八氢番茄红素合成酶(PSY2)基因果实特异表达载体的构建

八氢番茄红素合成酶是番茄红素合成的关键酶,通过PCR法获取PSY2基因和E8启动子序列,将目的基因和E8启动子序列构建到植物表达载体pBI101.2中,构建了果实特异表达启动子的八氢番茄红素合成酶基因植物表达载体。并采用PCR、限制性内切酶酶切和序列测定分析法,对重组质粒进行鉴定。结果表明,番茄果实特异性表达PSY2蛋白的重组质粒构建成功;通过农杆菌直接转化技术将其成功转入转化农杆菌LBA4404、EHA105,为下一步PSY2蛋白在番茄果实中特异表达奠定了基础。     

铜绿假单胞菌菌剂载体的筛选

为将铜绿假单胞菌应用于重金属污染环境的生物修复,以硅藻土(1~3 mm)、硅藻土(3~6 mm)、活性炭和轻石为材料,通过测定不同材料的吸水率、菌体吸附与释放作用及制备菌剂的活菌数,筛选出适宜的菌剂载体.结果表明,在设定的载体浓度下(0~20 g·L-1),不同载体的菌体去除率随浓度提高而增大,Q值在4.90%~49.60%之间;载体负载量随浓度提高而减小,L值在923.33~82.83 mg·g-1之间;硅藻土、活性炭的Q值在30 min达到最大.硅藻土(1~3 mm)、硅藻土(3~6mm)、活性炭和轻石的吸水率依次为60.3%、43.3%、23.4%和33.1%;以上4种载体制备的菌剂(菌剂A、B、C和D)的活菌释放率依次为27.4%、28.8%、19.7%和37.1%.室温保存30 d后,菌剂A活菌数为6.25×108CFU·g-1,减少了78.4%,菌剂B活菌数为1.12×1010CFU·g-1,是初始值的5.38倍,菌剂C的活菌数为2.95×108CFU·g-1,减少了73.9%,菌剂D的活菌数为3.61×109CFU·g-1,增殖了127.0%.可见,载体浓度及吸附时间显著影响其对菌体的吸附作用,3~6 mm硅藻土的负载量适中,菌体存活率高,活菌释放率高,可作为铜绿假单胞菌的菌剂载体.     

Characterisation of genes isolated from a potato swellingstolon cDNA library

Summary In screening to isolate a full-length copy of a previously isolated cDNA clone, a further three cDNAs were also isolated from a library prepared from sub-apical swelling-stolon tissue of potato (Solanum tuberosum L.). Sequence analysis showed these clones to be similar to extensin-like protein genes, acyl carrier protein thioesterase genes and high mobility group protein genes, respectively. A further cDNA, isolated by subtractive hybridisation, was similar to a tomato cDNA previously isolated on the basis of its down-regulation following nematode infection. While all the newly isolated genes were expressed in swelling stolons, for most, maximal expression was seen to be in stem tissue. Possible roles for these genes in the development of potato plants are discussed, as is the significance of gene expression in stems and stolons to the process of tuberisation.     

<Emphasis Type="Italic">Stenotrophomonas maltophilia</Emphasis>: a new potential biocontrol agent of <Emphasis Type="Italic">Ralstonia solanacearum</Emphasis>, causal agent of potato brown rot

Stenotrophomonas maltophilia was isolated from the rhizosphere of eggplant in the Nile Delta of Egypt, and its antagonistic potential against Ralstonia solanacearum race 3 biovar 2, the causal agent of potato brown rot, was in vitro evaluated on KB agar medium and in vivo on potato plants. In vitro, four isolates of S. maltophilia (PD3531, PD3532, PD3533, and PD3534) appeared antagonistic. The isolate (PD3533) was screened as the most promising antagonist for the in vivo tests. In the greenhouse, the antagonist was applied directly to soil or by bacterization of potato eyepieces. Stenotrophomonas maltophilia significantly suppressed potato brown rot in Egyptian clay soil but not in Dutch clay soil. Survival of a rifampicin and chloramphenicol-resistant S. maltophilia strain PD4560 was investigated in two pairs of clay soils, conventionally and organically managed, from Egypt and the Netherlands. The survival of S. maltophilia was significantly less in Dutch than in Egyptian soils, while the converse occurred for R. solanacearum. These results are in agreement with those obtained in the in vivo biocontrol tests. In conclusion, S. maltophilia may be useful for control of brown rot in the area where it was originally isolated, the Nile Delta in Egypt.     

一种改进的载波抑制调制光毫米波信号的产生方案

为了克服光纤无线(ROF)系统中色散对光载波抑制(OCS)调制光毫米波信号传输的影响,提出一种改进的OCS调制方案。使用双驱动马赫-曾德尔调制器(MZM),通过调整两路输入射频信号相位、基带信号增益和直流偏置电压将2.5Gbit/s数据信号仅调制到(OCS)信号的一个边带上传输。理论分析表明,与传统OCS调制光毫米波信号产生方案相比,本文方案解决了色度色散引起的码元走离问题,大大增加了传输距离。仿真实验结果表明,经过110km光纤传输后信号的眼图仍然十分清晰,在BER=10-10条件下,信号经过20、40和60km光纤传输后的功率代价分别为0.78、1.7和1.9dB。     

变压器隔离型DSTATCOM在不平衡系统中的补偿性能

【目的】针对配电网三相不平衡负载补偿问题,研究变压器隔离型配电网静止同步补偿器(DSTAT-COM)的补偿性能。【方法】以变压器隔离型逆变器为DSTATCOM的主电路,建立其数学模型,采用基于旋转坐标变换的方法计算DSTATCOM的补偿参考电流,提出瞬时无功功率分相控制与载波移相SPWM(Cps-SPWM)调制技术相结合的控制策略。在PSCAD/EMTDC环境中,对变压器隔离型DSTATCOM的补偿性能进行仿真研究。【结果】基于旋转坐标变换的补偿电流计算方法,能快速计算各相补偿电流,瞬时无功功率分相控制与Cps-SPWM调制技术相结合的控制策略,能有效地降低系统电流的不对称度,提高系统的功率因数,优化负荷电流的谐波性能。在各种负荷不平衡情况下,变压器隔离型DSTATCOM均具有良好的补偿性能。【结论】变压器隔离型DSTATCOM主电路结构简单,易于分相控制;瞬时无功功率分相控制与Cps-SPWM调制技术相结合的控制策略,能对不平衡负荷实现快速补偿,满足配电网对不平衡负荷的补偿要求。     

RTK技术解析及在工程测量中的应用

GPS（RTK）改变了传统的测量方式,使原本复杂、费时、费力的工作变得简单,它的出现是测绘行业发展的重大里程碑,对测绘行业的发展起到巨大的推动作用。     

膨胀石墨的多孔结构及其应用

膨胀石墨作为一种多孔材料，有望在溢油吸收、脱除废气、催化剂载体等多方面得到广泛应用。综述了有关膨胀石墨孔结构的研究结果以及它在有关领域的应用。     

Flavobacterium psychrophilum in rainbow trout, Oncorhynchus mykiss (Walbaum), hatcheries: studies on broodstock, eggs, fry and environment

The occurrence of Flavobacterium psychrophilum at four rainbow trout hatcheries was investigated to provide more knowledge about the reservoirs and transmission of this bacterium. Broodstock were sampled at stripping (including both unfertilized and fertilized eggs), and the offspring were then sampled at the eyed egg and fry stages. Water and surface samples (e.g. hatchery trays) were also sampled. Flavobacterium psychrophilum was found in ovarian fluid and milt, indicating that broodstock may serve as a reservoir and are latent carriers of the pathogen. Flavobacterium psychrophilum was not found on or inside eggs, but further egg studies will be necessary to elucidate the possibility of vertical transmission of the pathogen. Flavobacterium psychrophilum was isolated from water samples, but only from water that had been in close contact with farmed rainbow trout or eggs. Flavobacterium psychrophilum isolates were characterized and compared with well-characterized strains, using degradation of elastin, serotype and ribotype profiles. Different ribotypes of F. psychrophilum were found between hatcheries, but a common ribotype A was found at all four hatcheries. Different ribotypes were found in broodstock without clinical disease, whereas only a few ribotypes (mostly ribotype A) were found in diseased fry. The same ribotype A was found in broodstock, in water samples from hatchery trays and in fry, which suggests the possibility of transmission of F. psychrophilum between broodstock and offspring.