Increases in the proportion of amylose in the starch of wheat grains result in higher levels of resistant starch, a fermentable dietary fiber associated with human health benefits. The objective of this study was to assess the effect of combined mutations in five STARCH BRANCHING ENZYME II (SBEII) genes on starch composition, grain yield and bread-making quality in two hexaploid wheat varieties. Significantly higher amylose (∼60%) and resistant starch content (10-fold) was detected in the SBEII mutants than in the wild-type controls. Mutant lines showed a significant decrease in total starch (6%), kernel weight (3%) and total grain yield (6%). Effects of the mutations in bread-making quality included increases in grain hardness, starch damage, water absorption and flour protein content; and reductions in flour extraction, farinograph development and stability times, starch viscosity, and loaf volume. Several traits showed significant interactions between genotypes, varieties, and environments, suggesting that some of the negative impacts of the combined SBEII mutations can be ameliorated by adequate selection of genetic background and growing location. The deployment of wheat varieties with increased resistant starch will likely require economic incentives to compensate growers and millers for the significant reductions detected in grain and flour yields.     

湿热地区毛细管地板供冷末端性能实测分析

选取重庆某住宅卧室进行实测分析,在供水温度为20、18、16℃,室外天气为晴天、多云、阴天（雨）工况下,研究毛细管地板供冷房间温度、地板供冷量、地板结露特征。供水温度为20、18、16℃时,室内最高温度27.6℃,最大供冷量分别为23、33、32 W/m²。开始结露时间为10：30-14：50之间,其中80%区域开始结露的时段为11：00-13：00之间,室内气流涡旋区最早出现结露。供水温度为18℃时,除湿能明显改变地板结露现象;供水温度为16℃时,除湿能改变大部分地板结露现象,室内气流涡旋区仍为结露区,但结露时间延后1 h以上。地板结露区域与室内气流组织有关,毛细管铺设位置应避开房间气流漩涡区。     

含水率和颗粒级配对稍密碎石土抗剪性能及地基承载力的影响

碎石土因特殊的工程特性而被科研人员关注,但对大粒径、粉质粘土填充、稍密状态下碎石土的力学性能涉足较少。根据该类型碎石土特点及大量勘察结果配置出两种比较典型级配的稍密碎石土,通过大型直剪试验仪测定了不同含水率状态下碎石土试样的抗剪强度,绘制剪应力与水平位移的关系图,通过一元回归得出稍密碎石的抗剪强度指标c、φ值,分析了不同含水率、不同级配对稍密碎石土抗剪性能的影响。结果表明：随着含水率的增大,碎石土粘聚力总体呈减小趋势,但变化趋势具有显著的阶段性且和粉质粘土的含量有关,含水率变化对碎石土内摩擦角的影响较小。用规范推荐方法计算了该类型碎石土地基承载力,并与地方规范经验取值和勘察工程实践取值进行了对比,结果表明：含水率对地基承载力影响较大,且与碎石土填充物的粉质粘土含量有关,粉质粘土含量越高,承载力影响越大。     

基于生态文明的半干旱区土地资源综合承载力评价研究

土地资源面积的有限性,位置固定性和区域差异性,造成了其供给稀缺性;而土地资源具有养育、支撑、资源和资产等功能,其需求旺盛;土地资源供给的稀缺性与社会需求的无限性之间存在矛盾。笔者运用经济学的总需求-总供给模型(AD-AS模型),立足生态文明角度,从供求方面对区域土地资源承载力进行探讨,以山西省大同市为案例区。研究结果表明:(1)由耕地需求量、建设用地需求量和生态用地需求量构成的土地资源综合承载力总需求,2011—2015年大同市土地综合承载力总需求分别为0.5309、0.5542、0.5770、0.5797和0.6030;其总体趋势增加;(2)由耕地支撑能力、建设用地支撑能力和生态支撑能力构成的土地综合承载力总供给,2011—2015年大同市土地综合承载力总供给分别为1.423、1.466、2.104、1.531和1.509;(3)2011—2015年大同市土地综合承载力处于超载,2013年超载最大,2015年超载最小,说明大同市的土地资源承载力超载现象在改善。运用此方法进行土地资源综合承载力评价,在土地资源承载力研究中更为科学和合理,使得人们更为关注生态文明,为建立社会、经济、生态和谐的人地关系提供了新的依据。     

耕作方式及秸秆还田对土壤性质、微生物碳源代谢及小麦产量的影响

以小麦品种济麦22为试验材料,采用裂区试验设计,耕作方式为主区,分别设常规翻耕(C)、深松(S)、旋耕(R)处理,副区为秸秆还田量,分别设秸秆全还田(P)和秸秆不还田(A)处理,采用Biolog Eco技术测定土壤微生物碳源代谢功能,并分析土壤基本理化性质和作物产量。结果显示:深松与秸秆还田均有利于土壤含水量和有机碳含量的提高,0～15 cm土层分别提高了9.78%和24.00%,15～30 cm土层分别提高了7.08%和15.81%;深松提高了15～30 cm土层的pH值6.67%,秸秆还田提高了0～15 cm土层的pH值4.32%。深松和秸秆还田均有利于代谢多样性(丰富度指数、香浓多样性指数)、碳源代谢强度的提高,0～15 cm土层分别提高了26.84%、3.84%和38.02%,15～30 cm土层分别提高了11.87%、 3.63%和14.74%。主成分分析表明常规翻耕秸秆不还田和旋耕耕作秸秆不还田碳源代谢功能相近,15～30 cm层次内常规翻耕秸秆全还田碳源代谢功能和深松耕作秸秆全还田处理相近。深松和秸秆还田平均提高了小麦产量5.82%,微生物碳源代谢功能与小麦产量具有极显著的相关性。