发酵豆粕、肉骨粉及其混合物替代鱼粉对大口黑鲈生长、血清生化指标和肠道组织学的影响

为探究2种蛋白源单一和混合替代鱼粉对大口黑鲈生长性能、血清生化指标和肠道组织学的影响,实验用发酵豆粕(FSM)、肉骨粉(MBM)和发酵豆粕+肉骨粉(FSM+MBM)混合物(1∶1)等蛋白替代基础饲料(FM,含鱼粉350 g/kg)中150 g/kg的鱼粉,使鱼粉含量降为200 g/kg,共配制成4种等氮等脂的实验饲料,记为FM (对照组)、FSM、MBM和FSM+MBM组,投喂初始体质量为(21.2±0.1) g的大口黑鲈8周。结果显示,与对照组相比,各替代组的增重率均无显著差异,但FSM组的增重率显著低于FSM+MBM组。此外,FSM组的饲料系数较对照组和MBM组显著升高。各组在全鱼成分(水分、粗蛋白、粗脂肪、粗灰分)和干物质消化率、蛋白质消化率以及蛋白质沉积率上无显著差异,而FSM组的蛋白质效率和脂肪沉积率显著低于对照组。此外,FSM组的血清谷草转氨酶显著增加,而血清总抗氧化能力以及超氧化物歧化酶、溶菌酶活性显著降低。在前肠形态学方面,各替代组的绒毛宽度均显著低于对照组,FSM组的绒毛高度、肌层厚度以及绒毛周长显著高于对照组。以上结果表明,在鱼粉含量为350 g/kg的饲料中,MB...     

Anti-Allergic Effect of 3,4-Dihydroxybenzaldehyde Isolated from Polysiphonia morrowii in IgE/BSA-Stimulated Mast Cells and a Passive Cutaneous Anaphylaxis Mouse Model

In this study, we investigated the anti-allergic effects of 3,4-dihydroxybenzaldehyde (DHB) isolated from the marine red alga, Polysiphonia morrowii, in mouse bone-marrow-derived cultured mast cells (BMCMCs) and passive cutaneous anaphylaxis (PCA) in anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-sensitized mice. DHB inhibited IgE/bovine serum albumin (BSA)-induced BMCMCs degranulation by reducing the release of β-hexosaminidase without inducing cytotoxicity. Further, DHB dose-dependently decreased the IgE binding and high-affinity IgE receptor (FcεRI) expression and FcεRI-IgE binding on the surface of BMCMCs. Moreover, DHB suppressed the secretion and/or the expression of the allergic cytokines, interleukin (IL)-4, IL-5, IL-6, IL-13, and tumor necrosis factor (TNF)-α, and the chemokine, thymus activation-regulated chemokine (TARC), by regulating the phosphorylation of IκBα and the translocation of cytoplasmic NF-κB into the nucleus. Furthermore, DHB attenuated the passive cutaneous anaphylactic (PCA) reaction reducing the exuded Evans blue amount in the mouse ear stimulated by IgE/BSA. These results suggest that DHB is a potential therapeutic candidate for the prevention and treatment of type I allergic disorders.     

硒对糖尿病大鼠骨密度影响的初步研究

本试验通过给SD大鼠注射链尿佐菌素(streptozotocin,STZ)破坏其胰脏以复制糖尿病模型,通过在饲料中添加不同浓度和形态的硒研究了硒对骨密度(BMD)的影响.结论:糖尿病大鼠较易继发骨质疏松,其骨密度下降以胫骨、肱骨出现最早;低硒组患鼠骨密度下降加快;高硒组能延缓患鼠骨密度下降;硒的形态对糖尿病患鼠骨密度影响差异不显著(P＞0.05).     

Morphological characterization of Arctic char, Salvelinus alpinus, eggs subjected to rapid post-ovulatory aging at 7 °C

Egg quality in Arctic char, Salvelinus alpinus, kept indoors at 7 °C during spawning season was morphologically classified. Four egg categories based on lipid droplet distributions and egg diameter were characterized. Eggs with homogenous lipid vesicle distributions and a uniform size were classified as Good. Eggs with some yolk lipid droplets coalesced toward one pole but were homogeneous in size were classified as Fair. Eggs with lipid vesicles that were usually coalesced at one or two poles with were classified as Poor. The fourth egg category was Heterogeneous, in which lipid vesicle distribution and egg size were inhomogeneous. This distribution pattern of the lipid vesicle had a strong relationship with the percentage of fertilized and eyed eggs. Percentages of fertilized and eyed eggs in Good and Fair eggs were 85.5 and 83.4; 30.2 and 28.2, respectively. With Poor and Heterogeneous eggs, the percentages of fertilized and eyed eggs were zero. Egg diameter and absolute weight was increased in Poor eggs than in other egg categories. Relative weight increase during hardening after 30, 45 and 60 min were higher in Good and Fair eggs than in Poor and Heterogeneous eggs. Ovarian fluid collected from egg batches with low rates of fertilized and eyed eggs (Heterogeneous and Poor) had a lower pH and higher protein and aspartate amino-transferase enzyme than ovarian fluid from eggs batches classified as Good or Fair, while the ovarian fluid osmolality did not significantly differ. ATP contents of Poor and Heterogeneous eggs were lower than for Good and Fair eggs. In Heterogeneous eggs, both ATP and acid phosphatase contents were very variable.     

同位素和微量元素分析在古代人类食物结构研究中的应用

本研究通过对山东沭河上游大汶口文化晚期及春秋战国时期2块人骨的碳、氮同位素分析和无机成分的锶、钡、钙等微量元素分析,探讨了同位素和微量元素分析在古代人类食物结构研究中的应用问题。研究结果表明同位素分析结果能够揭示当时人的食物到底以C3植物、C4植物、食草动物、海产资源的哪个为主这一问题,而微量元素分析结果能够在同位素分析所分类的大类型中,进一步探讨具体的动植物种类。     

Controls for trout and char migratory/resident behaviour mainly in stream systems above and below waterfalls/barriers: a multidecadal and broad geographical review

Northcote TG. Controls for trout and char migratory/resident behaviour mainly in stream systems above and below waterfalls/barriers: a multidecadal and broad geographical review. Ecology of Freshwater Fish 2010: 19: 487–509. © 2010 John Wiley & Sons A/S Abstract – Controls for trout and char migratory and resident behaviour in rivers and streams for above and below natural and man‐made waterfalls/barriers are covered in a multi‐decadal (1950–2000) and wide geographical review (North America, UK, Europe, Japan) that includes reference to over 380 publications. These note 53 for rainbow trout, 61 for cutthroat trout, 104 for brown trout, 47 for bull trout, 41 for brook trout, 35 for Dolly Varden and 42 for white‐spotted char, plus a few general contributions of relevance on some. For each of these species, there has been a major increase in relevant decadal publications since the early 1980s, no doubt in large part because of the upsurge in micro‐genetic methodology for DNA and related technology, coupled with a broadening of interest in stream migratory behaviour of salmonids. Main mechanisms for the control of stream migratory versus resident behaviour appear to cover an interplay among both genetic and environmental factors; in some populations and locations, genetic controls seem to be more important than environmental ones, but in others the reverse. Habitat degradation by various human activities and their introductions of non‐native fish species are becoming causes of reduction in abundance of above and below waterfall stream populations for several of these salmonid species.     

益生乳酸菌对肉鸡骨骼发育的影响及作用机制研究进展

肉鸡产业在我国占有非常重要的地位,益生乳酸菌作为抗生素的有效替代物,正越来越多地应用于肉鸡生产.骨骼质量关乎动物福利、生产效率以及经济效益,其重要性不言而喻.然而,人们往往更多关注肉鸡的肉品质,对骨骼质量的重要性认识不足,且很少重视益生乳酸菌对肉鸡骨骼质量的影响.因此,本文详述了肉鸡骨骼发育的机制及骨骼健康的重要性、益...     

Summer habitat use of Arctic char (Salvelinus alpinus) in a small Arctic lake, monitored by acoustic telemetry

Abstract –  Acoustic technologies were applied to describe how landlocked Arctic char from Iqalugaajuruluit Lake, Baffin Island, interact with its lacustrine habitat. Acoustic data from the lake bottom was collected using sonar equipment and substrate types were verified with benthic grabs and mapped in a geographic information system. Arctic char movements during the open water period were recorded from char fitted with acoustic tags. The distribution of the tagged Arctic char in Iqalugaajuruluit Lake was dependent on fish size and related to abiotic factors such as depth, substrate type and depth/temperature, temporally. The volume of water with temperatures below 6 °C during the open water period may be a limiting factor for large char (>400 mm) in small Arctic lakes. The large piscivorous char are found most often in the deepest water over soft substrates and the smaller char which feed on varying proportions of invertebrates and fish were found most often over the more complex substrates such as boulders, pebbles and gravel.     

骨碎补总黄酮对缺氧环境中犬骨髓间充质干细胞成骨分化潜能的影响

拟研究骨碎补总黄酮对缺氧环境中犬骨髓间充质干细胞(BMSCs)成骨分化潜能的影响.运用骨碎补总黄酮(TFDR)干预低氧浓度(10％)环境中犬BMSCs 4周后诱导成骨分化,倒置显微镜观察茜素红染色BMSCs钙结节形成,比色法检测碱性磷酸酶(ALP)活性水平,流式细胞术检测细胞线粒体膜电位,激光共聚焦显微镜和RT-PCR...     

Unbalanced nutrient ratios in pelleted compound recycling fertilizers

The aim of this study was to contribute to the development of pelleted compound recycling fertilizers with favourable handling and spreading characteristics and balanced nutrient ratios by combining nitrogen (N)‐ and phosphorus (P)‐rich waste resources (meat bone meal, fish sludge or food waste) with potassium (K)‐rich bottom wood ash. Pelleted compound recycling fertilizers with good durability and low dusting tendency were produced by roll‐pelleting preheated waste resources at a suitable moisture content. However, the nutrient ratios in the final products were insufficiently balanced, with too low N concentrations relative to P and K to meet crop demands. In a bioassay using barley (Hordeum vulgare ) and a nutrient‐deficient sand/peat mixture, the relative agronomic effectiveness (RAE ) of pelleted compound recycling fertilizers and reference recycling fertilizers was 22–42% of that of mineral compound fertilizer. Growth limitation was due to reduced N availability (mineral fertilizer equivalent ‐ MFE = 35–57%) or reduced P availability (MFE  = 20–115%), with the greatest P fertilizer value obtained for digestate based on dairy manure and fish sludge. Availability of K in bottom wood ash was masked by the experimental soil.