热处理对低温胁迫下黄瓜活性氧代谢和膜脂组分的影响

为探讨热处理减轻黄瓜果实低温冷害的作用机理,本试验研究了47℃热水浸泡5 min处理对黄瓜果实在4℃贮藏期间活性氧代谢和膜脂组分的影响。结果表明,47℃热水5 min处理可显著抑制黄瓜果实冷害的发生,贮藏15 d后果实冷害指数较对照(CK)低20.87%。此外,热处理还可抑制黄瓜相对电导率及丙二醛(MDA)含量的上升,提高黄瓜果实超氧化物歧化酶(SOD)、过氧化物酶(DOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性,抑制超氧阴离子($\mathop{{O}}_{2}^{{\mathop{}_{\ ·}^{-}}}$)及过氧化氢(H₂O₂)的产生,同时降低脂氧合酶的活性,保持较低的饱和脂肪酸含量、较高的不饱和脂肪酸含量和膜脂不饱和度。综上,热处理可维持黄瓜果实活性氧代谢的平衡,抑制膜脂的过氧化作用,从而提高黄瓜果实的抗冷性,减轻果实冷害损伤。     

高寒城市郊区外来园林树种适应性评价

为了确定外来园林树种在高寒城市郊区的适应性,以典型区域——青海西宁市火烧沟外来园林树种为对象,基于野外定株监测及室内统计分析,对其抗寒性、性状稳定性及生长发育能力等进行了研究,并综合评价了不同树种的适应能力。结果表明：（1）乔木树种紫叶稠李主干基部在晚冬期出现轻微冻裂,其他3种乔木在早春期发生了严重的抽条及轻度的冻害,4者叶色或叶形均明显改变,适应能力依次为：紫叶稠李>金叶复叶槭>金枝国槐>金叶榆。（2）小乔木类树种均发生了较严重的抽条,芽、叶、花出现了明显的冻斑,花朵变色或畸形率低于10%,适应性强弱依次为：西府海棠>独干红叶榆叶梅=绚丽海棠>红叶碧桃。（3）几种丁香属植物抵御冻害能力较强,仅部分物种一年生枝发生了轻微的抽条,但一些物种后期生长发育能力较差,例如黄丁香不发育花器官,综合适应能力大小为：紫罗兰丁香=波峰丁香=红叶丁香>辽东丁香>其他5者。（4）小灌木类树种胶东卫矛和小叶黄杨冬季地上部全部冻干,其他小灌木也发生了不同程度的冻害和抽条,球冠类适应能力为：榆叶梅=珍珠绣线菊>金叶榆>圆柏,绿篱类为：水蜡=紫叶矮樱>金...     

草鱼肝微粒体的提取及CYP酶活性的测定

CYP酶代谢是药物生物转化的主要途径,其数量和活性大小直接影响药物在体内的活化与代谢。我们对草鱼肝微粒体CYP酶含量及其活性进行了初步研究,以差速离心法提取草鱼肝微粒体,以CO还原差示光谱法测得CYP酶及细胞色素b5含量分别为0.619±0.102 nmol/mg、0.264±0.042 nmol/mg。以7-乙氧异吩噁唑酮-O-脱乙基反应、苯胺-4-羟化反应、氨基比林-N-脱甲基反应作为CYP1A、CYP2E、CYP3A的探针反应,测得EROD酶活为0.043±0.004 nmol/mg/min,ANH酶活为0.028±0.002 nmol/mg/min,AMND酶活为0.207±0.035 nmol/mg/m in。结果表明草鱼肝微粒体中CYP酶发育完好,并且具有参与药物代谢的3种主要亚型活性,其含量与活性大小与其它实验动物相差较大。本实验的方法与结果为草鱼CYP酶的系统研究提供可靠手段,最终为指导水产合理用药提供理论依据。     

Recording cerebellar neuron activities in swimming goldfish

ABSTRACT:   Neuronal activities were investigated in the cerebellum of immobilized and swimming goldfish Carassius auratus . Extracellularly recorded neural activities of the cerebellum in immobilized goldfish were characterized and classified into five types. Based on the waveforms and recording depths, these five neural activity types were estimated to originate from three identified classes of cerebellar neurons: Purkinje cells, eurydendroid cells, and granule cells. Chronic recording of cerebellar neuron activities in unrestrained goldfish was performed for more than 100 h. During the chronic recordings, a submersible amplifier attached to the goldfish head, and a multielectrode array developed for the present study were used. Neuronal activities in the cerebellum of free-swimming fish could also be classified into five types as in the immobilized condition. Firing patterns of two neurons identified as Purkinje cells and eurydendroid cells were analyzed during turning movements of the goldfish. The firing patterns of these neurons changed in relation to turning movements. Although some improvements are required, the chronic recording method developed in the present study can be applied to further investigations concerning the direct relationship between brain neural activities and certain behavior.     

Effect of dietary betaine levels on growth performance and hepatic intermediary metabolism of GIFT strain of Nile tilapia Oreochromis niloticus reared in freshwater

An 8‐week feeding experiment was conducted to determine the effect of dietary betaine levels on the growth performance and hepatic intermediary metabolism of genetically improved farmed tilapia (GIFT) strain of Nile tilapia Oreochromis niloticus (mean initial body weight: 78.3 ± 1.3 g, means ± SD). Six practical diets were formulated with the incorporation of betaine at the levels of 0 (control), 5, 10, 15, 20 and 25 g kg⁻¹. Survival showed no significant differences among the treatments (P > 0.05). The highest and lowest weight gain (WG) and specific growth rate (SGR) were observed for fish fed the diets containing 5 and 0 g kg⁻¹ (control) betaine, respectively. Feed intake showed similar trend with WG and SGR. In contrast, feed conversion ratio was the lowest when dietary betaine level was 5 g kg⁻¹. In general, dietary betaine supplementation showed no significant effect on hepatic composition of tilapia. Condition factor and viscerosomatic index tended to increase with increasing dietary betaine levels from 0 to 5 g kg⁻¹ and then decline when dietary betaine levels further increased from 5 to 25 g kg⁻¹. In contrast, hepatosomatic index declined with increasing dietary betaine levels (P < 0.05). Dietary betaine levels significantly influenced several hepatic enzymatic activities, including succinate dehydrogenase, lactate dehydrogenase, malic dehydrogenase, lipoprotein lipase and hepatic lipase, suggesting that dietary betaine addition had significant effects on nutrient metabolism in the liver. Based on the second‐order polynomial regression analysis of WG, 12.5 g kg⁻¹ of dietary betaine level seemed optimal for genetically improved farmed tilapia strain of O. niloticus.     

长春市主要绿化树种适应低温冻害能力的调查与分析

以长春市部分绿地的主要园林绿化树种为对象,对其适应低温冻害的能力进行了调查与分析,结果表明:在2009～2010年冬春季节持续低温作用下,12种外来树种中未遭受冻害的有青杄等7种,占58.3%;油松、丹东桧柏、大径级垂榆、京桃、刺槐普遍遭受冻害,都有死亡植株发生,其中大径级垂榆冻死率高达63.6%。低温累积效应是导致树木遭受冻害的主要因素,种间的遗传差异亦是因素之一。乡土野生树种无冻害相发生。     

Effects of dietary carbohydrate sources on the growth performance and hepatic carbohydrate metabolic enzyme activities of juvenile cobia (Rachycentron canadum Linnaeus.)

An 8-week feeding trial was conducted to evaluate the effect of dietary carbohydrate sources on the growth performance and hepatic carbohydrate metabolic enzyme activities of juvenile cobia. Six experimental diets were formulated to contain 20% glucose, sucrose, maltose, dextrin, corn starch and wheat starch respectively. The results indicated that fish fed the wheat starch and dextrin diets showed significantly better weight gain, specific growth rate and protein efficiency ratio compared with those fed the other diets. However, fish fed the glucose diet had a significantly lower survival and condition factor than those fed the other diets. There were significant differences in the total plasma glucose and triglyceride concentration in fish fed diets with different dietary carbohydrate sources. Haematocrit, haemoglobin, red blood cell and leucocytes were significantly affected by the dietary carbohydrate sources. The activities of glucose-6-phosphate dehydrogenase (G6PD), 6-phosphofructokinase (PFK) and fructose-1,6-bisphosphatase (FBPase) were significantly affected by the dietary carbohydrate sources, while fish fed the glucose diet showed higher G6PD, PFK and FBPase activities than those fed the other diets. These data indicated that dextrin and wheat starch were the most optimal carbohydrate sources for juvenile cobia.     

肥水措施对小麦穗部性状抗低温冷害的影响

研究了水肥调控下小麦拔节后低温冷害对其穗部性状的影响。结果表明,灌2水(越冬水、拔节水)和灌1水(越冬水)比不灌水平均穗粒数分别增加25.81%和19.89%;施用专用有机肥比不施平均穗粒数增加6.53%。     

苹果斯氏刺瘿螨发生与危害调查

斯氏刺瘿螨是四川省苹果上新发现的一种危害苹果叶片的害螨，以雌成螨在苹果芽的芽腋两侧越冬，翌年苹果芽萌动时开始出蛰。全年以５月中旬至６月中旬发生数量最多。气候温暖、低湿、少雨发生重，相反则轻。在调查的８个品种中，叶片多茸毛的品种受害重。天敌主要为捕食螨